An ELISA method using serum derived HDAg for the sorological detection of HDV antigens and antibodies
One of the main difficulties related to the detection of the Hepatitis Delta Virus (HDV) antigen and antibody has been the source of the needed HD antigen since HDV containing human and animal livers are very difficult to obtain and since yield is low. This fact prompted us to try to use the serum o...
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Universidade de São Paulo (USP)
1987
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ftdoajarticles:oai:doaj.org/article:64cc442618dc4a2183d00729830bba64 2024-09-09T19:27:30+00:00 An ELISA method using serum derived HDAg for the sorological detection of HDV antigens and antibodies Celso Granato Edna Strauss Ludmila Vitvitsky Luiz Carlos da Costa Gayotto Christian Trepo 1987-12-01T00:00:00Z https://doi.org/10.1590/S0036-46651987000600010 https://doaj.org/article/64cc442618dc4a2183d00729830bba64 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651987000600010&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46651987000600010 https://doaj.org/article/64cc442618dc4a2183d00729830bba64 Revista do Instituto de Medicina Tropical de São Paulo, Vol 29, Iss 6, Pp 388-391 (1987) Hepatitis Hepatitis delta virus Enzyme immuno assay Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1987 ftdoajarticles https://doi.org/10.1590/S0036-46651987000600010 2024-08-05T17:49:30Z One of the main difficulties related to the detection of the Hepatitis Delta Virus (HDV) antigen and antibody has been the source of the needed HD antigen since HDV containing human and animal livers are very difficult to obtain and since yield is low. This fact prompted us to try to use the serum of patients in the acute phase of HDV infection as a source of HDAg and turn to enzyme immunoassays (EIA) instead of RIA for the sake of easiness and economy in the amount of HDAg needed. The antigen for EIA was obtained from patients during the acute phase of HDV infection and the antibody from patients who have been carriers for many years. For the detection of the antigen, a sandwich type method was employed, whereas for the antibody a competition assay was developed. In order to assess the relative specificity and sensibility of the test, the antibody assay was compared to a commercial RIA (C. RIA, Abbott) and to a non-commercial RIA (NC RIA). Forty-two sera were tested by the two methods and only in two cases discrepant results were obtained. Its is concluded that: 1) sera from patients in the acute and chronic phases of HDV infection can be used as source of both antigen and antibody, for immunoassays; 2) EIA and RIA have comparable relative specificity and sensibility and 3) EIA is easier to perform, cheaper, non-hazardous, has a longer shelf-life and saves scarce HDAg. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Abbott ENVELOPE(-62.133,-62.133,-64.100,-64.100) Arctic Eia ENVELOPE(7.755,7.755,63.024,63.024) Revista do Instituto de Medicina Tropical de São Paulo 29 6 388 391 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Hepatitis Hepatitis delta virus Enzyme immuno assay Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Hepatitis Hepatitis delta virus Enzyme immuno assay Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Celso Granato Edna Strauss Ludmila Vitvitsky Luiz Carlos da Costa Gayotto Christian Trepo An ELISA method using serum derived HDAg for the sorological detection of HDV antigens and antibodies |
topic_facet |
Hepatitis Hepatitis delta virus Enzyme immuno assay Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
One of the main difficulties related to the detection of the Hepatitis Delta Virus (HDV) antigen and antibody has been the source of the needed HD antigen since HDV containing human and animal livers are very difficult to obtain and since yield is low. This fact prompted us to try to use the serum of patients in the acute phase of HDV infection as a source of HDAg and turn to enzyme immunoassays (EIA) instead of RIA for the sake of easiness and economy in the amount of HDAg needed. The antigen for EIA was obtained from patients during the acute phase of HDV infection and the antibody from patients who have been carriers for many years. For the detection of the antigen, a sandwich type method was employed, whereas for the antibody a competition assay was developed. In order to assess the relative specificity and sensibility of the test, the antibody assay was compared to a commercial RIA (C. RIA, Abbott) and to a non-commercial RIA (NC RIA). Forty-two sera were tested by the two methods and only in two cases discrepant results were obtained. Its is concluded that: 1) sera from patients in the acute and chronic phases of HDV infection can be used as source of both antigen and antibody, for immunoassays; 2) EIA and RIA have comparable relative specificity and sensibility and 3) EIA is easier to perform, cheaper, non-hazardous, has a longer shelf-life and saves scarce HDAg. |
format |
Article in Journal/Newspaper |
author |
Celso Granato Edna Strauss Ludmila Vitvitsky Luiz Carlos da Costa Gayotto Christian Trepo |
author_facet |
Celso Granato Edna Strauss Ludmila Vitvitsky Luiz Carlos da Costa Gayotto Christian Trepo |
author_sort |
Celso Granato |
title |
An ELISA method using serum derived HDAg for the sorological detection of HDV antigens and antibodies |
title_short |
An ELISA method using serum derived HDAg for the sorological detection of HDV antigens and antibodies |
title_full |
An ELISA method using serum derived HDAg for the sorological detection of HDV antigens and antibodies |
title_fullStr |
An ELISA method using serum derived HDAg for the sorological detection of HDV antigens and antibodies |
title_full_unstemmed |
An ELISA method using serum derived HDAg for the sorological detection of HDV antigens and antibodies |
title_sort |
elisa method using serum derived hdag for the sorological detection of hdv antigens and antibodies |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1987 |
url |
https://doi.org/10.1590/S0036-46651987000600010 https://doaj.org/article/64cc442618dc4a2183d00729830bba64 |
long_lat |
ENVELOPE(-62.133,-62.133,-64.100,-64.100) ENVELOPE(7.755,7.755,63.024,63.024) |
geographic |
Abbott Arctic Eia |
geographic_facet |
Abbott Arctic Eia |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 29, Iss 6, Pp 388-391 (1987) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651987000600010&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46651987000600010 https://doaj.org/article/64cc442618dc4a2183d00729830bba64 |
op_doi |
https://doi.org/10.1590/S0036-46651987000600010 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
29 |
container_issue |
6 |
container_start_page |
388 |
op_container_end_page |
391 |
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1809896925325950976 |