Establishment and long-term culture of the cell lines derived from gonad tissues of Siberian sturgeon (Acipenser baerii)
Abstract To culture germline stem cells in vitro, establishment of the cell lines that can be used as the feeder cells is a prerequisite. In this study, we tried to establish gonad-derived cell lines in Siberian sturgeon (Acipenser baerii). Five 1-year-old A. baerii were used as a donor of gonad tis...
Published in: | Fisheries and Aquatic Sciences |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
The Korean Society of Fisheries and Aquatic Science
2016
|
Subjects: | |
Online Access: | https://doi.org/10.1186/s41240-016-0015-y https://doaj.org/article/6466666ed613491a8f1cdb63e9ebd69d |
id |
ftdoajarticles:oai:doaj.org/article:6466666ed613491a8f1cdb63e9ebd69d |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:6466666ed613491a8f1cdb63e9ebd69d 2023-05-15T13:01:38+02:00 Establishment and long-term culture of the cell lines derived from gonad tissues of Siberian sturgeon (Acipenser baerii) Jun Hyung Ryu Yoon Kwon Nam Seung Pyo Gong 2016-06-01T00:00:00Z https://doi.org/10.1186/s41240-016-0015-y https://doaj.org/article/6466666ed613491a8f1cdb63e9ebd69d EN eng The Korean Society of Fisheries and Aquatic Science http://link.springer.com/article/10.1186/s41240-016-0015-y https://doaj.org/toc/2234-1757 doi:10.1186/s41240-016-0015-y 2234-1757 https://doaj.org/article/6466666ed613491a8f1cdb63e9ebd69d Fisheries and Aquatic Sciences, Vol 19, Iss 1, Pp 1-8 (2016) Sturgeon Acipenser baerii Feeder cells Long-term culture Germline stem cells Aquaculture. Fisheries. Angling SH1-691 article 2016 ftdoajarticles https://doi.org/10.1186/s41240-016-0015-y 2022-12-31T06:56:06Z Abstract To culture germline stem cells in vitro, establishment of the cell lines that can be used as the feeder cells is a prerequisite. In this study, we tried to establish gonad-derived cell lines in Siberian sturgeon (Acipenser baerii). Five 1-year-old A. baerii were used as a donor of gonad tissues, and gonad-dissociated cells were cultured in vitro. Subsequently, determination of growth conditions, long-term culture, characterization, and cryopreservation of the cell lines were also conducted. Five gonad-derived cell lines were stably established and cultured continuously over at least the 73th passage and 402 culture days under the media containing 20 % fetal bovine serum at 28 °C. All cell lines consisted of two main cell types based on morphology even if the ratio of the two cell types was different depending on cell lines. Despite long-term culture, all cell lines maintained diploid DNA contents and expression of several genes that are known to express in the A. baerii gonad. After freezing and thawing of the cell lines, post-thaw cell viabilities between 57.6 and 92.9 % depending on cell lines were indentified, suggesting that stable cryopreservation is possible. The results and the cell lines established in this study will contribute to the development of an in vitro system for A. baerii germline stem cell culture. Article in Journal/Newspaper Acipenser baerii Siberian sturgeon Directory of Open Access Journals: DOAJ Articles Fisheries and Aquatic Sciences 19 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Sturgeon Acipenser baerii Feeder cells Long-term culture Germline stem cells Aquaculture. Fisheries. Angling SH1-691 |
spellingShingle |
Sturgeon Acipenser baerii Feeder cells Long-term culture Germline stem cells Aquaculture. Fisheries. Angling SH1-691 Jun Hyung Ryu Yoon Kwon Nam Seung Pyo Gong Establishment and long-term culture of the cell lines derived from gonad tissues of Siberian sturgeon (Acipenser baerii) |
topic_facet |
Sturgeon Acipenser baerii Feeder cells Long-term culture Germline stem cells Aquaculture. Fisheries. Angling SH1-691 |
description |
Abstract To culture germline stem cells in vitro, establishment of the cell lines that can be used as the feeder cells is a prerequisite. In this study, we tried to establish gonad-derived cell lines in Siberian sturgeon (Acipenser baerii). Five 1-year-old A. baerii were used as a donor of gonad tissues, and gonad-dissociated cells were cultured in vitro. Subsequently, determination of growth conditions, long-term culture, characterization, and cryopreservation of the cell lines were also conducted. Five gonad-derived cell lines were stably established and cultured continuously over at least the 73th passage and 402 culture days under the media containing 20 % fetal bovine serum at 28 °C. All cell lines consisted of two main cell types based on morphology even if the ratio of the two cell types was different depending on cell lines. Despite long-term culture, all cell lines maintained diploid DNA contents and expression of several genes that are known to express in the A. baerii gonad. After freezing and thawing of the cell lines, post-thaw cell viabilities between 57.6 and 92.9 % depending on cell lines were indentified, suggesting that stable cryopreservation is possible. The results and the cell lines established in this study will contribute to the development of an in vitro system for A. baerii germline stem cell culture. |
format |
Article in Journal/Newspaper |
author |
Jun Hyung Ryu Yoon Kwon Nam Seung Pyo Gong |
author_facet |
Jun Hyung Ryu Yoon Kwon Nam Seung Pyo Gong |
author_sort |
Jun Hyung Ryu |
title |
Establishment and long-term culture of the cell lines derived from gonad tissues of Siberian sturgeon (Acipenser baerii) |
title_short |
Establishment and long-term culture of the cell lines derived from gonad tissues of Siberian sturgeon (Acipenser baerii) |
title_full |
Establishment and long-term culture of the cell lines derived from gonad tissues of Siberian sturgeon (Acipenser baerii) |
title_fullStr |
Establishment and long-term culture of the cell lines derived from gonad tissues of Siberian sturgeon (Acipenser baerii) |
title_full_unstemmed |
Establishment and long-term culture of the cell lines derived from gonad tissues of Siberian sturgeon (Acipenser baerii) |
title_sort |
establishment and long-term culture of the cell lines derived from gonad tissues of siberian sturgeon (acipenser baerii) |
publisher |
The Korean Society of Fisheries and Aquatic Science |
publishDate |
2016 |
url |
https://doi.org/10.1186/s41240-016-0015-y https://doaj.org/article/6466666ed613491a8f1cdb63e9ebd69d |
genre |
Acipenser baerii Siberian sturgeon |
genre_facet |
Acipenser baerii Siberian sturgeon |
op_source |
Fisheries and Aquatic Sciences, Vol 19, Iss 1, Pp 1-8 (2016) |
op_relation |
http://link.springer.com/article/10.1186/s41240-016-0015-y https://doaj.org/toc/2234-1757 doi:10.1186/s41240-016-0015-y 2234-1757 https://doaj.org/article/6466666ed613491a8f1cdb63e9ebd69d |
op_doi |
https://doi.org/10.1186/s41240-016-0015-y |
container_title |
Fisheries and Aquatic Sciences |
container_volume |
19 |
container_issue |
1 |
_version_ |
1766282297724633088 |