Effective and cheap removal of leukocytes and platelets from Plasmodium vivax infected blood
Abstract Background Investigations of Plasmodium vivax are restricted to samples collected from infected persons or primates, because this parasite cannot be maintained in in vitro cultures. Contamination of P. vivax isolates with host leukocytes and platelets is detrimental to a range of ex vivo an...
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ftdoajarticles:oai:doaj.org/article:64659d3b730144a6ab069621b55628bb 2023-05-15T15:12:16+02:00 Effective and cheap removal of leukocytes and platelets from Plasmodium vivax infected blood Snounou Georges Phyo Aung Leimanis Mara L Lek-Uthai Usa Suwanarusk Rossarin Kaewpongsri Supaporn Sriprawat Kanlaya Russell Bruce Renia Laurent Nosten François 2009-06-01T00:00:00Z https://doi.org/10.1186/1475-2875-8-115 https://doaj.org/article/64659d3b730144a6ab069621b55628bb EN eng BMC http://www.malariajournal.com/content/8/1/115 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-8-115 1475-2875 https://doaj.org/article/64659d3b730144a6ab069621b55628bb Malaria Journal, Vol 8, Iss 1, p 115 (2009) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2009 ftdoajarticles https://doi.org/10.1186/1475-2875-8-115 2022-12-31T13:11:16Z Abstract Background Investigations of Plasmodium vivax are restricted to samples collected from infected persons or primates, because this parasite cannot be maintained in in vitro cultures. Contamination of P. vivax isolates with host leukocytes and platelets is detrimental to a range of ex vivo and molecular investigations. Easy-to-produce CF11 cellulose filters have recently provided us with an inexpensive method for the removal of leukocytes and platelets. This contrasted with previous reports of unacceptably high levels of infected red blood cell (IRBC) retention by CF11. The aims of this study were to compare the ability of CF11 cellulose filters and the commercial filter Plasmodipur at removing leukocyte and platelet, and to investigate the retention of P. vivax IRBCs by CF11 cellulose filtration. Methods and Results Side-by-side comparison of six leukocyte removal methods using blood samples from five healthy donor showed that CF11 filtration reduced the mean initial leukocyte counts from 9.4 × 10 3 per μl [95%CI 5.2–13.5] to 0.01 × 10 3 [95%CI 0.01–0.03]. The CF11 was particularly effective at removing neutrophils. CF11 treatment also reduced initial platelet counts from 211.6 × 10 3 per μl [95%CI 107.5–315.7] to 0.8 × 10 3 per μl [95%CI -0.7–2.2]. Analysis of 30 P. vivax blood samples before and after CF11 filtration showed only a minor loss in parasitaemia (≤ 7.1% of initial counts). Stage specific retention of P. vivax IRBCs was not observed. Conclusion CF11 filtration is the most cost and time efficient method for the production of leukocyte- and platelet-free P. vivax -infected erythrocytes from field isolates. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 8 1 115 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Snounou Georges Phyo Aung Leimanis Mara L Lek-Uthai Usa Suwanarusk Rossarin Kaewpongsri Supaporn Sriprawat Kanlaya Russell Bruce Renia Laurent Nosten François Effective and cheap removal of leukocytes and platelets from Plasmodium vivax infected blood |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Investigations of Plasmodium vivax are restricted to samples collected from infected persons or primates, because this parasite cannot be maintained in in vitro cultures. Contamination of P. vivax isolates with host leukocytes and platelets is detrimental to a range of ex vivo and molecular investigations. Easy-to-produce CF11 cellulose filters have recently provided us with an inexpensive method for the removal of leukocytes and platelets. This contrasted with previous reports of unacceptably high levels of infected red blood cell (IRBC) retention by CF11. The aims of this study were to compare the ability of CF11 cellulose filters and the commercial filter Plasmodipur at removing leukocyte and platelet, and to investigate the retention of P. vivax IRBCs by CF11 cellulose filtration. Methods and Results Side-by-side comparison of six leukocyte removal methods using blood samples from five healthy donor showed that CF11 filtration reduced the mean initial leukocyte counts from 9.4 × 10 3 per μl [95%CI 5.2–13.5] to 0.01 × 10 3 [95%CI 0.01–0.03]. The CF11 was particularly effective at removing neutrophils. CF11 treatment also reduced initial platelet counts from 211.6 × 10 3 per μl [95%CI 107.5–315.7] to 0.8 × 10 3 per μl [95%CI -0.7–2.2]. Analysis of 30 P. vivax blood samples before and after CF11 filtration showed only a minor loss in parasitaemia (≤ 7.1% of initial counts). Stage specific retention of P. vivax IRBCs was not observed. Conclusion CF11 filtration is the most cost and time efficient method for the production of leukocyte- and platelet-free P. vivax -infected erythrocytes from field isolates. |
format |
Article in Journal/Newspaper |
author |
Snounou Georges Phyo Aung Leimanis Mara L Lek-Uthai Usa Suwanarusk Rossarin Kaewpongsri Supaporn Sriprawat Kanlaya Russell Bruce Renia Laurent Nosten François |
author_facet |
Snounou Georges Phyo Aung Leimanis Mara L Lek-Uthai Usa Suwanarusk Rossarin Kaewpongsri Supaporn Sriprawat Kanlaya Russell Bruce Renia Laurent Nosten François |
author_sort |
Snounou Georges |
title |
Effective and cheap removal of leukocytes and platelets from Plasmodium vivax infected blood |
title_short |
Effective and cheap removal of leukocytes and platelets from Plasmodium vivax infected blood |
title_full |
Effective and cheap removal of leukocytes and platelets from Plasmodium vivax infected blood |
title_fullStr |
Effective and cheap removal of leukocytes and platelets from Plasmodium vivax infected blood |
title_full_unstemmed |
Effective and cheap removal of leukocytes and platelets from Plasmodium vivax infected blood |
title_sort |
effective and cheap removal of leukocytes and platelets from plasmodium vivax infected blood |
publisher |
BMC |
publishDate |
2009 |
url |
https://doi.org/10.1186/1475-2875-8-115 https://doaj.org/article/64659d3b730144a6ab069621b55628bb |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 8, Iss 1, p 115 (2009) |
op_relation |
http://www.malariajournal.com/content/8/1/115 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-8-115 1475-2875 https://doaj.org/article/64659d3b730144a6ab069621b55628bb |
op_doi |
https://doi.org/10.1186/1475-2875-8-115 |
container_title |
Malaria Journal |
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8 |
container_issue |
1 |
container_start_page |
115 |
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1766342980908613632 |