Is qPCR always the most sensitive method for malaria diagnostic quality surveillance?

Abstract In many studies to evaluate the quality of malaria diagnosis, microscopy or rapid diagnostic tests (RDT) are compared to PCR. Depending on the method for sample collection and storage (whole blood or dried blood spot), volume of blood used for extraction, volume of DNA used as PCR template,...

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Bibliographic Details
Published in:Malaria Journal
Main Author: Cristian Koepfli
Format: Article in Journal/Newspaper
Language:English
Published: BMC 2023
Subjects:
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Arctic
Online Access:https://doi.org/10.1186/s12936-023-04822-w
https://doaj.org/article/644b46ffe08445319fc584956d8c3b91
id ftdoajarticles:oai:doaj.org/article:644b46ffe08445319fc584956d8c3b91
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spelling ftdoajarticles:oai:doaj.org/article:644b46ffe08445319fc584956d8c3b91 2024-01-21T10:03:39+01:00 Is qPCR always the most sensitive method for malaria diagnostic quality surveillance? Cristian Koepfli 2023-12-01T00:00:00Z https://doi.org/10.1186/s12936-023-04822-w https://doaj.org/article/644b46ffe08445319fc584956d8c3b91 EN eng BMC https://doi.org/10.1186/s12936-023-04822-w https://doaj.org/toc/1475-2875 doi:10.1186/s12936-023-04822-w 1475-2875 https://doaj.org/article/644b46ffe08445319fc584956d8c3b91 Malaria Journal, Vol 22, Iss 1, Pp 1-3 (2023) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2023 ftdoajarticles https://doi.org/10.1186/s12936-023-04822-w 2023-12-24T01:47:20Z Abstract In many studies to evaluate the quality of malaria diagnosis, microscopy or rapid diagnostic tests (RDT) are compared to PCR. Depending on the method for sample collection and storage (whole blood or dried blood spot), volume of blood used for extraction, volume of DNA used as PCR template, and choice of PCR target (single vs. multi-copy gene), the limit of detection (LOD) of PCR might not exceed the LOD of expert microscopy or RDT. One should not assume that PCR always detects the highest number of infections. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 22 1
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
spellingShingle Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Cristian Koepfli
Is qPCR always the most sensitive method for malaria diagnostic quality surveillance?
topic_facet Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
description Abstract In many studies to evaluate the quality of malaria diagnosis, microscopy or rapid diagnostic tests (RDT) are compared to PCR. Depending on the method for sample collection and storage (whole blood or dried blood spot), volume of blood used for extraction, volume of DNA used as PCR template, and choice of PCR target (single vs. multi-copy gene), the limit of detection (LOD) of PCR might not exceed the LOD of expert microscopy or RDT. One should not assume that PCR always detects the highest number of infections.
format Article in Journal/Newspaper
author Cristian Koepfli
author_facet Cristian Koepfli
author_sort Cristian Koepfli
title Is qPCR always the most sensitive method for malaria diagnostic quality surveillance?
title_short Is qPCR always the most sensitive method for malaria diagnostic quality surveillance?
title_full Is qPCR always the most sensitive method for malaria diagnostic quality surveillance?
title_fullStr Is qPCR always the most sensitive method for malaria diagnostic quality surveillance?
title_full_unstemmed Is qPCR always the most sensitive method for malaria diagnostic quality surveillance?
title_sort is qpcr always the most sensitive method for malaria diagnostic quality surveillance?
publisher BMC
publishDate 2023
url https://doi.org/10.1186/s12936-023-04822-w
https://doaj.org/article/644b46ffe08445319fc584956d8c3b91
geographic Arctic
geographic_facet Arctic
genre Arctic
genre_facet Arctic
op_source Malaria Journal, Vol 22, Iss 1, Pp 1-3 (2023)
op_relation https://doi.org/10.1186/s12936-023-04822-w
https://doaj.org/toc/1475-2875
doi:10.1186/s12936-023-04822-w
1475-2875
https://doaj.org/article/644b46ffe08445319fc584956d8c3b91
op_doi https://doi.org/10.1186/s12936-023-04822-w
container_title Malaria Journal
container_volume 22
container_issue 1
_version_ 1788693953208385536

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