Histopathological effects of water soluble–fraction of crude oil on liver tissue of fingerling beluga , Huso huso Linnaeus, 1754
In this study the LC50 96h values of water-soluble fractions (WSF) of the Caspian Sea – exploited crude oil were determined in beluga, Huso huso fingerlings. The fingerlings averaged 2.42 ± 0.11 g in weight were exposed to WSF crude oil at concentrations 24.75, 27, 29.25, 31.5, 33.75, 36, 38.25 ppm....
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ftdoajarticles:oai:doaj.org/article:64301d463924451e9739a8c38da5f567 2023-05-15T15:41:41+02:00 Histopathological effects of water soluble–fraction of crude oil on liver tissue of fingerling beluga , Huso huso Linnaeus, 1754 Khabakhsh, E. Jamili, Sh. Motalebi, A. Mashinchian moradi, A. Nasrolahzade Saravi, A. 2014-10-01T00:00:00Z https://doaj.org/article/64301d463924451e9739a8c38da5f567 EN eng University of Guilan https://cjes.guilan.ac.ir/article_1134.html https://doaj.org/toc/1735-3033 https://doaj.org/toc/1735-3866 1735-3033 1735-3866 https://doaj.org/article/64301d463924451e9739a8c38da5f567 Caspian Journal of Environmental Sciences, Vol 12, Iss 1, Pp 63-72 (2014) Histology Caspian Sea WSF of crude oil Huso huso Beluga Environmental sciences GE1-350 Science Q article 2014 ftdoajarticles 2022-12-31T01:21:41Z In this study the LC50 96h values of water-soluble fractions (WSF) of the Caspian Sea – exploited crude oil were determined in beluga, Huso huso fingerlings. The fingerlings averaged 2.42 ± 0.11 g in weight were exposed to WSF crude oil at concentrations 24.75, 27, 29.25, 31.5, 33.75, 36, 38.25 ppm. The treatments were performed in three replicates and all changes in the specimens were determined for each concentration. Water quality parameters of the seawater were tested and recorded. The LC50 96h value was found to be 34.87 ppm. In the other step, the fingerling beluga was exposed to three sub-lethal concentrations (13.93, 20.92, 27.90 ppm) of WSF of the Caspian crude oil representing 40, 60 and 80% of LC50 96h respectively, and control without crude oil. All treatments and control were conducted in triplicate. Three specimens of each treatment were sampled for histological studies every day. Results revealed that the fish liver tissue of all the treatments showed histological changes, in comparison to the control after 2 days. Even if the changes in the treatment exposed to 27.90 ppm (80% LC50 96h) WSF occurred in 24h after exposure. Histopathological findings in liver included cloudy swelling, pyknotic nuclei, Karyorrhectic nuclei, fatty vacuolation, fatty degeneration, hypertrophy of hepatocyte, necrosis, hypertrophy of bile ducts, cholestasis and bile stagnation, inflammation, fibrosis and cirrosis. These alterations were more conspicuous with increasing the soluble fraction of crude oil concentrations and exposing time. The results are of importance since much attention has been paid to the oil slicks, particulate or sedimentary pollutions, instead of potential toxic effects of water-soluble fractions of oil, which are more available to marine biota. Article in Journal/Newspaper Beluga Beluga* Directory of Open Access Journals: DOAJ Articles |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Histology Caspian Sea WSF of crude oil Huso huso Beluga Environmental sciences GE1-350 Science Q |
spellingShingle |
Histology Caspian Sea WSF of crude oil Huso huso Beluga Environmental sciences GE1-350 Science Q Khabakhsh, E. Jamili, Sh. Motalebi, A. Mashinchian moradi, A. Nasrolahzade Saravi, A. Histopathological effects of water soluble–fraction of crude oil on liver tissue of fingerling beluga , Huso huso Linnaeus, 1754 |
topic_facet |
Histology Caspian Sea WSF of crude oil Huso huso Beluga Environmental sciences GE1-350 Science Q |
description |
In this study the LC50 96h values of water-soluble fractions (WSF) of the Caspian Sea – exploited crude oil were determined in beluga, Huso huso fingerlings. The fingerlings averaged 2.42 ± 0.11 g in weight were exposed to WSF crude oil at concentrations 24.75, 27, 29.25, 31.5, 33.75, 36, 38.25 ppm. The treatments were performed in three replicates and all changes in the specimens were determined for each concentration. Water quality parameters of the seawater were tested and recorded. The LC50 96h value was found to be 34.87 ppm. In the other step, the fingerling beluga was exposed to three sub-lethal concentrations (13.93, 20.92, 27.90 ppm) of WSF of the Caspian crude oil representing 40, 60 and 80% of LC50 96h respectively, and control without crude oil. All treatments and control were conducted in triplicate. Three specimens of each treatment were sampled for histological studies every day. Results revealed that the fish liver tissue of all the treatments showed histological changes, in comparison to the control after 2 days. Even if the changes in the treatment exposed to 27.90 ppm (80% LC50 96h) WSF occurred in 24h after exposure. Histopathological findings in liver included cloudy swelling, pyknotic nuclei, Karyorrhectic nuclei, fatty vacuolation, fatty degeneration, hypertrophy of hepatocyte, necrosis, hypertrophy of bile ducts, cholestasis and bile stagnation, inflammation, fibrosis and cirrosis. These alterations were more conspicuous with increasing the soluble fraction of crude oil concentrations and exposing time. The results are of importance since much attention has been paid to the oil slicks, particulate or sedimentary pollutions, instead of potential toxic effects of water-soluble fractions of oil, which are more available to marine biota. |
format |
Article in Journal/Newspaper |
author |
Khabakhsh, E. Jamili, Sh. Motalebi, A. Mashinchian moradi, A. Nasrolahzade Saravi, A. |
author_facet |
Khabakhsh, E. Jamili, Sh. Motalebi, A. Mashinchian moradi, A. Nasrolahzade Saravi, A. |
author_sort |
Khabakhsh, E. |
title |
Histopathological effects of water soluble–fraction of crude oil on liver tissue of fingerling beluga , Huso huso Linnaeus, 1754 |
title_short |
Histopathological effects of water soluble–fraction of crude oil on liver tissue of fingerling beluga , Huso huso Linnaeus, 1754 |
title_full |
Histopathological effects of water soluble–fraction of crude oil on liver tissue of fingerling beluga , Huso huso Linnaeus, 1754 |
title_fullStr |
Histopathological effects of water soluble–fraction of crude oil on liver tissue of fingerling beluga , Huso huso Linnaeus, 1754 |
title_full_unstemmed |
Histopathological effects of water soluble–fraction of crude oil on liver tissue of fingerling beluga , Huso huso Linnaeus, 1754 |
title_sort |
histopathological effects of water soluble–fraction of crude oil on liver tissue of fingerling beluga , huso huso linnaeus, 1754 |
publisher |
University of Guilan |
publishDate |
2014 |
url |
https://doaj.org/article/64301d463924451e9739a8c38da5f567 |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_source |
Caspian Journal of Environmental Sciences, Vol 12, Iss 1, Pp 63-72 (2014) |
op_relation |
https://cjes.guilan.ac.ir/article_1134.html https://doaj.org/toc/1735-3033 https://doaj.org/toc/1735-3866 1735-3033 1735-3866 https://doaj.org/article/64301d463924451e9739a8c38da5f567 |
_version_ |
1766374577858936832 |