Antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L.
Objective: To investigate potential antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L. in different in vivo and in vitro experimental models. Methods: In vitro DPPH radical scavenging assay was used to evaluate the antioxidant activity of the pl...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:63cd19082e77492d87927413caebbd5d 2023-05-15T15:11:54+02:00 Antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L. Nripendra Nath Biswas Subarna Saha Mohammed Khadem Ali 2014-10-01T00:00:00Z https://doi.org/10.12980/APJTB.4.2014C1298 https://doaj.org/article/63cd19082e77492d87927413caebbd5d EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115300198 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C1298 https://doaj.org/article/63cd19082e77492d87927413caebbd5d Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 10, Pp 792-797 (2014) Antioxidant Antibacterial Cytotoxic Analgesic Mentha arvensis L Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2014 ftdoajarticles https://doi.org/10.12980/APJTB.4.2014C1298 2022-12-31T02:14:27Z Objective: To investigate potential antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L. in different in vivo and in vitro experimental models. Methods: In vitro DPPH radical scavenging assay was used to evaluate the antioxidant activity of the plant extract. In vivo analgesic activity was carried out by acetic acid-induced writhing test in Swiss albino mice. All studies in mice were undertaken at the doses of 250 and 500 mg/kg body weight. Antibacterial activity was studied by disk diffusion assay against some Gram-positive and Gram-negative bacterial strains. Brine shrimp lethality assay was used to investigate cytotoxicity effects of the plant extract. Results: The extract showed free radical scavenging activity in the DPPH assay (IC50∼41 μg/mL) compared to the standard antioxidant ascorbic acid (IC50∼19 μg/mL). The extract also produced prominent antimicrobial activity against Salmonella typhi, Salmonella paratyphi, Shigella boydii, Streptococcus pyogenes and Streptococcus aureus compared to standard drug kanamycin at the dose of 30 μg/disc. The extract exhibited lethality against the brine shrimp nauplii with the LC50 values of 40 μg/mL, and also 90% mortality (LC90) value was found to be 160 μg/mL. In analgesic test, the extract demonstrated statistically significant (P<0.01) analgesic effect in acetic acid induced writhing in white albino mice at both dose levels. Conclusions: These results suggest that the ethanolic extract of Mentha arvensis L. has potential antioxidant, antibacterial, cytotoxic and analgesic activities that support the ethnopharmacological uses of this plant. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 4 10 792 797 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antioxidant Antibacterial Cytotoxic Analgesic Mentha arvensis L Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Antioxidant Antibacterial Cytotoxic Analgesic Mentha arvensis L Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Nripendra Nath Biswas Subarna Saha Mohammed Khadem Ali Antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L. |
topic_facet |
Antioxidant Antibacterial Cytotoxic Analgesic Mentha arvensis L Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate potential antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L. in different in vivo and in vitro experimental models. Methods: In vitro DPPH radical scavenging assay was used to evaluate the antioxidant activity of the plant extract. In vivo analgesic activity was carried out by acetic acid-induced writhing test in Swiss albino mice. All studies in mice were undertaken at the doses of 250 and 500 mg/kg body weight. Antibacterial activity was studied by disk diffusion assay against some Gram-positive and Gram-negative bacterial strains. Brine shrimp lethality assay was used to investigate cytotoxicity effects of the plant extract. Results: The extract showed free radical scavenging activity in the DPPH assay (IC50∼41 μg/mL) compared to the standard antioxidant ascorbic acid (IC50∼19 μg/mL). The extract also produced prominent antimicrobial activity against Salmonella typhi, Salmonella paratyphi, Shigella boydii, Streptococcus pyogenes and Streptococcus aureus compared to standard drug kanamycin at the dose of 30 μg/disc. The extract exhibited lethality against the brine shrimp nauplii with the LC50 values of 40 μg/mL, and also 90% mortality (LC90) value was found to be 160 μg/mL. In analgesic test, the extract demonstrated statistically significant (P<0.01) analgesic effect in acetic acid induced writhing in white albino mice at both dose levels. Conclusions: These results suggest that the ethanolic extract of Mentha arvensis L. has potential antioxidant, antibacterial, cytotoxic and analgesic activities that support the ethnopharmacological uses of this plant. |
format |
Article in Journal/Newspaper |
author |
Nripendra Nath Biswas Subarna Saha Mohammed Khadem Ali |
author_facet |
Nripendra Nath Biswas Subarna Saha Mohammed Khadem Ali |
author_sort |
Nripendra Nath Biswas |
title |
Antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L. |
title_short |
Antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L. |
title_full |
Antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L. |
title_fullStr |
Antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L. |
title_full_unstemmed |
Antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L. |
title_sort |
antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of mentha arvensis l. |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2014 |
url |
https://doi.org/10.12980/APJTB.4.2014C1298 https://doaj.org/article/63cd19082e77492d87927413caebbd5d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 10, Pp 792-797 (2014) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115300198 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C1298 https://doaj.org/article/63cd19082e77492d87927413caebbd5d |
op_doi |
https://doi.org/10.12980/APJTB.4.2014C1298 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
4 |
container_issue |
10 |
container_start_page |
792 |
op_container_end_page |
797 |
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1766342677465399296 |