Vector competence and immune response of Aedes aegypti for Ebinur Lake virus, a newly classified mosquito-borne orthobunyavirus.
The global impact of mosquito-borne diseases has increased significantly over recent decades. Ebinur Lake virus (EBIV), a newly classified orthobunyavirus, is reported to be highly pathogenic in adult mice. The evaluation of vector competence is essential for predicting the arbovirus transmission ri...
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ftdoajarticles:oai:doaj.org/article:63bd7f74da2e4059913c88a0bf4698b2 2023-05-15T15:09:34+02:00 Vector competence and immune response of Aedes aegypti for Ebinur Lake virus, a newly classified mosquito-borne orthobunyavirus. Cihan Yang Fei Wang Doudou Huang Haixia Ma Lu Zhao Guilin Zhang Hailong Li Qian Han Dennis Bente Ferdinand Villanueva Salazar Zhiming Yuan Han Xia 2022-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010642 https://doaj.org/article/63bd7f74da2e4059913c88a0bf4698b2 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010642 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010642 https://doaj.org/article/63bd7f74da2e4059913c88a0bf4698b2 PLoS Neglected Tropical Diseases, Vol 16, Iss 7, p e0010642 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010642 2022-12-31T00:41:32Z The global impact of mosquito-borne diseases has increased significantly over recent decades. Ebinur Lake virus (EBIV), a newly classified orthobunyavirus, is reported to be highly pathogenic in adult mice. The evaluation of vector competence is essential for predicting the arbovirus transmission risk. Here, Aedes aegypti was applied to evaluate EBIV infection and dissemination in mosquitos. Our experiments indicated that Ae. aegypti had the possibility to spread EBIV (with a transmission rate of up to 11.8% at 14 days post-infection) through biting, with the highest viral dose in a single mosquito's saliva reaching 6.3 plaque-forming units. The highest infection, dissemination and ovary infection rates were 70%, 42.9%, and 29.4%, respectively. The high viral infection rates in Ae. aegypti ovaries imply the possibility of EBIV vertical transmission. Ae. aegypti was highly susceptible to intrathoracic infection and the saliva-positive rate reached 90% at 10 days post-infection. Transcriptomic analysis revealed Toll and Imd signaling pathways were implicated in the mosquito's defensive response to EBIV infection. Defensin C and chitinase 10 were continuously downregulated in mosquitoes infected via intrathoracic inoculation of EBIV. Comprehensive analysis of the vector competence of Ae. aegypti for EBIV in laboratory has indicated the potential risk of EBIV transmission through mosquitoes. Moreover, our findings support a complex interplay between EBIV and the immune system of mosquito, which could affect its vector competence. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 7 e0010642 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Cihan Yang Fei Wang Doudou Huang Haixia Ma Lu Zhao Guilin Zhang Hailong Li Qian Han Dennis Bente Ferdinand Villanueva Salazar Zhiming Yuan Han Xia Vector competence and immune response of Aedes aegypti for Ebinur Lake virus, a newly classified mosquito-borne orthobunyavirus. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The global impact of mosquito-borne diseases has increased significantly over recent decades. Ebinur Lake virus (EBIV), a newly classified orthobunyavirus, is reported to be highly pathogenic in adult mice. The evaluation of vector competence is essential for predicting the arbovirus transmission risk. Here, Aedes aegypti was applied to evaluate EBIV infection and dissemination in mosquitos. Our experiments indicated that Ae. aegypti had the possibility to spread EBIV (with a transmission rate of up to 11.8% at 14 days post-infection) through biting, with the highest viral dose in a single mosquito's saliva reaching 6.3 plaque-forming units. The highest infection, dissemination and ovary infection rates were 70%, 42.9%, and 29.4%, respectively. The high viral infection rates in Ae. aegypti ovaries imply the possibility of EBIV vertical transmission. Ae. aegypti was highly susceptible to intrathoracic infection and the saliva-positive rate reached 90% at 10 days post-infection. Transcriptomic analysis revealed Toll and Imd signaling pathways were implicated in the mosquito's defensive response to EBIV infection. Defensin C and chitinase 10 were continuously downregulated in mosquitoes infected via intrathoracic inoculation of EBIV. Comprehensive analysis of the vector competence of Ae. aegypti for EBIV in laboratory has indicated the potential risk of EBIV transmission through mosquitoes. Moreover, our findings support a complex interplay between EBIV and the immune system of mosquito, which could affect its vector competence. |
format |
Article in Journal/Newspaper |
author |
Cihan Yang Fei Wang Doudou Huang Haixia Ma Lu Zhao Guilin Zhang Hailong Li Qian Han Dennis Bente Ferdinand Villanueva Salazar Zhiming Yuan Han Xia |
author_facet |
Cihan Yang Fei Wang Doudou Huang Haixia Ma Lu Zhao Guilin Zhang Hailong Li Qian Han Dennis Bente Ferdinand Villanueva Salazar Zhiming Yuan Han Xia |
author_sort |
Cihan Yang |
title |
Vector competence and immune response of Aedes aegypti for Ebinur Lake virus, a newly classified mosquito-borne orthobunyavirus. |
title_short |
Vector competence and immune response of Aedes aegypti for Ebinur Lake virus, a newly classified mosquito-borne orthobunyavirus. |
title_full |
Vector competence and immune response of Aedes aegypti for Ebinur Lake virus, a newly classified mosquito-borne orthobunyavirus. |
title_fullStr |
Vector competence and immune response of Aedes aegypti for Ebinur Lake virus, a newly classified mosquito-borne orthobunyavirus. |
title_full_unstemmed |
Vector competence and immune response of Aedes aegypti for Ebinur Lake virus, a newly classified mosquito-borne orthobunyavirus. |
title_sort |
vector competence and immune response of aedes aegypti for ebinur lake virus, a newly classified mosquito-borne orthobunyavirus. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010642 https://doaj.org/article/63bd7f74da2e4059913c88a0bf4698b2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 7, p e0010642 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010642 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010642 https://doaj.org/article/63bd7f74da2e4059913c88a0bf4698b2 |
op_doi |
https://doi.org/10.1371/journal.pntd.0010642 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
16 |
container_issue |
7 |
container_start_page |
e0010642 |
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1766340740055564288 |