Ocean acidification modulates expression of genes and physiological performance of a marine diatom.
Ocean Acidification (OA) is known to affect various aspects of physiological performances of diatoms, but little is known about the underlining molecular mechanisms involved. Here, we show that in the model diatom Phaeodactylum tricornutum, the expression of key genes associated with photosynthetic...
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ftdoajarticles:oai:doaj.org/article:6381eeab7729450b9e818b11ca464a68 2023-05-15T17:50:45+02:00 Ocean acidification modulates expression of genes and physiological performance of a marine diatom. Yahe Li Shufang Zhuang Yaping Wu Honglin Ren Fangyi Chen Xin Lin Kejian Wang John Beardall Kunshan Gao 2017-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0170970 https://doaj.org/article/6381eeab7729450b9e818b11ca464a68 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5305191?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0170970 https://doaj.org/article/6381eeab7729450b9e818b11ca464a68 PLoS ONE, Vol 12, Iss 2, p e0170970 (2017) Medicine R Science Q article 2017 ftdoajarticles https://doi.org/10.1371/journal.pone.0170970 2022-12-31T16:00:59Z Ocean Acidification (OA) is known to affect various aspects of physiological performances of diatoms, but little is known about the underlining molecular mechanisms involved. Here, we show that in the model diatom Phaeodactylum tricornutum, the expression of key genes associated with photosynthetic light harvesting as well as those encoding Rubisco, carbonic anhydrase, NADH dehydrogenase and nitrite reductase, are modulated by OA (1000 μatm, pHnbs 7.83). Growth and photosynthetic carbon fixation were enhanced by elevated CO2. OA treatment decreased the expression of β-carbonic anhydrase (β-ca), which functions in balancing intracellular carbonate chemistry and the CO2 concentrating mechanism (CCM). The expression of the genes encoding fucoxanthin chlorophyll a/c protein (lhcf type (fcp)), mitochondrial ATP synthase (mtATP), ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit gene (rbcl) and NADH dehydrogenase subunit 2 (ndh2), were down-regulated during the first four days (< 8 generations) after the cells were transferred from LC (cells grown under ambient air condition; 390 μatm; pHnbs 8.19) to OA conditions, with no significant difference between LC and HC treatments with the time elapsed. The expression of nitrite reductase (nir) was up-regulated by the OA treatment. Additionally, the genes for these proteins (NiR, FCP, mtATP synthase, β-CA) showed diel expression patterns. It appeared that the enhanced photosynthetic and growth rates under OA could be attributed to stimulated nitrogen assimilation, increased CO2 availability or saved energy from down-regulation of the CCM and consequently lowered cost of protein synthesis versus that of non-nitrogenous cell components. Article in Journal/Newspaper Ocean acidification Directory of Open Access Journals: DOAJ Articles PLOS ONE 12 2 e0170970 |
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Medicine R Science Q Yahe Li Shufang Zhuang Yaping Wu Honglin Ren Fangyi Chen Xin Lin Kejian Wang John Beardall Kunshan Gao Ocean acidification modulates expression of genes and physiological performance of a marine diatom. |
topic_facet |
Medicine R Science Q |
description |
Ocean Acidification (OA) is known to affect various aspects of physiological performances of diatoms, but little is known about the underlining molecular mechanisms involved. Here, we show that in the model diatom Phaeodactylum tricornutum, the expression of key genes associated with photosynthetic light harvesting as well as those encoding Rubisco, carbonic anhydrase, NADH dehydrogenase and nitrite reductase, are modulated by OA (1000 μatm, pHnbs 7.83). Growth and photosynthetic carbon fixation were enhanced by elevated CO2. OA treatment decreased the expression of β-carbonic anhydrase (β-ca), which functions in balancing intracellular carbonate chemistry and the CO2 concentrating mechanism (CCM). The expression of the genes encoding fucoxanthin chlorophyll a/c protein (lhcf type (fcp)), mitochondrial ATP synthase (mtATP), ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit gene (rbcl) and NADH dehydrogenase subunit 2 (ndh2), were down-regulated during the first four days (< 8 generations) after the cells were transferred from LC (cells grown under ambient air condition; 390 μatm; pHnbs 8.19) to OA conditions, with no significant difference between LC and HC treatments with the time elapsed. The expression of nitrite reductase (nir) was up-regulated by the OA treatment. Additionally, the genes for these proteins (NiR, FCP, mtATP synthase, β-CA) showed diel expression patterns. It appeared that the enhanced photosynthetic and growth rates under OA could be attributed to stimulated nitrogen assimilation, increased CO2 availability or saved energy from down-regulation of the CCM and consequently lowered cost of protein synthesis versus that of non-nitrogenous cell components. |
format |
Article in Journal/Newspaper |
author |
Yahe Li Shufang Zhuang Yaping Wu Honglin Ren Fangyi Chen Xin Lin Kejian Wang John Beardall Kunshan Gao |
author_facet |
Yahe Li Shufang Zhuang Yaping Wu Honglin Ren Fangyi Chen Xin Lin Kejian Wang John Beardall Kunshan Gao |
author_sort |
Yahe Li |
title |
Ocean acidification modulates expression of genes and physiological performance of a marine diatom. |
title_short |
Ocean acidification modulates expression of genes and physiological performance of a marine diatom. |
title_full |
Ocean acidification modulates expression of genes and physiological performance of a marine diatom. |
title_fullStr |
Ocean acidification modulates expression of genes and physiological performance of a marine diatom. |
title_full_unstemmed |
Ocean acidification modulates expression of genes and physiological performance of a marine diatom. |
title_sort |
ocean acidification modulates expression of genes and physiological performance of a marine diatom. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pone.0170970 https://doaj.org/article/6381eeab7729450b9e818b11ca464a68 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
PLoS ONE, Vol 12, Iss 2, p e0170970 (2017) |
op_relation |
http://europepmc.org/articles/PMC5305191?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0170970 https://doaj.org/article/6381eeab7729450b9e818b11ca464a68 |
op_doi |
https://doi.org/10.1371/journal.pone.0170970 |
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PLOS ONE |
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12 |
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2 |
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e0170970 |
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1766157633383825408 |