Leishmania mexicana induces limited recruitment and activation of monocytes and monocyte-derived dendritic cells early during infection.
While C57BL/6 mice infected in the ear with L. major mount a vigorous Th1 response and resolve their lesions, the Th1 response in C57BL/6 mice infected with L. mexicana is more limited, resulting in chronic, non-healing lesions. The aim of this study was to determine if the limited immune response f...
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ftdoajarticles:oai:doaj.org/article:62e241a61cfa4ac9bb3e8f997d0a0e22 2023-05-15T15:10:27+02:00 Leishmania mexicana induces limited recruitment and activation of monocytes and monocyte-derived dendritic cells early during infection. Patricia M Petritus Daniel Manzoni-de-Almeida Ciara Gimblet Claudia Gonzalez Lombana Phillip Scott 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001858 https://doaj.org/article/62e241a61cfa4ac9bb3e8f997d0a0e22 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3475671?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001858 https://doaj.org/article/62e241a61cfa4ac9bb3e8f997d0a0e22 PLoS Neglected Tropical Diseases, Vol 6, Iss 10, p e1858 (2012) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001858 2022-12-31T00:58:55Z While C57BL/6 mice infected in the ear with L. major mount a vigorous Th1 response and resolve their lesions, the Th1 response in C57BL/6 mice infected with L. mexicana is more limited, resulting in chronic, non-healing lesions. The aim of this study was to determine if the limited immune response following infection with L. mexicana is related to a deficiency in the ability of monocyte-derived dendritic cells (mo-DCs) to prime a sufficient Th1 response. To address this issue we compared the early immune response following L. mexicana infection with that seen in L. major infected mice. Our data show that fewer monocytes are recruited to the lesions of L. mexicana infected mice as compared to mice infected with L. major. Moreover, monocytes that differentiate into mo-DCs in L. mexicana lesions produced less iNOS and migrated less efficiently to the draining lymph node as compared to those from L. major infected mice. Treatment of L. mexicana infected mice with α-IL-10R antibody resulted in increased recruitment of monocytes to the lesion along with greater production of IFN-γ and iNOS. Additionally, injection of DCs into the ear at the time of infection with L. mexicana also led to a more robust Th1 response. Taken together, these data suggest that during L. mexicana infection reduced recruitment, activation and subsequent migration of monocytes and mo-DCs to the draining lymph nodes may result in the insufficient priming of a Th1 response. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 6 10 e1858 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Patricia M Petritus Daniel Manzoni-de-Almeida Ciara Gimblet Claudia Gonzalez Lombana Phillip Scott Leishmania mexicana induces limited recruitment and activation of monocytes and monocyte-derived dendritic cells early during infection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
While C57BL/6 mice infected in the ear with L. major mount a vigorous Th1 response and resolve their lesions, the Th1 response in C57BL/6 mice infected with L. mexicana is more limited, resulting in chronic, non-healing lesions. The aim of this study was to determine if the limited immune response following infection with L. mexicana is related to a deficiency in the ability of monocyte-derived dendritic cells (mo-DCs) to prime a sufficient Th1 response. To address this issue we compared the early immune response following L. mexicana infection with that seen in L. major infected mice. Our data show that fewer monocytes are recruited to the lesions of L. mexicana infected mice as compared to mice infected with L. major. Moreover, monocytes that differentiate into mo-DCs in L. mexicana lesions produced less iNOS and migrated less efficiently to the draining lymph node as compared to those from L. major infected mice. Treatment of L. mexicana infected mice with α-IL-10R antibody resulted in increased recruitment of monocytes to the lesion along with greater production of IFN-γ and iNOS. Additionally, injection of DCs into the ear at the time of infection with L. mexicana also led to a more robust Th1 response. Taken together, these data suggest that during L. mexicana infection reduced recruitment, activation and subsequent migration of monocytes and mo-DCs to the draining lymph nodes may result in the insufficient priming of a Th1 response. |
format |
Article in Journal/Newspaper |
author |
Patricia M Petritus Daniel Manzoni-de-Almeida Ciara Gimblet Claudia Gonzalez Lombana Phillip Scott |
author_facet |
Patricia M Petritus Daniel Manzoni-de-Almeida Ciara Gimblet Claudia Gonzalez Lombana Phillip Scott |
author_sort |
Patricia M Petritus |
title |
Leishmania mexicana induces limited recruitment and activation of monocytes and monocyte-derived dendritic cells early during infection. |
title_short |
Leishmania mexicana induces limited recruitment and activation of monocytes and monocyte-derived dendritic cells early during infection. |
title_full |
Leishmania mexicana induces limited recruitment and activation of monocytes and monocyte-derived dendritic cells early during infection. |
title_fullStr |
Leishmania mexicana induces limited recruitment and activation of monocytes and monocyte-derived dendritic cells early during infection. |
title_full_unstemmed |
Leishmania mexicana induces limited recruitment and activation of monocytes and monocyte-derived dendritic cells early during infection. |
title_sort |
leishmania mexicana induces limited recruitment and activation of monocytes and monocyte-derived dendritic cells early during infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pntd.0001858 https://doaj.org/article/62e241a61cfa4ac9bb3e8f997d0a0e22 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 6, Iss 10, p e1858 (2012) |
op_relation |
http://europepmc.org/articles/PMC3475671?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001858 https://doaj.org/article/62e241a61cfa4ac9bb3e8f997d0a0e22 |
op_doi |
https://doi.org/10.1371/journal.pntd.0001858 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
6 |
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10 |
container_start_page |
e1858 |
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