The effect of morphine on development of ulcer lesions of the rats exposed to indomethacin induced stress
Oxidative stress plays an important role in development of ulcer lessions of the rats exposed to indomethacin induced stress. It has been suggested that endogenous opioids releassed during the stress may attenuate gastric ulcer lesions. The aim of this study was to investigate effects of morpine on...
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Medicinski fakultet Priština, Društvo lekara Kosova i Metohije Srpskog lekarskog društva
2018
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ftdoajarticles:oai:doaj.org/article:62c06e6a648448b3b36410926064fdc7 2023-05-15T16:02:08+02:00 The effect of morphine on development of ulcer lesions of the rats exposed to indomethacin induced stress Rašić Julijana Hudomal-Janićijević Snežana Stanojević-Ristić Zorica Kisić Bojana Stević Snežana Vitković Leonida Mijović Milica 2018-01-01T00:00:00Z https://doaj.org/article/62c06e6a648448b3b36410926064fdc7 EN SR eng srp Medicinski fakultet Priština, Društvo lekara Kosova i Metohije Srpskog lekarskog društva https://scindeks-clanci.ceon.rs/data/pdf/0350-8773/2018/0350-87731802001R.pdf https://doaj.org/toc/0350-8773 https://doaj.org/toc/2560-3310 0350-8773 2560-3310 https://doaj.org/article/62c06e6a648448b3b36410926064fdc7 Praxis Medica, Vol 47, Iss 1-2, Pp 1-8 (2018) morphine indomethacin stress ulcer oxidative stress antioxidative status Medicine R article 2018 ftdoajarticles 2022-12-31T01:57:29Z Oxidative stress plays an important role in development of ulcer lessions of the rats exposed to indomethacin induced stress. It has been suggested that endogenous opioids releassed during the stress may attenuate gastric ulcer lesions. The aim of this study was to investigate effects of morpine on development of ulcer lessions, pathohistological alterations and antioxidative status in stomach of the rats exposed to indomethacin induced stress. Research was performed on adult, male Wistar rats weighting 200-230 g. Indomethacin stress was induced by intragastric administration of indomethacin at a dose of 20 mg/kg b.w. 6 hours before sacrificing. Morphine was applied intraperitoneally, in the doses of 10 mg/kg b.w. 15 minutes before indomethacin induced stress. The size of lesions in the form of petechiae and erosion, is expressed as the total surface of changes (mm2), i.e. ulcer index (UI). The pathohistological samples were analyzed by Leica DML S2 light microscope, and specific changes were photodocumented with Canon Power Shot S70 digital camera. In the homogenate of the stomach, the activity of catalase (CAT), glutathione peroxidase (GSHPx), glutathione reductase (GR) and xanthine oxidase (XOD) were measured, as well as the reduced glutathione content (GSH) and the lipid peroxidation intensity (Lpx). Morphine significantly reduced the ulcer index (UI) in animals exposed to indomethacin stress and the presence of large amounts of mucus in the stomach mucosal was established histopathologically. The use of morphine in the pretreatmant of indomethacin induced stress statistically significantly reduced the activity of all enzymes in the stomach compared to the control group, and this activity of catalase (CAT), glutathione peroxidase (GSHPx) and glutathione reductase (GR ), xanthine oxidase (XOD),as well as the lipid peroxidation intensity (Lpx), while the reduced glutathione content remained unchanged. Gastroprotective morphine activity in animals exposed to indomethacin induced stress is most likely a ... Article in Journal/Newspaper DML Directory of Open Access Journals: DOAJ Articles |
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Directory of Open Access Journals: DOAJ Articles |
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topic |
morphine indomethacin stress ulcer oxidative stress antioxidative status Medicine R |
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morphine indomethacin stress ulcer oxidative stress antioxidative status Medicine R Rašić Julijana Hudomal-Janićijević Snežana Stanojević-Ristić Zorica Kisić Bojana Stević Snežana Vitković Leonida Mijović Milica The effect of morphine on development of ulcer lesions of the rats exposed to indomethacin induced stress |
topic_facet |
morphine indomethacin stress ulcer oxidative stress antioxidative status Medicine R |
description |
Oxidative stress plays an important role in development of ulcer lessions of the rats exposed to indomethacin induced stress. It has been suggested that endogenous opioids releassed during the stress may attenuate gastric ulcer lesions. The aim of this study was to investigate effects of morpine on development of ulcer lessions, pathohistological alterations and antioxidative status in stomach of the rats exposed to indomethacin induced stress. Research was performed on adult, male Wistar rats weighting 200-230 g. Indomethacin stress was induced by intragastric administration of indomethacin at a dose of 20 mg/kg b.w. 6 hours before sacrificing. Morphine was applied intraperitoneally, in the doses of 10 mg/kg b.w. 15 minutes before indomethacin induced stress. The size of lesions in the form of petechiae and erosion, is expressed as the total surface of changes (mm2), i.e. ulcer index (UI). The pathohistological samples were analyzed by Leica DML S2 light microscope, and specific changes were photodocumented with Canon Power Shot S70 digital camera. In the homogenate of the stomach, the activity of catalase (CAT), glutathione peroxidase (GSHPx), glutathione reductase (GR) and xanthine oxidase (XOD) were measured, as well as the reduced glutathione content (GSH) and the lipid peroxidation intensity (Lpx). Morphine significantly reduced the ulcer index (UI) in animals exposed to indomethacin stress and the presence of large amounts of mucus in the stomach mucosal was established histopathologically. The use of morphine in the pretreatmant of indomethacin induced stress statistically significantly reduced the activity of all enzymes in the stomach compared to the control group, and this activity of catalase (CAT), glutathione peroxidase (GSHPx) and glutathione reductase (GR ), xanthine oxidase (XOD),as well as the lipid peroxidation intensity (Lpx), while the reduced glutathione content remained unchanged. Gastroprotective morphine activity in animals exposed to indomethacin induced stress is most likely a ... |
format |
Article in Journal/Newspaper |
author |
Rašić Julijana Hudomal-Janićijević Snežana Stanojević-Ristić Zorica Kisić Bojana Stević Snežana Vitković Leonida Mijović Milica |
author_facet |
Rašić Julijana Hudomal-Janićijević Snežana Stanojević-Ristić Zorica Kisić Bojana Stević Snežana Vitković Leonida Mijović Milica |
author_sort |
Rašić Julijana |
title |
The effect of morphine on development of ulcer lesions of the rats exposed to indomethacin induced stress |
title_short |
The effect of morphine on development of ulcer lesions of the rats exposed to indomethacin induced stress |
title_full |
The effect of morphine on development of ulcer lesions of the rats exposed to indomethacin induced stress |
title_fullStr |
The effect of morphine on development of ulcer lesions of the rats exposed to indomethacin induced stress |
title_full_unstemmed |
The effect of morphine on development of ulcer lesions of the rats exposed to indomethacin induced stress |
title_sort |
effect of morphine on development of ulcer lesions of the rats exposed to indomethacin induced stress |
publisher |
Medicinski fakultet Priština, Društvo lekara Kosova i Metohije Srpskog lekarskog društva |
publishDate |
2018 |
url |
https://doaj.org/article/62c06e6a648448b3b36410926064fdc7 |
genre |
DML |
genre_facet |
DML |
op_source |
Praxis Medica, Vol 47, Iss 1-2, Pp 1-8 (2018) |
op_relation |
https://scindeks-clanci.ceon.rs/data/pdf/0350-8773/2018/0350-87731802001R.pdf https://doaj.org/toc/0350-8773 https://doaj.org/toc/2560-3310 0350-8773 2560-3310 https://doaj.org/article/62c06e6a648448b3b36410926064fdc7 |
_version_ |
1766397734810550272 |