Infection of Borna disease virus in healthy animals in northern China
Borna disease virus (BDV) is a non-segmented, single negative stranded RNA virus. It is highly neurotropic, but the infection is not cytopathic to the infected cells. BDV is widely infected in vertebrates, causing abnormal behavior and multiple pathological changes. Horses and sheep are the main nat...
Published in: | Asian Pacific Journal of Tropical Medicine |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2018
|
Subjects: | |
Online Access: | https://doi.org/10.4103/1995-7645.243097 https://doaj.org/article/62afbfd856ad41839e9ec52a4bf52e58 |
id |
ftdoajarticles:oai:doaj.org/article:62afbfd856ad41839e9ec52a4bf52e58 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:62afbfd856ad41839e9ec52a4bf52e58 2023-05-15T15:15:17+02:00 Infection of Borna disease virus in healthy animals in northern China Ai-xia Zhai Ai-mei Li Wu-qi Song Wen-ping Kao Jun Qian Yu-jun Li Qing-meng Zhang Le-le Cui Tong Bu Han-xu Zhang Feng-min Zhang 2018-01-01T00:00:00Z https://doi.org/10.4103/1995-7645.243097 https://doaj.org/article/62afbfd856ad41839e9ec52a4bf52e58 EN eng Wolters Kluwer Medknow Publications http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=13;spage=30;epage=30;aulast=Zhai https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.243097 https://doaj.org/article/62afbfd856ad41839e9ec52a4bf52e58 Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 13, Pp 30-30 (2018) Borna disease virus Horse Sheep Northern China Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.4103/1995-7645.243097 2022-12-31T06:37:33Z Borna disease virus (BDV) is a non-segmented, single negative stranded RNA virus. It is highly neurotropic, but the infection is not cytopathic to the infected cells. BDV is widely infected in vertebrates, causing abnormal behavior and multiple pathological changes. Horses and sheep are the main natural hosts of BDV. Borna disease (BD) is characterized by progressive mononuclear encephalomyelitis. The BD clinical manifestations include ataxia, depression, rotation movement, mandatory standing, hitting objects with body and paralysis, and they usually last for 1-3 weeks. BDV infection causes a significant health problem in animals and economic loss for the society. However, BDV natural infection frequency in the raised animals in northern China, is poorly understood, but is required for understanding BDV infection epidemiology. BDV infection can be diagnosed through detecting viral proteins, antibodies and nucleic acid. The fluorescence based quantitative PCR is commonly used for BDV diagnosis because of the superior sensitivity. The assays for detecting plasma BDV specific antibodies include immunofluorescence, Western Blot and ELISA. Western Blot offers comparatively high specificity, which can detect different antibodies on the same blot/strip, and is often used as the confirmatory assay. This study investigated the infection prevalence of BDV in the meadow that raised horses and sheep in northern China. Blood samples were collected from a herd of healthy horses and sheep that were meadow raised in Hailin City, Heilongjiang Province, China. The uncoagulated blood samples were used to isolate peripheral lymphocytes and plasma. We selected Mongolian horses which are stocky, with relatively short but strong legs and a large head. The selected Han sheep is relatively small, has short tail and weighs 35-45 kg on average. BDV p40 mRNA was detected by Taqman based quantitative RT-PCR in lymphocytes of horses and sheep. BDV His-phosphoprotein (P) and His-nucleoprotein (N) fusion proteins were isolated with Histidine ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Medicine 11 13 30 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Borna disease virus Horse Sheep Northern China Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Borna disease virus Horse Sheep Northern China Arctic medicine. Tropical medicine RC955-962 Ai-xia Zhai Ai-mei Li Wu-qi Song Wen-ping Kao Jun Qian Yu-jun Li Qing-meng Zhang Le-le Cui Tong Bu Han-xu Zhang Feng-min Zhang Infection of Borna disease virus in healthy animals in northern China |
topic_facet |
Borna disease virus Horse Sheep Northern China Arctic medicine. Tropical medicine RC955-962 |
description |
Borna disease virus (BDV) is a non-segmented, single negative stranded RNA virus. It is highly neurotropic, but the infection is not cytopathic to the infected cells. BDV is widely infected in vertebrates, causing abnormal behavior and multiple pathological changes. Horses and sheep are the main natural hosts of BDV. Borna disease (BD) is characterized by progressive mononuclear encephalomyelitis. The BD clinical manifestations include ataxia, depression, rotation movement, mandatory standing, hitting objects with body and paralysis, and they usually last for 1-3 weeks. BDV infection causes a significant health problem in animals and economic loss for the society. However, BDV natural infection frequency in the raised animals in northern China, is poorly understood, but is required for understanding BDV infection epidemiology. BDV infection can be diagnosed through detecting viral proteins, antibodies and nucleic acid. The fluorescence based quantitative PCR is commonly used for BDV diagnosis because of the superior sensitivity. The assays for detecting plasma BDV specific antibodies include immunofluorescence, Western Blot and ELISA. Western Blot offers comparatively high specificity, which can detect different antibodies on the same blot/strip, and is often used as the confirmatory assay. This study investigated the infection prevalence of BDV in the meadow that raised horses and sheep in northern China. Blood samples were collected from a herd of healthy horses and sheep that were meadow raised in Hailin City, Heilongjiang Province, China. The uncoagulated blood samples were used to isolate peripheral lymphocytes and plasma. We selected Mongolian horses which are stocky, with relatively short but strong legs and a large head. The selected Han sheep is relatively small, has short tail and weighs 35-45 kg on average. BDV p40 mRNA was detected by Taqman based quantitative RT-PCR in lymphocytes of horses and sheep. BDV His-phosphoprotein (P) and His-nucleoprotein (N) fusion proteins were isolated with Histidine ... |
format |
Article in Journal/Newspaper |
author |
Ai-xia Zhai Ai-mei Li Wu-qi Song Wen-ping Kao Jun Qian Yu-jun Li Qing-meng Zhang Le-le Cui Tong Bu Han-xu Zhang Feng-min Zhang |
author_facet |
Ai-xia Zhai Ai-mei Li Wu-qi Song Wen-ping Kao Jun Qian Yu-jun Li Qing-meng Zhang Le-le Cui Tong Bu Han-xu Zhang Feng-min Zhang |
author_sort |
Ai-xia Zhai |
title |
Infection of Borna disease virus in healthy animals in northern China |
title_short |
Infection of Borna disease virus in healthy animals in northern China |
title_full |
Infection of Borna disease virus in healthy animals in northern China |
title_fullStr |
Infection of Borna disease virus in healthy animals in northern China |
title_full_unstemmed |
Infection of Borna disease virus in healthy animals in northern China |
title_sort |
infection of borna disease virus in healthy animals in northern china |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/1995-7645.243097 https://doaj.org/article/62afbfd856ad41839e9ec52a4bf52e58 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 13, Pp 30-30 (2018) |
op_relation |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=13;spage=30;epage=30;aulast=Zhai https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.243097 https://doaj.org/article/62afbfd856ad41839e9ec52a4bf52e58 |
op_doi |
https://doi.org/10.4103/1995-7645.243097 |
container_title |
Asian Pacific Journal of Tropical Medicine |
container_volume |
11 |
container_issue |
13 |
container_start_page |
30 |
_version_ |
1766345655625711616 |