Serological, electrophoretic and biological properties of Fasciola hepatica antigens Propiedades serologicas, electroforeticas y biologicas de antigenos de Fasciola hepatica
Fasciola hepatica somatic antigen, its partially purified fractions and excretion-secretion products were investigated as to serological, electrophoretic and biological properties. In a Sephadex G-100 column (SG-100), Fasciola hepatica total antigen (FhTA) gave 5 fractions, and SDS-PAGE analysis sho...
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Universidade de São Paulo
1992
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ftdoajarticles:oai:doaj.org/article:62634817f7174fe9881334a39c28992a 2023-05-15T15:18:08+02:00 Serological, electrophoretic and biological properties of Fasciola hepatica antigens Propiedades serologicas, electroforeticas y biologicas de antigenos de Fasciola hepatica Laura A. Cervi Héctor Rubinstein Diana T. Masih 1992-12-01T00:00:00Z https://doi.org/10.1590/S0036-46651992000600005 https://doaj.org/article/62634817f7174fe9881334a39c28992a EN PT eng por Universidade de São Paulo http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651992000600005 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651992000600005 0036-4665 1678-9946 https://doaj.org/article/62634817f7174fe9881334a39c28992a Revista do Instituto de Medicina Tropical de São Paulo, Vol 34, Iss 6, Pp 517-525 (1992) Fasciola hepatica Immune response Excretor-secretor antigen Antigens characterization Arctic medicine. Tropical medicine RC955-962 article 1992 ftdoajarticles https://doi.org/10.1590/S0036-46651992000600005 2023-01-08T01:26:58Z Fasciola hepatica somatic antigen, its partially purified fractions and excretion-secretion products were investigated as to serological, electrophoretic and biological properties. In a Sephadex G-100 column (SG-100), Fasciola hepatica total antigen (FhTA) gave 5 fractions, and SDS-PAGE analysis showed they were glycoproteins ranging from 14 to 94 kDa molecular weight (MW). When these fractions were analyzed by enzyme linked immunotransfer blot (EITB) and immunodiffusion in gel (ID) with serum from immunized rats with FhTA, the presence of different antigenic components was revealed. In the SDS-PAGE of excretor-secretor antigen (ESA), it was possible to observe peptides from 12 to 22 kDa, which were also present in FhTA. When the FhTA, its fractions and the ESA were analyzed by EITB with the immune rat serum (IRS), it was observed that only some fractions of the SG-100 shared antigens with the FhTA and ESA. Moreover, DTH and ITH responses were studied in FhTA immunized rats challenged with these different antigen components, revealing that the protein/carbohydrate ratio is important for inducing DTH response. The ESA was the most active component in the DTH and ITH response. Se realizó la purificación parcial de un antigeno somático de Fasciola hepatica y se obtuveron los productos de excreción-secreción. Por filtración del antigeno total de Fasciola hepatica en Sephadex G-100 (SG-100), se obtuvieron 5 fracciones las que al ser analizadas por electroforesis en geles de poliacrilamida, demostraron estar constituidas por glicoproteínas con un rango de peso molecular (PM) entre 14 y 94 kDa. Cuando estas fracciones fueron analizadas por immunoblot e inmunodifusión en geles de agar frente al suero de ratas inmunizadas con el homogenato total se reveló la presencia de diferentes componentes antigénicos. Usando un antígeno excretor-secretor fue posible observar en el perfil electroforético la presencia de péptidos con un PM entre 12 y 22 kDa, los cuales también estan presentes en el antígeno total. Cuando el antígeno ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 34 6 517 525 |
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topic |
Fasciola hepatica Immune response Excretor-secretor antigen Antigens characterization Arctic medicine. Tropical medicine RC955-962 |
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Fasciola hepatica Immune response Excretor-secretor antigen Antigens characterization Arctic medicine. Tropical medicine RC955-962 Laura A. Cervi Héctor Rubinstein Diana T. Masih Serological, electrophoretic and biological properties of Fasciola hepatica antigens Propiedades serologicas, electroforeticas y biologicas de antigenos de Fasciola hepatica |
topic_facet |
Fasciola hepatica Immune response Excretor-secretor antigen Antigens characterization Arctic medicine. Tropical medicine RC955-962 |
description |
Fasciola hepatica somatic antigen, its partially purified fractions and excretion-secretion products were investigated as to serological, electrophoretic and biological properties. In a Sephadex G-100 column (SG-100), Fasciola hepatica total antigen (FhTA) gave 5 fractions, and SDS-PAGE analysis showed they were glycoproteins ranging from 14 to 94 kDa molecular weight (MW). When these fractions were analyzed by enzyme linked immunotransfer blot (EITB) and immunodiffusion in gel (ID) with serum from immunized rats with FhTA, the presence of different antigenic components was revealed. In the SDS-PAGE of excretor-secretor antigen (ESA), it was possible to observe peptides from 12 to 22 kDa, which were also present in FhTA. When the FhTA, its fractions and the ESA were analyzed by EITB with the immune rat serum (IRS), it was observed that only some fractions of the SG-100 shared antigens with the FhTA and ESA. Moreover, DTH and ITH responses were studied in FhTA immunized rats challenged with these different antigen components, revealing that the protein/carbohydrate ratio is important for inducing DTH response. The ESA was the most active component in the DTH and ITH response. Se realizó la purificación parcial de un antigeno somático de Fasciola hepatica y se obtuveron los productos de excreción-secreción. Por filtración del antigeno total de Fasciola hepatica en Sephadex G-100 (SG-100), se obtuvieron 5 fracciones las que al ser analizadas por electroforesis en geles de poliacrilamida, demostraron estar constituidas por glicoproteínas con un rango de peso molecular (PM) entre 14 y 94 kDa. Cuando estas fracciones fueron analizadas por immunoblot e inmunodifusión en geles de agar frente al suero de ratas inmunizadas con el homogenato total se reveló la presencia de diferentes componentes antigénicos. Usando un antígeno excretor-secretor fue posible observar en el perfil electroforético la presencia de péptidos con un PM entre 12 y 22 kDa, los cuales también estan presentes en el antígeno total. Cuando el antígeno ... |
format |
Article in Journal/Newspaper |
author |
Laura A. Cervi Héctor Rubinstein Diana T. Masih |
author_facet |
Laura A. Cervi Héctor Rubinstein Diana T. Masih |
author_sort |
Laura A. Cervi |
title |
Serological, electrophoretic and biological properties of Fasciola hepatica antigens Propiedades serologicas, electroforeticas y biologicas de antigenos de Fasciola hepatica |
title_short |
Serological, electrophoretic and biological properties of Fasciola hepatica antigens Propiedades serologicas, electroforeticas y biologicas de antigenos de Fasciola hepatica |
title_full |
Serological, electrophoretic and biological properties of Fasciola hepatica antigens Propiedades serologicas, electroforeticas y biologicas de antigenos de Fasciola hepatica |
title_fullStr |
Serological, electrophoretic and biological properties of Fasciola hepatica antigens Propiedades serologicas, electroforeticas y biologicas de antigenos de Fasciola hepatica |
title_full_unstemmed |
Serological, electrophoretic and biological properties of Fasciola hepatica antigens Propiedades serologicas, electroforeticas y biologicas de antigenos de Fasciola hepatica |
title_sort |
serological, electrophoretic and biological properties of fasciola hepatica antigens propiedades serologicas, electroforeticas y biologicas de antigenos de fasciola hepatica |
publisher |
Universidade de São Paulo |
publishDate |
1992 |
url |
https://doi.org/10.1590/S0036-46651992000600005 https://doaj.org/article/62634817f7174fe9881334a39c28992a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 34, Iss 6, Pp 517-525 (1992) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651992000600005 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651992000600005 0036-4665 1678-9946 https://doaj.org/article/62634817f7174fe9881334a39c28992a |
op_doi |
https://doi.org/10.1590/S0036-46651992000600005 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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34 |
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