Phylogeographic diversity and hybrid zone of Hantaan orthohantavirus collected in Gangwon Province, Republic of Korea.
Background Hantaan orthohantavirus (Hantaan virus, HTNV), harbored by Apodemus agrarius (the striped field mouse), causes hemorrhagic fever with renal syndrome (HFRS) in humans. Viral genome-based surveillance at new expansion sites to identify HFRS risks plays a critical role in tracking the infect...
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ftdoajarticles:oai:doaj.org/article:623c3cbf5be14ecf93f1e960ece720e0 2023-05-15T15:14:11+02:00 Phylogeographic diversity and hybrid zone of Hantaan orthohantavirus collected in Gangwon Province, Republic of Korea. Geum-Young Lee Won-Keun Kim Kyungmin Park Seung-Ho Lee Jusun Hwang Jin Sun No Seungchan Cho Daesang Lee Dong-Hyun Song Se Hun Gu Man-Seong Park Seong Tae Jeong Young-Su Kim Jin-Won Song 2020-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008714 https://doaj.org/article/623c3cbf5be14ecf93f1e960ece720e0 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008714 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008714 https://doaj.org/article/623c3cbf5be14ecf93f1e960ece720e0 PLoS Neglected Tropical Diseases, Vol 14, Iss 10, p e0008714 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008714 2022-12-31T13:13:07Z Background Hantaan orthohantavirus (Hantaan virus, HTNV), harbored by Apodemus agrarius (the striped field mouse), causes hemorrhagic fever with renal syndrome (HFRS) in humans. Viral genome-based surveillance at new expansion sites to identify HFRS risks plays a critical role in tracking the infection source of orthohantavirus outbreak. In the Republic of Korea (ROK), most studies demonstrated the serological prevalence and genetic diversity of orthohantaviruses collected from HFRS patients or rodents in Gyeonggi Province. Gangwon Province is a HFRS-endemic area with a high incidence of patients and prevalence of infected rodents, ROK. However, the continued epidemiology and surveillance of orthohantavirus remain to be investigated. Methodology/principal findings Whole-genome sequencing of HTNV was accomplished in small mammals collected in Gangwon Province during 2015-2018 by multiplex PCR-based next-generation sequencing. To elucidate the geographic distribution and molecular diversity of viruses, we conducted phylogenetic analyses of HTNV tripartite genomes. We inferred the hybrid zone using cline analysis to estimate the geographic contact between two different HTNV lineages in the ROK. The graph incompatibility based reassortment finder performed reassortment analysis. A total of 12 HTNV genome sequences were completely obtained from A. agrarius newly collected in Gangwon Province. The phylogenetic and cline analyses demonstrated the genetic diversity and hybrid zone of HTNV in the ROK. Genetic exchange analysis suggested the possibility of reassortments in Cheorwon-gun, a highly HFRS-endemic area. Conclusions/significance The prevalence and distribution of HTNV in HFRS-endemic areas of Gangwon Province enhanced the phylogeographic map for orthohantavirus outbreak monitoring in ROK. This study revealed the hybrid zone reflecting the genetic diversity and evolutionary dynamics of HTNV circulating in Gangwon Province. The results arise awareness of rodent-borne orthohantavirus diseases for physicians in the ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 10 e0008714 |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Geum-Young Lee Won-Keun Kim Kyungmin Park Seung-Ho Lee Jusun Hwang Jin Sun No Seungchan Cho Daesang Lee Dong-Hyun Song Se Hun Gu Man-Seong Park Seong Tae Jeong Young-Su Kim Jin-Won Song Phylogeographic diversity and hybrid zone of Hantaan orthohantavirus collected in Gangwon Province, Republic of Korea. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Hantaan orthohantavirus (Hantaan virus, HTNV), harbored by Apodemus agrarius (the striped field mouse), causes hemorrhagic fever with renal syndrome (HFRS) in humans. Viral genome-based surveillance at new expansion sites to identify HFRS risks plays a critical role in tracking the infection source of orthohantavirus outbreak. In the Republic of Korea (ROK), most studies demonstrated the serological prevalence and genetic diversity of orthohantaviruses collected from HFRS patients or rodents in Gyeonggi Province. Gangwon Province is a HFRS-endemic area with a high incidence of patients and prevalence of infected rodents, ROK. However, the continued epidemiology and surveillance of orthohantavirus remain to be investigated. Methodology/principal findings Whole-genome sequencing of HTNV was accomplished in small mammals collected in Gangwon Province during 2015-2018 by multiplex PCR-based next-generation sequencing. To elucidate the geographic distribution and molecular diversity of viruses, we conducted phylogenetic analyses of HTNV tripartite genomes. We inferred the hybrid zone using cline analysis to estimate the geographic contact between two different HTNV lineages in the ROK. The graph incompatibility based reassortment finder performed reassortment analysis. A total of 12 HTNV genome sequences were completely obtained from A. agrarius newly collected in Gangwon Province. The phylogenetic and cline analyses demonstrated the genetic diversity and hybrid zone of HTNV in the ROK. Genetic exchange analysis suggested the possibility of reassortments in Cheorwon-gun, a highly HFRS-endemic area. Conclusions/significance The prevalence and distribution of HTNV in HFRS-endemic areas of Gangwon Province enhanced the phylogeographic map for orthohantavirus outbreak monitoring in ROK. This study revealed the hybrid zone reflecting the genetic diversity and evolutionary dynamics of HTNV circulating in Gangwon Province. The results arise awareness of rodent-borne orthohantavirus diseases for physicians in the ... |
format |
Article in Journal/Newspaper |
author |
Geum-Young Lee Won-Keun Kim Kyungmin Park Seung-Ho Lee Jusun Hwang Jin Sun No Seungchan Cho Daesang Lee Dong-Hyun Song Se Hun Gu Man-Seong Park Seong Tae Jeong Young-Su Kim Jin-Won Song |
author_facet |
Geum-Young Lee Won-Keun Kim Kyungmin Park Seung-Ho Lee Jusun Hwang Jin Sun No Seungchan Cho Daesang Lee Dong-Hyun Song Se Hun Gu Man-Seong Park Seong Tae Jeong Young-Su Kim Jin-Won Song |
author_sort |
Geum-Young Lee |
title |
Phylogeographic diversity and hybrid zone of Hantaan orthohantavirus collected in Gangwon Province, Republic of Korea. |
title_short |
Phylogeographic diversity and hybrid zone of Hantaan orthohantavirus collected in Gangwon Province, Republic of Korea. |
title_full |
Phylogeographic diversity and hybrid zone of Hantaan orthohantavirus collected in Gangwon Province, Republic of Korea. |
title_fullStr |
Phylogeographic diversity and hybrid zone of Hantaan orthohantavirus collected in Gangwon Province, Republic of Korea. |
title_full_unstemmed |
Phylogeographic diversity and hybrid zone of Hantaan orthohantavirus collected in Gangwon Province, Republic of Korea. |
title_sort |
phylogeographic diversity and hybrid zone of hantaan orthohantavirus collected in gangwon province, republic of korea. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008714 https://doaj.org/article/623c3cbf5be14ecf93f1e960ece720e0 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 10, p e0008714 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008714 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008714 https://doaj.org/article/623c3cbf5be14ecf93f1e960ece720e0 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008714 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
10 |
container_start_page |
e0008714 |
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1766344672542720000 |