Monitoring Fungal Communities With the Global Spore Sampling Project
The kingdom Fungi is a megadiverse group represented in all ecosystem types. The global diversity and distribution of fungal taxa are poorly known, in part due to the limitations related to traditional fruit-body survey methods. These previous hurdles are now being overcome by rapidly developing DNA...
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ftdoajarticles:oai:doaj.org/article:6232c510cbcd4b489e439560e7fc7b1a 2023-05-15T16:29:48+02:00 Monitoring Fungal Communities With the Global Spore Sampling Project Otso Ovaskainen Nerea Abrego Panu Somervuo Isabella Palorinne Bess Hardwick Juha-Matti Pitkänen Nigel R. Andrew Pascal A. Niklaus Niels Martin Schmidt Sebastian Seibold Juliane Vogt Evgeny V. Zakharov Paul D. N. Hebert Tomas Roslin Natalia V. Ivanova 2020-01-01T00:00:00Z https://doi.org/10.3389/fevo.2019.00511 https://doaj.org/article/6232c510cbcd4b489e439560e7fc7b1a EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fevo.2019.00511/full https://doaj.org/toc/2296-701X 2296-701X doi:10.3389/fevo.2019.00511 https://doaj.org/article/6232c510cbcd4b489e439560e7fc7b1a Frontiers in Ecology and Evolution, Vol 7 (2020) biomonitoring cyclone sampler environmental DNA fungi global diversity Evolution QH359-425 Ecology QH540-549.5 article 2020 ftdoajarticles https://doi.org/10.3389/fevo.2019.00511 2022-12-31T00:15:46Z The kingdom Fungi is a megadiverse group represented in all ecosystem types. The global diversity and distribution of fungal taxa are poorly known, in part due to the limitations related to traditional fruit-body survey methods. These previous hurdles are now being overcome by rapidly developing DNA-based surveys. Past fungal DNA surveys have predominantly examined soil samples, which capture high species diversity but represent only the local soil community. Recent work has shown that DNA samples collected from the air with cyclone samplers provide information on fungal diversity at the scale of some tens of kilometers around the sampling location. To test the feasibility of air sampling for investigating global patterns of fungal diversity, we established a new initiative called the Global Spore Sampling Project (GSSP). The GSSP currently involves 50 sampling locations distributed on all continents, with each location collecting two 24-h samples per week. Here we describe the GSSP methodology, including the sampling, DNA extraction and sequencing protocols, and the bioinformatics pipeline. We further report results based on 75 pilot samples from five locations, of which three in Europe, one in Australia, and one in Greenland. The results show highly consistent patterns, suggesting that GSSP holds much promise for systematic global fungal monitoring. The GSSP provides highly standardized sampling across space and time, enabling much-improved estimation of total fungal diversity, the global distribution of different fungal groups, fungal fruiting phenology, and the extent of long-distance dispersal in fungi. Article in Journal/Newspaper Greenland Directory of Open Access Journals: DOAJ Articles Greenland Frontiers in Ecology and Evolution 7 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
biomonitoring cyclone sampler environmental DNA fungi global diversity Evolution QH359-425 Ecology QH540-549.5 |
spellingShingle |
biomonitoring cyclone sampler environmental DNA fungi global diversity Evolution QH359-425 Ecology QH540-549.5 Otso Ovaskainen Nerea Abrego Panu Somervuo Isabella Palorinne Bess Hardwick Juha-Matti Pitkänen Nigel R. Andrew Pascal A. Niklaus Niels Martin Schmidt Sebastian Seibold Juliane Vogt Evgeny V. Zakharov Paul D. N. Hebert Tomas Roslin Natalia V. Ivanova Monitoring Fungal Communities With the Global Spore Sampling Project |
topic_facet |
biomonitoring cyclone sampler environmental DNA fungi global diversity Evolution QH359-425 Ecology QH540-549.5 |
description |
The kingdom Fungi is a megadiverse group represented in all ecosystem types. The global diversity and distribution of fungal taxa are poorly known, in part due to the limitations related to traditional fruit-body survey methods. These previous hurdles are now being overcome by rapidly developing DNA-based surveys. Past fungal DNA surveys have predominantly examined soil samples, which capture high species diversity but represent only the local soil community. Recent work has shown that DNA samples collected from the air with cyclone samplers provide information on fungal diversity at the scale of some tens of kilometers around the sampling location. To test the feasibility of air sampling for investigating global patterns of fungal diversity, we established a new initiative called the Global Spore Sampling Project (GSSP). The GSSP currently involves 50 sampling locations distributed on all continents, with each location collecting two 24-h samples per week. Here we describe the GSSP methodology, including the sampling, DNA extraction and sequencing protocols, and the bioinformatics pipeline. We further report results based on 75 pilot samples from five locations, of which three in Europe, one in Australia, and one in Greenland. The results show highly consistent patterns, suggesting that GSSP holds much promise for systematic global fungal monitoring. The GSSP provides highly standardized sampling across space and time, enabling much-improved estimation of total fungal diversity, the global distribution of different fungal groups, fungal fruiting phenology, and the extent of long-distance dispersal in fungi. |
format |
Article in Journal/Newspaper |
author |
Otso Ovaskainen Nerea Abrego Panu Somervuo Isabella Palorinne Bess Hardwick Juha-Matti Pitkänen Nigel R. Andrew Pascal A. Niklaus Niels Martin Schmidt Sebastian Seibold Juliane Vogt Evgeny V. Zakharov Paul D. N. Hebert Tomas Roslin Natalia V. Ivanova |
author_facet |
Otso Ovaskainen Nerea Abrego Panu Somervuo Isabella Palorinne Bess Hardwick Juha-Matti Pitkänen Nigel R. Andrew Pascal A. Niklaus Niels Martin Schmidt Sebastian Seibold Juliane Vogt Evgeny V. Zakharov Paul D. N. Hebert Tomas Roslin Natalia V. Ivanova |
author_sort |
Otso Ovaskainen |
title |
Monitoring Fungal Communities With the Global Spore Sampling Project |
title_short |
Monitoring Fungal Communities With the Global Spore Sampling Project |
title_full |
Monitoring Fungal Communities With the Global Spore Sampling Project |
title_fullStr |
Monitoring Fungal Communities With the Global Spore Sampling Project |
title_full_unstemmed |
Monitoring Fungal Communities With the Global Spore Sampling Project |
title_sort |
monitoring fungal communities with the global spore sampling project |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
https://doi.org/10.3389/fevo.2019.00511 https://doaj.org/article/6232c510cbcd4b489e439560e7fc7b1a |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Greenland |
genre_facet |
Greenland |
op_source |
Frontiers in Ecology and Evolution, Vol 7 (2020) |
op_relation |
https://www.frontiersin.org/article/10.3389/fevo.2019.00511/full https://doaj.org/toc/2296-701X 2296-701X doi:10.3389/fevo.2019.00511 https://doaj.org/article/6232c510cbcd4b489e439560e7fc7b1a |
op_doi |
https://doi.org/10.3389/fevo.2019.00511 |
container_title |
Frontiers in Ecology and Evolution |
container_volume |
7 |
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1766019519270092800 |