Genomic Insight Into the Predominance of Candidate Phylum Atribacteria JS1 Lineage in Marine Sediments
Candidate phylum Atribacteria JS1 lineage is one of the predominant bacterial groups in anoxic subseafloor sediments, especially in organic-rich or gas hydrate-containing sediments. However, due to the lack of axenic culture representatives, metabolic potential and biogeochemical roles of this phylu...
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ftdoajarticles:oai:doaj.org/article:61fd13518ff94b4faf47642440e5a38d 2023-05-15T13:50:29+02:00 Genomic Insight Into the Predominance of Candidate Phylum Atribacteria JS1 Lineage in Marine Sediments Yung Mi Lee Kyuin Hwang Jae Il Lee Mincheol Kim Chung Yeon Hwang Hyun-Ju Noh Hakkyum Choi Hong Kum Lee Jongsik Chun Soon Gyu Hong Seung Chul Shin 2018-11-01T00:00:00Z https://doi.org/10.3389/fmicb.2018.02909 https://doaj.org/article/61fd13518ff94b4faf47642440e5a38d EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fmicb.2018.02909/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2018.02909 https://doaj.org/article/61fd13518ff94b4faf47642440e5a38d Frontiers in Microbiology, Vol 9 (2018) candidate division Atribacteria JS1 single-cell genomics Antarctica Ross Sea Microbiology QR1-502 article 2018 ftdoajarticles https://doi.org/10.3389/fmicb.2018.02909 2022-12-31T11:54:54Z Candidate phylum Atribacteria JS1 lineage is one of the predominant bacterial groups in anoxic subseafloor sediments, especially in organic-rich or gas hydrate-containing sediments. However, due to the lack of axenic culture representatives, metabolic potential and biogeochemical roles of this phylum have remained elusive. Here, we examined the microbial communities of marine sediments of the Ross Sea, Antarctica, and found candidate phylum Atribacteria JS1 lineage was the most abundant candidate phylum accounting for 9.8–40.8% of the bacterial communities with a single dominant operational taxonomic unit (OTU). To elucidate the metabolic potential and ecological function of this species, we applied a single-cell genomic approach and obtained 18 single-cell amplified genomes presumably from a single species that was consistent with the dominant OTU throughout the sediments. The composite genome constructed by co-assembly showed the highest genome completeness among available Atribacteria JS1 genomes. Metabolic reconstruction suggested fermentative potential using various substrates and syntrophic acetate oxidation coupled with hydrogen or formate scavenging methanogens. This metabolic potential supports the predominance of Atribacteria JS1 in anoxic environments expanding our knowledge of the ecological function of this uncultivated group. Article in Journal/Newspaper Antarc* Antarctica Ross Sea Directory of Open Access Journals: DOAJ Articles Ross Sea Frontiers in Microbiology 9 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
candidate division Atribacteria JS1 single-cell genomics Antarctica Ross Sea Microbiology QR1-502 |
spellingShingle |
candidate division Atribacteria JS1 single-cell genomics Antarctica Ross Sea Microbiology QR1-502 Yung Mi Lee Kyuin Hwang Jae Il Lee Mincheol Kim Chung Yeon Hwang Hyun-Ju Noh Hakkyum Choi Hong Kum Lee Jongsik Chun Soon Gyu Hong Seung Chul Shin Genomic Insight Into the Predominance of Candidate Phylum Atribacteria JS1 Lineage in Marine Sediments |
topic_facet |
candidate division Atribacteria JS1 single-cell genomics Antarctica Ross Sea Microbiology QR1-502 |
description |
Candidate phylum Atribacteria JS1 lineage is one of the predominant bacterial groups in anoxic subseafloor sediments, especially in organic-rich or gas hydrate-containing sediments. However, due to the lack of axenic culture representatives, metabolic potential and biogeochemical roles of this phylum have remained elusive. Here, we examined the microbial communities of marine sediments of the Ross Sea, Antarctica, and found candidate phylum Atribacteria JS1 lineage was the most abundant candidate phylum accounting for 9.8–40.8% of the bacterial communities with a single dominant operational taxonomic unit (OTU). To elucidate the metabolic potential and ecological function of this species, we applied a single-cell genomic approach and obtained 18 single-cell amplified genomes presumably from a single species that was consistent with the dominant OTU throughout the sediments. The composite genome constructed by co-assembly showed the highest genome completeness among available Atribacteria JS1 genomes. Metabolic reconstruction suggested fermentative potential using various substrates and syntrophic acetate oxidation coupled with hydrogen or formate scavenging methanogens. This metabolic potential supports the predominance of Atribacteria JS1 in anoxic environments expanding our knowledge of the ecological function of this uncultivated group. |
format |
Article in Journal/Newspaper |
author |
Yung Mi Lee Kyuin Hwang Jae Il Lee Mincheol Kim Chung Yeon Hwang Hyun-Ju Noh Hakkyum Choi Hong Kum Lee Jongsik Chun Soon Gyu Hong Seung Chul Shin |
author_facet |
Yung Mi Lee Kyuin Hwang Jae Il Lee Mincheol Kim Chung Yeon Hwang Hyun-Ju Noh Hakkyum Choi Hong Kum Lee Jongsik Chun Soon Gyu Hong Seung Chul Shin |
author_sort |
Yung Mi Lee |
title |
Genomic Insight Into the Predominance of Candidate Phylum Atribacteria JS1 Lineage in Marine Sediments |
title_short |
Genomic Insight Into the Predominance of Candidate Phylum Atribacteria JS1 Lineage in Marine Sediments |
title_full |
Genomic Insight Into the Predominance of Candidate Phylum Atribacteria JS1 Lineage in Marine Sediments |
title_fullStr |
Genomic Insight Into the Predominance of Candidate Phylum Atribacteria JS1 Lineage in Marine Sediments |
title_full_unstemmed |
Genomic Insight Into the Predominance of Candidate Phylum Atribacteria JS1 Lineage in Marine Sediments |
title_sort |
genomic insight into the predominance of candidate phylum atribacteria js1 lineage in marine sediments |
publisher |
Frontiers Media S.A. |
publishDate |
2018 |
url |
https://doi.org/10.3389/fmicb.2018.02909 https://doaj.org/article/61fd13518ff94b4faf47642440e5a38d |
geographic |
Ross Sea |
geographic_facet |
Ross Sea |
genre |
Antarc* Antarctica Ross Sea |
genre_facet |
Antarc* Antarctica Ross Sea |
op_source |
Frontiers in Microbiology, Vol 9 (2018) |
op_relation |
https://www.frontiersin.org/article/10.3389/fmicb.2018.02909/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2018.02909 https://doaj.org/article/61fd13518ff94b4faf47642440e5a38d |
op_doi |
https://doi.org/10.3389/fmicb.2018.02909 |
container_title |
Frontiers in Microbiology |
container_volume |
9 |
_version_ |
1766253527871520768 |