Synthesis of Bioactive Silver Nanoparticles by a Pseudomonas Strain Associated with the Antarctic Psychrophilic Protozoon Euplotes focardii
The synthesis of silver nanoparticles (AgNPs) by microorganisms recently gained a greater interest due to its potential to produce them in various sizes and morphologies. In this study, for AgNP biosynthesis, we used a new Pseudomonas strain isolated from a consortium associated with the Antarctic m...
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ftdoajarticles:oai:doaj.org/article:61e2dea22730469eb80414ea266a524f 2023-05-15T14:04:12+02:00 Synthesis of Bioactive Silver Nanoparticles by a Pseudomonas Strain Associated with the Antarctic Psychrophilic Protozoon Euplotes focardii Maria Sindhura John Joseph Amruthraj Nagoth Kesava Priyan Ramasamy Alessio Mancini Gabriele Giuli Antonino Natalello Patrizia Ballarini Cristina Miceli Sandra Pucciarelli 2020-01-01T00:00:00Z https://doi.org/10.3390/md18010038 https://doaj.org/article/61e2dea22730469eb80414ea266a524f EN eng MDPI AG https://www.mdpi.com/1660-3397/18/1/38 https://doaj.org/toc/1660-3397 1660-3397 doi:10.3390/md18010038 https://doaj.org/article/61e2dea22730469eb80414ea266a524f Marine Drugs, Vol 18, Iss 1, p 38 (2020) green synthesis biomaterials silver nitrate antibiotics nanotechnology Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.3390/md18010038 2022-12-30T23:31:59Z The synthesis of silver nanoparticles (AgNPs) by microorganisms recently gained a greater interest due to its potential to produce them in various sizes and morphologies. In this study, for AgNP biosynthesis, we used a new Pseudomonas strain isolated from a consortium associated with the Antarctic marine ciliate Euplotes focardii . After incubation of Pseudomonas cultures with 1 mM of AgNO 3 at 22 °C, we obtained AgNPs within 24 h. Scanning electron (SEM) and transmission electron microscopy (TEM) revealed spherical polydispersed AgNPs in the size range of 20−70 nm. The average size was approximately 50 nm. Energy dispersive X-ray spectroscopy (EDS) showed the presence of a high intensity absorption peak at 3 keV, a distinctive property of nanocrystalline silver products. Fourier transform infrared (FTIR) spectroscopy found the presence of a high amount of AgNP-stabilizing proteins and other secondary metabolites. X-ray diffraction (XRD) revealed a face-centred cubic (fcc) diffraction spectrum with a crystalline nature. A comparative study between the chemically synthesized and Pseudomonas AgNPs revealed a higher antibacterial activity of the latter against common nosocomial pathogen microorganisms, including Escherichia coli, Staphylococcus aureus and Candida albicans . This study reports an efficient, rapid synthesis of stable AgNPs by a new Pseudomonas strain with high antimicrobial activity. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Marine Drugs 18 1 38 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
green synthesis biomaterials silver nitrate antibiotics nanotechnology Biology (General) QH301-705.5 |
spellingShingle |
green synthesis biomaterials silver nitrate antibiotics nanotechnology Biology (General) QH301-705.5 Maria Sindhura John Joseph Amruthraj Nagoth Kesava Priyan Ramasamy Alessio Mancini Gabriele Giuli Antonino Natalello Patrizia Ballarini Cristina Miceli Sandra Pucciarelli Synthesis of Bioactive Silver Nanoparticles by a Pseudomonas Strain Associated with the Antarctic Psychrophilic Protozoon Euplotes focardii |
topic_facet |
green synthesis biomaterials silver nitrate antibiotics nanotechnology Biology (General) QH301-705.5 |
description |
The synthesis of silver nanoparticles (AgNPs) by microorganisms recently gained a greater interest due to its potential to produce them in various sizes and morphologies. In this study, for AgNP biosynthesis, we used a new Pseudomonas strain isolated from a consortium associated with the Antarctic marine ciliate Euplotes focardii . After incubation of Pseudomonas cultures with 1 mM of AgNO 3 at 22 °C, we obtained AgNPs within 24 h. Scanning electron (SEM) and transmission electron microscopy (TEM) revealed spherical polydispersed AgNPs in the size range of 20−70 nm. The average size was approximately 50 nm. Energy dispersive X-ray spectroscopy (EDS) showed the presence of a high intensity absorption peak at 3 keV, a distinctive property of nanocrystalline silver products. Fourier transform infrared (FTIR) spectroscopy found the presence of a high amount of AgNP-stabilizing proteins and other secondary metabolites. X-ray diffraction (XRD) revealed a face-centred cubic (fcc) diffraction spectrum with a crystalline nature. A comparative study between the chemically synthesized and Pseudomonas AgNPs revealed a higher antibacterial activity of the latter against common nosocomial pathogen microorganisms, including Escherichia coli, Staphylococcus aureus and Candida albicans . This study reports an efficient, rapid synthesis of stable AgNPs by a new Pseudomonas strain with high antimicrobial activity. |
format |
Article in Journal/Newspaper |
author |
Maria Sindhura John Joseph Amruthraj Nagoth Kesava Priyan Ramasamy Alessio Mancini Gabriele Giuli Antonino Natalello Patrizia Ballarini Cristina Miceli Sandra Pucciarelli |
author_facet |
Maria Sindhura John Joseph Amruthraj Nagoth Kesava Priyan Ramasamy Alessio Mancini Gabriele Giuli Antonino Natalello Patrizia Ballarini Cristina Miceli Sandra Pucciarelli |
author_sort |
Maria Sindhura John |
title |
Synthesis of Bioactive Silver Nanoparticles by a Pseudomonas Strain Associated with the Antarctic Psychrophilic Protozoon Euplotes focardii |
title_short |
Synthesis of Bioactive Silver Nanoparticles by a Pseudomonas Strain Associated with the Antarctic Psychrophilic Protozoon Euplotes focardii |
title_full |
Synthesis of Bioactive Silver Nanoparticles by a Pseudomonas Strain Associated with the Antarctic Psychrophilic Protozoon Euplotes focardii |
title_fullStr |
Synthesis of Bioactive Silver Nanoparticles by a Pseudomonas Strain Associated with the Antarctic Psychrophilic Protozoon Euplotes focardii |
title_full_unstemmed |
Synthesis of Bioactive Silver Nanoparticles by a Pseudomonas Strain Associated with the Antarctic Psychrophilic Protozoon Euplotes focardii |
title_sort |
synthesis of bioactive silver nanoparticles by a pseudomonas strain associated with the antarctic psychrophilic protozoon euplotes focardii |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/md18010038 https://doaj.org/article/61e2dea22730469eb80414ea266a524f |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Marine Drugs, Vol 18, Iss 1, p 38 (2020) |
op_relation |
https://www.mdpi.com/1660-3397/18/1/38 https://doaj.org/toc/1660-3397 1660-3397 doi:10.3390/md18010038 https://doaj.org/article/61e2dea22730469eb80414ea266a524f |
op_doi |
https://doi.org/10.3390/md18010038 |
container_title |
Marine Drugs |
container_volume |
18 |
container_issue |
1 |
container_start_page |
38 |
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1766275218992529408 |