First isolation of Aeromonas salmonicida subspecies salmonicida from diseased sea bass, Dicentrarchus labrax (L.), cultured in Spain
This work represents the first description of Aeromonas salmonicida subsp. salmonicida as causative agent of furunculosis in cultured sea bass, Dicentrarchus labrax (L.). Cumulative mortality in affected fish from two floating cages in the Mediterranean coast of Spain was 3.8%. Affected sea bass did...
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ftdoajarticles:oai:doaj.org/article:61d2a6a2ee2445c096f5228a497f1ff8 2023-05-15T18:41:14+02:00 First isolation of Aeromonas salmonicida subspecies salmonicida from diseased sea bass, Dicentrarchus labrax (L.), cultured in Spain Clara Fernández-Álvarez Daniel Gijón Mireya Álvarez Ysabel Santos 2016-11-01T00:00:00Z https://doi.org/10.1016/j.aqrep.2016.05.006 https://doaj.org/article/61d2a6a2ee2445c096f5228a497f1ff8 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2352513416300461 https://doaj.org/toc/2352-5134 2352-5134 doi:10.1016/j.aqrep.2016.05.006 https://doaj.org/article/61d2a6a2ee2445c096f5228a497f1ff8 Aquaculture Reports, Vol 4, Iss C, Pp 36-41 (2016) Sea bass Aeromonas salmonicida subsp. salmonicida PCR MALDI-TOF Fatty acids Aquaculture. Fisheries. Angling SH1-691 article 2016 ftdoajarticles https://doi.org/10.1016/j.aqrep.2016.05.006 2022-12-31T12:30:26Z This work represents the first description of Aeromonas salmonicida subsp. salmonicida as causative agent of furunculosis in cultured sea bass, Dicentrarchus labrax (L.). Cumulative mortality in affected fish from two floating cages in the Mediterranean coast of Spain was 3.8%. Affected sea bass did not show the typical external signs of furunculosis in the first stages of the disease, however, when the disease progressed, open ulcers appeared on the skin and muscle. Internally, splenomegaly was the only pathological sign observed. Samples from diseased fish were subjected to standardized assays for pathogens screening. Negative results were obtained for parasites and fish viruses. A Gram-stain-negative rod-shaped bacterium was observed in smears from liver, kidney and spleen of all analysed fish. Pure bacterial cultures were recovered from liver, kidney and spleen of all diseased fish sampled during the two different outbreaks. Bacteriological, serological, molecular and chemotaxonomic analysis allowed the identification of the causative agent of sea bass mortalities as Aeromonas salmonicida subsp. salmonicida. The bacterial strains were susceptible to most of antimicrobial agents usually employed in aquaculture except to oxytetracycline. Pathogenicity assays demonstrated that the isolated bacteria were virulent for sea bass, turbot and rainbow trout. Article in Journal/Newspaper Turbot Directory of Open Access Journals: DOAJ Articles Aquaculture Reports 4 36 41 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Sea bass Aeromonas salmonicida subsp. salmonicida PCR MALDI-TOF Fatty acids Aquaculture. Fisheries. Angling SH1-691 |
spellingShingle |
Sea bass Aeromonas salmonicida subsp. salmonicida PCR MALDI-TOF Fatty acids Aquaculture. Fisheries. Angling SH1-691 Clara Fernández-Álvarez Daniel Gijón Mireya Álvarez Ysabel Santos First isolation of Aeromonas salmonicida subspecies salmonicida from diseased sea bass, Dicentrarchus labrax (L.), cultured in Spain |
topic_facet |
Sea bass Aeromonas salmonicida subsp. salmonicida PCR MALDI-TOF Fatty acids Aquaculture. Fisheries. Angling SH1-691 |
description |
This work represents the first description of Aeromonas salmonicida subsp. salmonicida as causative agent of furunculosis in cultured sea bass, Dicentrarchus labrax (L.). Cumulative mortality in affected fish from two floating cages in the Mediterranean coast of Spain was 3.8%. Affected sea bass did not show the typical external signs of furunculosis in the first stages of the disease, however, when the disease progressed, open ulcers appeared on the skin and muscle. Internally, splenomegaly was the only pathological sign observed. Samples from diseased fish were subjected to standardized assays for pathogens screening. Negative results were obtained for parasites and fish viruses. A Gram-stain-negative rod-shaped bacterium was observed in smears from liver, kidney and spleen of all analysed fish. Pure bacterial cultures were recovered from liver, kidney and spleen of all diseased fish sampled during the two different outbreaks. Bacteriological, serological, molecular and chemotaxonomic analysis allowed the identification of the causative agent of sea bass mortalities as Aeromonas salmonicida subsp. salmonicida. The bacterial strains were susceptible to most of antimicrobial agents usually employed in aquaculture except to oxytetracycline. Pathogenicity assays demonstrated that the isolated bacteria were virulent for sea bass, turbot and rainbow trout. |
format |
Article in Journal/Newspaper |
author |
Clara Fernández-Álvarez Daniel Gijón Mireya Álvarez Ysabel Santos |
author_facet |
Clara Fernández-Álvarez Daniel Gijón Mireya Álvarez Ysabel Santos |
author_sort |
Clara Fernández-Álvarez |
title |
First isolation of Aeromonas salmonicida subspecies salmonicida from diseased sea bass, Dicentrarchus labrax (L.), cultured in Spain |
title_short |
First isolation of Aeromonas salmonicida subspecies salmonicida from diseased sea bass, Dicentrarchus labrax (L.), cultured in Spain |
title_full |
First isolation of Aeromonas salmonicida subspecies salmonicida from diseased sea bass, Dicentrarchus labrax (L.), cultured in Spain |
title_fullStr |
First isolation of Aeromonas salmonicida subspecies salmonicida from diseased sea bass, Dicentrarchus labrax (L.), cultured in Spain |
title_full_unstemmed |
First isolation of Aeromonas salmonicida subspecies salmonicida from diseased sea bass, Dicentrarchus labrax (L.), cultured in Spain |
title_sort |
first isolation of aeromonas salmonicida subspecies salmonicida from diseased sea bass, dicentrarchus labrax (l.), cultured in spain |
publisher |
Elsevier |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.aqrep.2016.05.006 https://doaj.org/article/61d2a6a2ee2445c096f5228a497f1ff8 |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
Aquaculture Reports, Vol 4, Iss C, Pp 36-41 (2016) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2352513416300461 https://doaj.org/toc/2352-5134 2352-5134 doi:10.1016/j.aqrep.2016.05.006 https://doaj.org/article/61d2a6a2ee2445c096f5228a497f1ff8 |
op_doi |
https://doi.org/10.1016/j.aqrep.2016.05.006 |
container_title |
Aquaculture Reports |
container_volume |
4 |
container_start_page |
36 |
op_container_end_page |
41 |
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1766230731971887104 |