Insights into an Optimization of Plasmodium vivax Sal-1 In Vitro Culture: The Aotus Primate Model.
Malaria is one of the most significant tropical diseases, and of the Plasmodium species that cause human malaria, P. vivax is the most geographically widespread. However, P. vivax remains a relatively neglected human parasite since research is typically limited to laboratories with direct access to...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2016
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0004870 https://doaj.org/article/61b1a42801ab490e9b0bfd29a94d21db |
id |
ftdoajarticles:oai:doaj.org/article:61b1a42801ab490e9b0bfd29a94d21db |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:61b1a42801ab490e9b0bfd29a94d21db 2023-05-15T15:16:44+02:00 Insights into an Optimization of Plasmodium vivax Sal-1 In Vitro Culture: The Aotus Primate Model. Kathryn Shaw-Saliba Richard Thomson-Luque Nicanor Obaldía Marlon Nuñez Sahir Dutary Caeul Lim Samantha Barnes Clemens H M Kocken Manoj T Duraisingh John H Adams Erica M Pasini 2016-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004870 https://doaj.org/article/61b1a42801ab490e9b0bfd29a94d21db EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4963040?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004870 https://doaj.org/article/61b1a42801ab490e9b0bfd29a94d21db PLoS Neglected Tropical Diseases, Vol 10, Iss 7, p e0004870 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004870 2022-12-31T16:22:31Z Malaria is one of the most significant tropical diseases, and of the Plasmodium species that cause human malaria, P. vivax is the most geographically widespread. However, P. vivax remains a relatively neglected human parasite since research is typically limited to laboratories with direct access to parasite isolates from endemic field settings or from non-human primate models. This restricted research capacity is in large part due to the lack of a continuous P. vivax in vitro culture system, which has hampered the ability for experimental research needed to gain biological knowledge and develop new therapies. Consequently, efforts to establish a long-term P. vivax culture system are confounded by our poor knowledge of the preferred host cell and essential nutrients needed for in vitro propagation. Reliance on very heterogeneous P. vivax field isolates makes it difficult to benchmark parasite characteristics and further complicates development of a robust and reliable culture method. In an effort to eliminate parasite variability as a complication, we used a well-defined Aotus-adapted P. vivax Sal-1 strain to empirically evaluate different short-term in vitro culture conditions and compare them with previous reported attempts at P. vivax in vitro culture Most importantly, we suggest that reticulocyte enrichment methods affect invasion efficiency and we identify stabilized forms of nutrients that appear beneficial for parasite growth, indicating that P. vivax may be extremely sensitive to waste products. Leuko-depletion methods did not significantly affect parasite development. Formatting changes such as shaking and static cultures did not seem to have a major impact while; in contrast, the starting haematocrit affected both parasite invasion and growth. These results support the continued use of Aotus-adapted Sal-1 for development of P. vivax laboratory methods; however, further experiments are needed to optimize culture conditions to support long-term parasite development. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 7 e0004870 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Kathryn Shaw-Saliba Richard Thomson-Luque Nicanor Obaldía Marlon Nuñez Sahir Dutary Caeul Lim Samantha Barnes Clemens H M Kocken Manoj T Duraisingh John H Adams Erica M Pasini Insights into an Optimization of Plasmodium vivax Sal-1 In Vitro Culture: The Aotus Primate Model. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Malaria is one of the most significant tropical diseases, and of the Plasmodium species that cause human malaria, P. vivax is the most geographically widespread. However, P. vivax remains a relatively neglected human parasite since research is typically limited to laboratories with direct access to parasite isolates from endemic field settings or from non-human primate models. This restricted research capacity is in large part due to the lack of a continuous P. vivax in vitro culture system, which has hampered the ability for experimental research needed to gain biological knowledge and develop new therapies. Consequently, efforts to establish a long-term P. vivax culture system are confounded by our poor knowledge of the preferred host cell and essential nutrients needed for in vitro propagation. Reliance on very heterogeneous P. vivax field isolates makes it difficult to benchmark parasite characteristics and further complicates development of a robust and reliable culture method. In an effort to eliminate parasite variability as a complication, we used a well-defined Aotus-adapted P. vivax Sal-1 strain to empirically evaluate different short-term in vitro culture conditions and compare them with previous reported attempts at P. vivax in vitro culture Most importantly, we suggest that reticulocyte enrichment methods affect invasion efficiency and we identify stabilized forms of nutrients that appear beneficial for parasite growth, indicating that P. vivax may be extremely sensitive to waste products. Leuko-depletion methods did not significantly affect parasite development. Formatting changes such as shaking and static cultures did not seem to have a major impact while; in contrast, the starting haematocrit affected both parasite invasion and growth. These results support the continued use of Aotus-adapted Sal-1 for development of P. vivax laboratory methods; however, further experiments are needed to optimize culture conditions to support long-term parasite development. |
format |
Article in Journal/Newspaper |
author |
Kathryn Shaw-Saliba Richard Thomson-Luque Nicanor Obaldía Marlon Nuñez Sahir Dutary Caeul Lim Samantha Barnes Clemens H M Kocken Manoj T Duraisingh John H Adams Erica M Pasini |
author_facet |
Kathryn Shaw-Saliba Richard Thomson-Luque Nicanor Obaldía Marlon Nuñez Sahir Dutary Caeul Lim Samantha Barnes Clemens H M Kocken Manoj T Duraisingh John H Adams Erica M Pasini |
author_sort |
Kathryn Shaw-Saliba |
title |
Insights into an Optimization of Plasmodium vivax Sal-1 In Vitro Culture: The Aotus Primate Model. |
title_short |
Insights into an Optimization of Plasmodium vivax Sal-1 In Vitro Culture: The Aotus Primate Model. |
title_full |
Insights into an Optimization of Plasmodium vivax Sal-1 In Vitro Culture: The Aotus Primate Model. |
title_fullStr |
Insights into an Optimization of Plasmodium vivax Sal-1 In Vitro Culture: The Aotus Primate Model. |
title_full_unstemmed |
Insights into an Optimization of Plasmodium vivax Sal-1 In Vitro Culture: The Aotus Primate Model. |
title_sort |
insights into an optimization of plasmodium vivax sal-1 in vitro culture: the aotus primate model. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004870 https://doaj.org/article/61b1a42801ab490e9b0bfd29a94d21db |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 7, p e0004870 (2016) |
op_relation |
http://europepmc.org/articles/PMC4963040?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004870 https://doaj.org/article/61b1a42801ab490e9b0bfd29a94d21db |
op_doi |
https://doi.org/10.1371/journal.pntd.0004870 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
10 |
container_issue |
7 |
container_start_page |
e0004870 |
_version_ |
1766347025069113344 |