Resistance to OsHV-1 infection in Crassostrea gigas larvae
The ostreid herpesvirus (OsHV-1) is one of the major diseases that affect the Pacific oyster Crassostrea gigas. Selective breeding programs were recently shown to improve resistance easily to OsHV-1 infections in spat, juvenile and adult oysters. Nevertheless, this resistance has never been investig...
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ftdoajarticles:oai:doaj.org/article:61b027fc9a2a4e389287e6e9256193c4 2023-05-15T15:58:04+02:00 Resistance to OsHV-1 infection in Crassostrea gigas larvae Lionel eDégremont Benjamin eMorga Suzanne eTrancart Jean Francois ePépin 2016-02-01T00:00:00Z https://doi.org/10.3389/fmars.2016.00015 https://doaj.org/article/61b027fc9a2a4e389287e6e9256193c4 EN eng Frontiers Media S.A. http://journal.frontiersin.org/Journal/10.3389/fmars.2016.00015/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2016.00015 https://doaj.org/article/61b027fc9a2a4e389287e6e9256193c4 Frontiers in Marine Science, Vol 3 (2016) Disease Resistance Mortality Selection Genetic Larvae experimental infection Crassostrea gigas Science Q General. Including nature conservation geographical distribution QH1-199.5 article 2016 ftdoajarticles https://doi.org/10.3389/fmars.2016.00015 2022-12-30T22:15:44Z The ostreid herpesvirus (OsHV-1) is one of the major diseases that affect the Pacific oyster Crassostrea gigas. Selective breeding programs were recently shown to improve resistance easily to OsHV-1 infections in spat, juvenile and adult oysters. Nevertheless, this resistance has never been investigated in larvae, whereas this developmental stage has crucial importance for the production of commercial hatcheries, as well as explaining the abundance of spatfall. A first trial tested several viral suspensions at several concentrations using contaminated water with OsHV-1 in four-day-old and ten-day-old larvae that were produced from an unselected broodstock. In follow up on the results, one viral suspension at a final concentration of 10+6 OsHV-1 DNA copies per L was used to assess resistance to OsHV-1 infection in C. gigas larvae that were produced from selected and unselected broodstock. A second trial evaluated OsHV-1 resistance in larvae from both broodstocks in trials 2a, 2b and 2c with 4, 10 and 16-day-old larvae for 7 days, which corresponded to post D larvae, umbo larvae and eyed larvae, respectively. The mortality of unchallenged larvae for both stocks were low (<15%) at day 7 in trials 2a and 2b, whereas it ranged from 48 to 56% in trial 2c. More interestingly, selected larvae had significantly lower mortality than unselected larvae when exposed to OsHV-1 in all of the trials. Thus, the mortality was 11% and 49% for the selected larvae at day 7 post-exposure in trials 2a and 2c, respectively, in comparison with 84% and 97% for the unselected larvae. Although this difference in mortality was observed at day 5 in trial 2b, it was reduced at day 7, to 86% and 98% for the selected and unselected larvae, respectively. For the first time in the literature, the difference in mortality or the delayed onset of mortality between selected and unselected larvae have indicated a genetic resistance to OsHV-1 infection at the larval stage. Such finding should facilitate the selective breeding programs focusing on ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific Frontiers in Marine Science 3 |
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op_collection_id |
ftdoajarticles |
language |
English |
topic |
Disease Resistance Mortality Selection Genetic Larvae experimental infection Crassostrea gigas Science Q General. Including nature conservation geographical distribution QH1-199.5 |
spellingShingle |
Disease Resistance Mortality Selection Genetic Larvae experimental infection Crassostrea gigas Science Q General. Including nature conservation geographical distribution QH1-199.5 Lionel eDégremont Benjamin eMorga Suzanne eTrancart Jean Francois ePépin Resistance to OsHV-1 infection in Crassostrea gigas larvae |
topic_facet |
Disease Resistance Mortality Selection Genetic Larvae experimental infection Crassostrea gigas Science Q General. Including nature conservation geographical distribution QH1-199.5 |
description |
The ostreid herpesvirus (OsHV-1) is one of the major diseases that affect the Pacific oyster Crassostrea gigas. Selective breeding programs were recently shown to improve resistance easily to OsHV-1 infections in spat, juvenile and adult oysters. Nevertheless, this resistance has never been investigated in larvae, whereas this developmental stage has crucial importance for the production of commercial hatcheries, as well as explaining the abundance of spatfall. A first trial tested several viral suspensions at several concentrations using contaminated water with OsHV-1 in four-day-old and ten-day-old larvae that were produced from an unselected broodstock. In follow up on the results, one viral suspension at a final concentration of 10+6 OsHV-1 DNA copies per L was used to assess resistance to OsHV-1 infection in C. gigas larvae that were produced from selected and unselected broodstock. A second trial evaluated OsHV-1 resistance in larvae from both broodstocks in trials 2a, 2b and 2c with 4, 10 and 16-day-old larvae for 7 days, which corresponded to post D larvae, umbo larvae and eyed larvae, respectively. The mortality of unchallenged larvae for both stocks were low (<15%) at day 7 in trials 2a and 2b, whereas it ranged from 48 to 56% in trial 2c. More interestingly, selected larvae had significantly lower mortality than unselected larvae when exposed to OsHV-1 in all of the trials. Thus, the mortality was 11% and 49% for the selected larvae at day 7 post-exposure in trials 2a and 2c, respectively, in comparison with 84% and 97% for the unselected larvae. Although this difference in mortality was observed at day 5 in trial 2b, it was reduced at day 7, to 86% and 98% for the selected and unselected larvae, respectively. For the first time in the literature, the difference in mortality or the delayed onset of mortality between selected and unselected larvae have indicated a genetic resistance to OsHV-1 infection at the larval stage. Such finding should facilitate the selective breeding programs focusing on ... |
format |
Article in Journal/Newspaper |
author |
Lionel eDégremont Benjamin eMorga Suzanne eTrancart Jean Francois ePépin |
author_facet |
Lionel eDégremont Benjamin eMorga Suzanne eTrancart Jean Francois ePépin |
author_sort |
Lionel eDégremont |
title |
Resistance to OsHV-1 infection in Crassostrea gigas larvae |
title_short |
Resistance to OsHV-1 infection in Crassostrea gigas larvae |
title_full |
Resistance to OsHV-1 infection in Crassostrea gigas larvae |
title_fullStr |
Resistance to OsHV-1 infection in Crassostrea gigas larvae |
title_full_unstemmed |
Resistance to OsHV-1 infection in Crassostrea gigas larvae |
title_sort |
resistance to oshv-1 infection in crassostrea gigas larvae |
publisher |
Frontiers Media S.A. |
publishDate |
2016 |
url |
https://doi.org/10.3389/fmars.2016.00015 https://doaj.org/article/61b027fc9a2a4e389287e6e9256193c4 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Frontiers in Marine Science, Vol 3 (2016) |
op_relation |
http://journal.frontiersin.org/Journal/10.3389/fmars.2016.00015/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2016.00015 https://doaj.org/article/61b027fc9a2a4e389287e6e9256193c4 |
op_doi |
https://doi.org/10.3389/fmars.2016.00015 |
container_title |
Frontiers in Marine Science |
container_volume |
3 |
_version_ |
1766393788720218112 |