Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans
We report a rapid method for the flow cytometric quantitation of phagocytosis in heparinized complete peripherial blood (HCPB), using commercially available phycoerythrin-conjugated latex particles of 1µm diameter. The method is faster and shows greater reproducibility than Bjerknes' (1984) sta...
Published in: | Revista da Sociedade Brasileira de Medicina Tropical |
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Sociedade Brasileira de Medicina Tropical (SBMT)
1997
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Online Access: | https://doi.org/10.1590/S0037-86821997000600001 https://doaj.org/article/61ae46c00ad944caae9c633f6235ceec |
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ftdoajarticles:oai:doaj.org/article:61ae46c00ad944caae9c633f6235ceec 2023-05-15T15:06:26+02:00 Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans Jesús M. Egido Juan Viñuelas 1997-12-01T00:00:00Z https://doi.org/10.1590/S0037-86821997000600001 https://doaj.org/article/61ae46c00ad944caae9c633f6235ceec EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821997000600001 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 doi:10.1590/S0037-86821997000600001 0037-8682 1678-9849 https://doaj.org/article/61ae46c00ad944caae9c633f6235ceec Revista da Sociedade Brasileira de Medicina Tropical, Vol 30, Iss 6, Pp 441-446 (1997) Fagocitosis Flujo citométrico Partículas de látex Candida albicans Phagocytosis Flow cytometry Latex particles Arctic medicine. Tropical medicine RC955-962 article 1997 ftdoajarticles https://doi.org/10.1590/S0037-86821997000600001 2022-12-30T23:24:30Z We report a rapid method for the flow cytometric quantitation of phagocytosis in heparinized complete peripherial blood (HCPB), using commercially available phycoerythrin-conjugated latex particles of 1µm diameter. The method is faster and shows greater reproducibility than Bjerknes' (1984) standard technique using propidium iodide-stained Candida albicans, conventionally applied to the leukocytic layer of peripherial blood but here modified for HCPB. We also report a modification of Bjerknes' Intracellular Killing Test to allow its application to HCPB. Se da cuenta de un método rápido para la cuantización del flujo citométrico de la fagocitosis en sangre periférica completamente heparinizada (HCPB), mediante la utilización de partículas de látex phycoerythrin-conjugadas de 1µm de diámetro disponibles comercialmente. El método es más rápido y presenta mayor reproducibilidad que la técnica estandar de Bjerknes' (1984) utilizando propidium iodide-teñida Candida albicans, aplicada convencionalmente a la capa leucocitica de sangre periférica pero modificada por HCPB. Tambien damos cuenta de una modificación de Bjerknes' Intracellular Killing Test para permitir su aplicación a HCPB. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 30 6 441 446 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Fagocitosis Flujo citométrico Partículas de látex Candida albicans Phagocytosis Flow cytometry Latex particles Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Fagocitosis Flujo citométrico Partículas de látex Candida albicans Phagocytosis Flow cytometry Latex particles Arctic medicine. Tropical medicine RC955-962 Jesús M. Egido Juan Viñuelas Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans |
topic_facet |
Fagocitosis Flujo citométrico Partículas de látex Candida albicans Phagocytosis Flow cytometry Latex particles Arctic medicine. Tropical medicine RC955-962 |
description |
We report a rapid method for the flow cytometric quantitation of phagocytosis in heparinized complete peripherial blood (HCPB), using commercially available phycoerythrin-conjugated latex particles of 1µm diameter. The method is faster and shows greater reproducibility than Bjerknes' (1984) standard technique using propidium iodide-stained Candida albicans, conventionally applied to the leukocytic layer of peripherial blood but here modified for HCPB. We also report a modification of Bjerknes' Intracellular Killing Test to allow its application to HCPB. Se da cuenta de un método rápido para la cuantización del flujo citométrico de la fagocitosis en sangre periférica completamente heparinizada (HCPB), mediante la utilización de partículas de látex phycoerythrin-conjugadas de 1µm de diámetro disponibles comercialmente. El método es más rápido y presenta mayor reproducibilidad que la técnica estandar de Bjerknes' (1984) utilizando propidium iodide-teñida Candida albicans, aplicada convencionalmente a la capa leucocitica de sangre periférica pero modificada por HCPB. Tambien damos cuenta de una modificación de Bjerknes' Intracellular Killing Test para permitir su aplicación a HCPB. |
format |
Article in Journal/Newspaper |
author |
Jesús M. Egido Juan Viñuelas |
author_facet |
Jesús M. Egido Juan Viñuelas |
author_sort |
Jesús M. Egido |
title |
Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans |
title_short |
Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans |
title_full |
Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans |
title_fullStr |
Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans |
title_full_unstemmed |
Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans |
title_sort |
flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and candida albicans |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
1997 |
url |
https://doi.org/10.1590/S0037-86821997000600001 https://doaj.org/article/61ae46c00ad944caae9c633f6235ceec |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 30, Iss 6, Pp 441-446 (1997) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821997000600001 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 doi:10.1590/S0037-86821997000600001 0037-8682 1678-9849 https://doaj.org/article/61ae46c00ad944caae9c633f6235ceec |
op_doi |
https://doi.org/10.1590/S0037-86821997000600001 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
container_volume |
30 |
container_issue |
6 |
container_start_page |
441 |
op_container_end_page |
446 |
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