Study of infection by Rickettsiae of the spotted fever group in humans and ticks in an urban park located in the City of Londrina, State of Paraná, Brazil Estudo da infecção por Rickettsias do grupo da febre maculosa em humanos e carrapatos de um parque urbano na Cidade de Londrina, Estado do Paraná
INTRODUCTION: Spotted fevers are emerging zoonoses caused by Rickettsia species in the spotted fever group (SFG). Rickettsia rickettsii is the main etiologic agent of Brazilian spotted fever (BSF) and it is transmitted by Amblyomma spp. ticks. METHODS: The study aimed to investigate SFG rickettsiae...
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Sociedade Brasileira de Medicina Tropical (SBMT)
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ftdoajarticles:oai:doaj.org/article:61a191e2d6324369888bd5e1202a67e4 2023-05-15T15:18:48+02:00 Study of infection by Rickettsiae of the spotted fever group in humans and ticks in an urban park located in the City of Londrina, State of Paraná, Brazil Estudo da infecção por Rickettsias do grupo da febre maculosa em humanos e carrapatos de um parque urbano na Cidade de Londrina, Estado do Paraná Roberta Santos Toledo Katia Tamekuni Mauro de Freitas Silva Filho Valeska Bender Haydu Richard Campos Pacheco Marcelo Bahia Labruna John Stephen Dumler Odilon Vidotto 2011-06-01T00:00:00Z https://doaj.org/article/61a191e2d6324369888bd5e1202a67e4 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300010 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 0037-8682 1678-9849 https://doaj.org/article/61a191e2d6324369888bd5e1202a67e4 Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 3, Pp 313-317 (2011) Amblyomma dubitatum Amblyomma cajennense Rickettsia Epidemiologia PCR Epidemiology Arctic medicine. Tropical medicine RC955-962 article 2011 ftdoajarticles 2022-12-30T21:26:57Z INTRODUCTION: Spotted fevers are emerging zoonoses caused by Rickettsia species in the spotted fever group (SFG). Rickettsia rickettsii is the main etiologic agent of Brazilian spotted fever (BSF) and it is transmitted by Amblyomma spp. ticks. METHODS: The study aimed to investigate SFG rickettsiae in the Arthur Thomas Municipal Park in Londrina, PR, by collecting free-living ticks and ticks from capybaras and blood samples from personnel working in these areas. Samples from A. dubitatum and A. cajennense were submitted for PCR in pools to analyze the Rickettsia spp. gltA (citrate synthase gene). RESULTS: All the pools analyzed were negative. Human sera were tested by indirect immunofluorescence assay with R. rickettsii and R. parkeri as antigens. Among the 34 sera analyzed, seven (20.6%) were reactive for R. rickettsii: four of these had endpoint titers equal to 64, 2 titers were 128 and 1 titer was 256. None of the samples were reactive for R. parkeri. An epidemiological questionnaire was applied to the park staff, but no statistically significant associations were identified. CONCLUSIONS: The serological studies suggest the presence of Rickettsiae related to SFG that could be infecting the human population studied; however, analysis of the ticks collected was unable to determine which species may be involved in transmission to humans. INTRODUÇÃO: A febre maculosa é uma zoonose emergente causada por espécies de Rickettsia do grupo febre maculosa (GFM). Rickettsia rickettsii é o principal agente etiológico da febre maculosa brasileira (FMB) e é transmitida por Amblyomma spp. MÉTODOS: Com o objetivo de obter informações sobre GFM Rickettsiae no Parque Municipal Arthur Thomas em Londrina, PR, carrapatos de vida livre e de capivaras foram coletados, assim como amostras de sangue das pessoas que trabalham no parque. A. dubitatum e A. cajennense foram submetidos à PCR em pools para analises de Rickettsia spp. gltA (citrate synthase gene). RESULTADOS: Todos os pools de carrapatos analizados foram negativos. Soros de ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Amblyomma dubitatum Amblyomma cajennense Rickettsia Epidemiologia PCR Epidemiology Arctic medicine. Tropical medicine RC955-962 |
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Amblyomma dubitatum Amblyomma cajennense Rickettsia Epidemiologia PCR Epidemiology Arctic medicine. Tropical medicine RC955-962 Roberta Santos Toledo Katia Tamekuni Mauro de Freitas Silva Filho Valeska Bender Haydu Richard Campos Pacheco Marcelo Bahia Labruna John Stephen Dumler Odilon Vidotto Study of infection by Rickettsiae of the spotted fever group in humans and ticks in an urban park located in the City of Londrina, State of Paraná, Brazil Estudo da infecção por Rickettsias do grupo da febre maculosa em humanos e carrapatos de um parque urbano na Cidade de Londrina, Estado do Paraná |
topic_facet |
Amblyomma dubitatum Amblyomma cajennense Rickettsia Epidemiologia PCR Epidemiology Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: Spotted fevers are emerging zoonoses caused by Rickettsia species in the spotted fever group (SFG). Rickettsia rickettsii is the main etiologic agent of Brazilian spotted fever (BSF) and it is transmitted by Amblyomma spp. ticks. METHODS: The study aimed to investigate SFG rickettsiae in the Arthur Thomas Municipal Park in Londrina, PR, by collecting free-living ticks and ticks from capybaras and blood samples from personnel working in these areas. Samples from A. dubitatum and A. cajennense were submitted for PCR in pools to analyze the Rickettsia spp. gltA (citrate synthase gene). RESULTS: All the pools analyzed were negative. Human sera were tested by indirect immunofluorescence assay with R. rickettsii and R. parkeri as antigens. Among the 34 sera analyzed, seven (20.6%) were reactive for R. rickettsii: four of these had endpoint titers equal to 64, 2 titers were 128 and 1 titer was 256. None of the samples were reactive for R. parkeri. An epidemiological questionnaire was applied to the park staff, but no statistically significant associations were identified. CONCLUSIONS: The serological studies suggest the presence of Rickettsiae related to SFG that could be infecting the human population studied; however, analysis of the ticks collected was unable to determine which species may be involved in transmission to humans. INTRODUÇÃO: A febre maculosa é uma zoonose emergente causada por espécies de Rickettsia do grupo febre maculosa (GFM). Rickettsia rickettsii é o principal agente etiológico da febre maculosa brasileira (FMB) e é transmitida por Amblyomma spp. MÉTODOS: Com o objetivo de obter informações sobre GFM Rickettsiae no Parque Municipal Arthur Thomas em Londrina, PR, carrapatos de vida livre e de capivaras foram coletados, assim como amostras de sangue das pessoas que trabalham no parque. A. dubitatum e A. cajennense foram submetidos à PCR em pools para analises de Rickettsia spp. gltA (citrate synthase gene). RESULTADOS: Todos os pools de carrapatos analizados foram negativos. Soros de ... |
format |
Article in Journal/Newspaper |
author |
Roberta Santos Toledo Katia Tamekuni Mauro de Freitas Silva Filho Valeska Bender Haydu Richard Campos Pacheco Marcelo Bahia Labruna John Stephen Dumler Odilon Vidotto |
author_facet |
Roberta Santos Toledo Katia Tamekuni Mauro de Freitas Silva Filho Valeska Bender Haydu Richard Campos Pacheco Marcelo Bahia Labruna John Stephen Dumler Odilon Vidotto |
author_sort |
Roberta Santos Toledo |
title |
Study of infection by Rickettsiae of the spotted fever group in humans and ticks in an urban park located in the City of Londrina, State of Paraná, Brazil Estudo da infecção por Rickettsias do grupo da febre maculosa em humanos e carrapatos de um parque urbano na Cidade de Londrina, Estado do Paraná |
title_short |
Study of infection by Rickettsiae of the spotted fever group in humans and ticks in an urban park located in the City of Londrina, State of Paraná, Brazil Estudo da infecção por Rickettsias do grupo da febre maculosa em humanos e carrapatos de um parque urbano na Cidade de Londrina, Estado do Paraná |
title_full |
Study of infection by Rickettsiae of the spotted fever group in humans and ticks in an urban park located in the City of Londrina, State of Paraná, Brazil Estudo da infecção por Rickettsias do grupo da febre maculosa em humanos e carrapatos de um parque urbano na Cidade de Londrina, Estado do Paraná |
title_fullStr |
Study of infection by Rickettsiae of the spotted fever group in humans and ticks in an urban park located in the City of Londrina, State of Paraná, Brazil Estudo da infecção por Rickettsias do grupo da febre maculosa em humanos e carrapatos de um parque urbano na Cidade de Londrina, Estado do Paraná |
title_full_unstemmed |
Study of infection by Rickettsiae of the spotted fever group in humans and ticks in an urban park located in the City of Londrina, State of Paraná, Brazil Estudo da infecção por Rickettsias do grupo da febre maculosa em humanos e carrapatos de um parque urbano na Cidade de Londrina, Estado do Paraná |
title_sort |
study of infection by rickettsiae of the spotted fever group in humans and ticks in an urban park located in the city of londrina, state of paraná, brazil estudo da infecção por rickettsias do grupo da febre maculosa em humanos e carrapatos de um parque urbano na cidade de londrina, estado do paraná |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2011 |
url |
https://doaj.org/article/61a191e2d6324369888bd5e1202a67e4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 3, Pp 313-317 (2011) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300010 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 0037-8682 1678-9849 https://doaj.org/article/61a191e2d6324369888bd5e1202a67e4 |
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1766348974643478528 |