Are 45 years of reproductive isolation enough to prevent the amplification of mitochondrial genes in the Pacific oyster?
ABSTRACT | The Pacific oyster culture in Mexico began 45 years ago, first with spat imported from the USA and now with spat produced in several local hatcheries. Oyster farmers do not know the parameters that define the quality of the spat they buy, among them the level of genetic variability availa...
Published in: | AquaTechnica: Revista Iberoamericana de Acuicultura. |
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Format: | Article in Journal/Newspaper |
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Universidad Técnica de Manabí
2019
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Online Access: | https://doi.org/10.33936/at.v1i1.2147 https://doaj.org/article/616bf1e4db2e4a4ba1a129d1ecf56145 |
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ftdoajarticles:oai:doaj.org/article:616bf1e4db2e4a4ba1a129d1ecf56145 2023-05-15T15:59:10+02:00 Are 45 years of reproductive isolation enough to prevent the amplification of mitochondrial genes in the Pacific oyster? Felipe Reynaga Franco José Manuel Grijalva Chon Jorge Eduardo Chávez Villalba Reina Castro Longoria José Alfredo Arreola Lizárraga Ramón Héctor Barraza Guardado 2019-12-01T00:00:00Z https://doi.org/10.33936/at.v1i1.2147 https://doaj.org/article/616bf1e4db2e4a4ba1a129d1ecf56145 EN ES PT eng spa por Universidad Técnica de Manabí https://revistas.utm.edu.ec/index.php/aquatechnica/article/view/2147 https://doaj.org/toc/2737-6095 https://doi.org/10.33936/at.v1i1.2147 2737-6095 https://doaj.org/article/616bf1e4db2e4a4ba1a129d1ecf56145 AquaTechnica, Vol 1, Iss 1, Pp 22-27 (2019) crassostrea gigas aquaculture oyster culture mitochondrial dna population genetics acuacultura ostricultura adn mitochondrial genética de poblaciones Geography. Anthropology. Recreation G Economic biology QH705-705.5 Ecology QH540-549.5 article 2019 ftdoajarticles https://doi.org/10.33936/at.v1i1.2147 2022-12-31T03:20:37Z ABSTRACT | The Pacific oyster culture in Mexico began 45 years ago, first with spat imported from the USA and now with spat produced in several local hatcheries. Oyster farmers do not know the parameters that define the quality of the spat they buy, among them the level of genetic variability available in the lots offered. In order to evaluate and compare the genetic variability in spat produced by four Mexican hatcheries, an attempt was made to amplify and sequence the non-coding region and the ND5 gene of the oyster mitochondrial DNA with oligos reported in the scientific literature. The amplification of the non-coding region was not possible due to the bad design of the oligos. Despite the integrity of the extracted oyster DNA, the ND5 gene was not able to be amplified possibly due to the modification of the oligo recognition site in the gene. The generational separation of the oyster cultivated in Mexico from its original source population makes it necessary to obtain new mitochondrial sequences in order to design new oligos suitable for the populations established in Mexico. RESUMEN | El cultivo del ostión del Pacífico en México inició hace 45 años, primero con semilla importada de los EUA y ahora con semilla producida en laboratorios locales. Los ostricultores no conocen los parámetros que definen la calidad de la semilla que ellos compran, como el nivel de la variabilidad genética disponible en los lotes ofertados. Con el fin de evaluar y comparar la variabilidad genética de la semilla producida en cuatro laboratorios mexicanos, se hizo un intento de amplificar y secuenciar la región no codificante y el gen ND5 mitocondriales del ostión con oligos reportados en la literatura científica. La amplificación de la región no codificante no fue posible debido al mal diseño de los oligos. A pesar de la integridad del ADN extraído de los ostiones, el gen ND5 no pudo ser amplificado debido posiblemente a la modificación del sitio de reconocimiento del oligo en ese gen. La separación generacional del ostión ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific AquaTechnica: Revista Iberoamericana de Acuicultura. 1 1 22 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Spanish Portuguese |
topic |
crassostrea gigas aquaculture oyster culture mitochondrial dna population genetics acuacultura ostricultura adn mitochondrial genética de poblaciones Geography. Anthropology. Recreation G Economic biology QH705-705.5 Ecology QH540-549.5 |
spellingShingle |
crassostrea gigas aquaculture oyster culture mitochondrial dna population genetics acuacultura ostricultura adn mitochondrial genética de poblaciones Geography. Anthropology. Recreation G Economic biology QH705-705.5 Ecology QH540-549.5 Felipe Reynaga Franco José Manuel Grijalva Chon Jorge Eduardo Chávez Villalba Reina Castro Longoria José Alfredo Arreola Lizárraga Ramón Héctor Barraza Guardado Are 45 years of reproductive isolation enough to prevent the amplification of mitochondrial genes in the Pacific oyster? |
topic_facet |
crassostrea gigas aquaculture oyster culture mitochondrial dna population genetics acuacultura ostricultura adn mitochondrial genética de poblaciones Geography. Anthropology. Recreation G Economic biology QH705-705.5 Ecology QH540-549.5 |
description |
ABSTRACT | The Pacific oyster culture in Mexico began 45 years ago, first with spat imported from the USA and now with spat produced in several local hatcheries. Oyster farmers do not know the parameters that define the quality of the spat they buy, among them the level of genetic variability available in the lots offered. In order to evaluate and compare the genetic variability in spat produced by four Mexican hatcheries, an attempt was made to amplify and sequence the non-coding region and the ND5 gene of the oyster mitochondrial DNA with oligos reported in the scientific literature. The amplification of the non-coding region was not possible due to the bad design of the oligos. Despite the integrity of the extracted oyster DNA, the ND5 gene was not able to be amplified possibly due to the modification of the oligo recognition site in the gene. The generational separation of the oyster cultivated in Mexico from its original source population makes it necessary to obtain new mitochondrial sequences in order to design new oligos suitable for the populations established in Mexico. RESUMEN | El cultivo del ostión del Pacífico en México inició hace 45 años, primero con semilla importada de los EUA y ahora con semilla producida en laboratorios locales. Los ostricultores no conocen los parámetros que definen la calidad de la semilla que ellos compran, como el nivel de la variabilidad genética disponible en los lotes ofertados. Con el fin de evaluar y comparar la variabilidad genética de la semilla producida en cuatro laboratorios mexicanos, se hizo un intento de amplificar y secuenciar la región no codificante y el gen ND5 mitocondriales del ostión con oligos reportados en la literatura científica. La amplificación de la región no codificante no fue posible debido al mal diseño de los oligos. A pesar de la integridad del ADN extraído de los ostiones, el gen ND5 no pudo ser amplificado debido posiblemente a la modificación del sitio de reconocimiento del oligo en ese gen. La separación generacional del ostión ... |
format |
Article in Journal/Newspaper |
author |
Felipe Reynaga Franco José Manuel Grijalva Chon Jorge Eduardo Chávez Villalba Reina Castro Longoria José Alfredo Arreola Lizárraga Ramón Héctor Barraza Guardado |
author_facet |
Felipe Reynaga Franco José Manuel Grijalva Chon Jorge Eduardo Chávez Villalba Reina Castro Longoria José Alfredo Arreola Lizárraga Ramón Héctor Barraza Guardado |
author_sort |
Felipe Reynaga Franco |
title |
Are 45 years of reproductive isolation enough to prevent the amplification of mitochondrial genes in the Pacific oyster? |
title_short |
Are 45 years of reproductive isolation enough to prevent the amplification of mitochondrial genes in the Pacific oyster? |
title_full |
Are 45 years of reproductive isolation enough to prevent the amplification of mitochondrial genes in the Pacific oyster? |
title_fullStr |
Are 45 years of reproductive isolation enough to prevent the amplification of mitochondrial genes in the Pacific oyster? |
title_full_unstemmed |
Are 45 years of reproductive isolation enough to prevent the amplification of mitochondrial genes in the Pacific oyster? |
title_sort |
are 45 years of reproductive isolation enough to prevent the amplification of mitochondrial genes in the pacific oyster? |
publisher |
Universidad Técnica de Manabí |
publishDate |
2019 |
url |
https://doi.org/10.33936/at.v1i1.2147 https://doaj.org/article/616bf1e4db2e4a4ba1a129d1ecf56145 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
AquaTechnica, Vol 1, Iss 1, Pp 22-27 (2019) |
op_relation |
https://revistas.utm.edu.ec/index.php/aquatechnica/article/view/2147 https://doaj.org/toc/2737-6095 https://doi.org/10.33936/at.v1i1.2147 2737-6095 https://doaj.org/article/616bf1e4db2e4a4ba1a129d1ecf56145 |
op_doi |
https://doi.org/10.33936/at.v1i1.2147 |
container_title |
AquaTechnica: Revista Iberoamericana de Acuicultura. |
container_volume |
1 |
container_issue |
1 |
container_start_page |
22 |
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1766394967748509696 |