Intestinal spirochetosis: first cases reported in Brazil and the use of immunohistochemistry as an aid in histopathological diagnosis Espiroquetose intestinal: primeiros casos no Brasil e o uso de técnicas imunohistoquímicas como ajuda no diagnóstico histopatológico
Colonization of the colon and rectum by intestinal spirochetes is detected for the first time in Brazil in 4 of 282 (1.41%) patients who had undergone sigmoidoscopy and/or colonoscopy with a histopathological diagnosis of chronic non specific-colitis. This frequency is probably understimated, since...
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Universidade de São Paulo (USP)
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ftdoajarticles:oai:doaj.org/article:614d2e73a9214439b76fd99d34f2d7ba 2024-09-09T19:28:26+00:00 Intestinal spirochetosis: first cases reported in Brazil and the use of immunohistochemistry as an aid in histopathological diagnosis Espiroquetose intestinal: primeiros casos no Brasil e o uso de técnicas imunohistoquímicas como ajuda no diagnóstico histopatológico T. de Brito Marina P. Sandoval Ana G. Silva R. C. Saad W. Colaiacovo 1996-02-01T00:00:00Z https://doi.org/10.1590/S0036-46651996000100009 https://doaj.org/article/614d2e73a9214439b76fd99d34f2d7ba EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651996000100009 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651996000100009 0036-4665 1678-9946 https://doaj.org/article/614d2e73a9214439b76fd99d34f2d7ba Revista do Instituto de Medicina Tropical de São Paulo, Vol 38, Iss 1, Pp 45-53 (1996) Intestinal Spirochetosis Brachyspira aalborgi Immunohistochemistry Anti-leptospiral and anti-treponemal sera Antigen detection Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1996 ftdoajarticles https://doi.org/10.1590/S0036-46651996000100009 2024-08-05T17:49:30Z Colonization of the colon and rectum by intestinal spirochetes is detected for the first time in Brazil in 4 of 282 (1.41%) patients who had undergone sigmoidoscopy and/or colonoscopy with a histopathological diagnosis of chronic non specific-colitis. This frequency is probably understimated, since surgically obtained specimens were not considered in the present study. Histopathological diagnosis was performed using routine stains like hematoxylin-eosin which showed the typical, of 3-µm thick hematoxyphilic fringe on the brush border of the surface epithelium, and by silver stains like the Warthin-Starry stain. Immunohistochemical procedures using two, polyclonal, primary antibodies, one against Treponema pallidum and the other against Leptospira interrogans serovar copenhageni serogroup Icterohaemorrhagiae cross-reacted with spirochetal antigen/s producing a marked contrast of the fringe over the colonic epithelium, preserving the spiral-shaped morphology of the parasite. In one case with marked diarrhea, immunohistochemistry detected spirochetal antigen/s within a cell in an intestinal crypt, thus demonstrating that the infection can be more widely disseminated than suspected using routine stains. Immunohistochemical procedures, thus, greatly facilitate the histological diagnosis of intestinal spirochetosis and may contribute to a better understanding of the pathogenesis of the disease. Transmission and scanning electron microscopy performed in one case showed that the spirochete closely resembled the species designated as Brachyspira aalborgi. A colonização do colo e reto por espiroquetas intestinais foi detectada pela primeira vez no Brasil em 4 de 282 (1,41%) pacientes submetidos a sigmoidoscopia e/ou colonoscopia com o diagnóstico histopatológico de colite crônica inespecífica. A frequência é provavelmente menor que a real, urna vez que espécimens cirúrgicos não foram considerados no presente estudo. O diagnóstico histopatológico foi feito através de colorações de rotina, tais como a hematoxilina-eosina, ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Urna ENVELOPE(16.767,16.767,-72.233,-72.233) Revista do Instituto de Medicina Tropical de São Paulo 38 1 45 53 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Intestinal Spirochetosis Brachyspira aalborgi Immunohistochemistry Anti-leptospiral and anti-treponemal sera Antigen detection Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Intestinal Spirochetosis Brachyspira aalborgi Immunohistochemistry Anti-leptospiral and anti-treponemal sera Antigen detection Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 T. de Brito Marina P. Sandoval Ana G. Silva R. C. Saad W. Colaiacovo Intestinal spirochetosis: first cases reported in Brazil and the use of immunohistochemistry as an aid in histopathological diagnosis Espiroquetose intestinal: primeiros casos no Brasil e o uso de técnicas imunohistoquímicas como ajuda no diagnóstico histopatológico |
topic_facet |
Intestinal Spirochetosis Brachyspira aalborgi Immunohistochemistry Anti-leptospiral and anti-treponemal sera Antigen detection Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Colonization of the colon and rectum by intestinal spirochetes is detected for the first time in Brazil in 4 of 282 (1.41%) patients who had undergone sigmoidoscopy and/or colonoscopy with a histopathological diagnosis of chronic non specific-colitis. This frequency is probably understimated, since surgically obtained specimens were not considered in the present study. Histopathological diagnosis was performed using routine stains like hematoxylin-eosin which showed the typical, of 3-µm thick hematoxyphilic fringe on the brush border of the surface epithelium, and by silver stains like the Warthin-Starry stain. Immunohistochemical procedures using two, polyclonal, primary antibodies, one against Treponema pallidum and the other against Leptospira interrogans serovar copenhageni serogroup Icterohaemorrhagiae cross-reacted with spirochetal antigen/s producing a marked contrast of the fringe over the colonic epithelium, preserving the spiral-shaped morphology of the parasite. In one case with marked diarrhea, immunohistochemistry detected spirochetal antigen/s within a cell in an intestinal crypt, thus demonstrating that the infection can be more widely disseminated than suspected using routine stains. Immunohistochemical procedures, thus, greatly facilitate the histological diagnosis of intestinal spirochetosis and may contribute to a better understanding of the pathogenesis of the disease. Transmission and scanning electron microscopy performed in one case showed that the spirochete closely resembled the species designated as Brachyspira aalborgi. A colonização do colo e reto por espiroquetas intestinais foi detectada pela primeira vez no Brasil em 4 de 282 (1,41%) pacientes submetidos a sigmoidoscopia e/ou colonoscopia com o diagnóstico histopatológico de colite crônica inespecífica. A frequência é provavelmente menor que a real, urna vez que espécimens cirúrgicos não foram considerados no presente estudo. O diagnóstico histopatológico foi feito através de colorações de rotina, tais como a hematoxilina-eosina, ... |
format |
Article in Journal/Newspaper |
author |
T. de Brito Marina P. Sandoval Ana G. Silva R. C. Saad W. Colaiacovo |
author_facet |
T. de Brito Marina P. Sandoval Ana G. Silva R. C. Saad W. Colaiacovo |
author_sort |
T. de Brito |
title |
Intestinal spirochetosis: first cases reported in Brazil and the use of immunohistochemistry as an aid in histopathological diagnosis Espiroquetose intestinal: primeiros casos no Brasil e o uso de técnicas imunohistoquímicas como ajuda no diagnóstico histopatológico |
title_short |
Intestinal spirochetosis: first cases reported in Brazil and the use of immunohistochemistry as an aid in histopathological diagnosis Espiroquetose intestinal: primeiros casos no Brasil e o uso de técnicas imunohistoquímicas como ajuda no diagnóstico histopatológico |
title_full |
Intestinal spirochetosis: first cases reported in Brazil and the use of immunohistochemistry as an aid in histopathological diagnosis Espiroquetose intestinal: primeiros casos no Brasil e o uso de técnicas imunohistoquímicas como ajuda no diagnóstico histopatológico |
title_fullStr |
Intestinal spirochetosis: first cases reported in Brazil and the use of immunohistochemistry as an aid in histopathological diagnosis Espiroquetose intestinal: primeiros casos no Brasil e o uso de técnicas imunohistoquímicas como ajuda no diagnóstico histopatológico |
title_full_unstemmed |
Intestinal spirochetosis: first cases reported in Brazil and the use of immunohistochemistry as an aid in histopathological diagnosis Espiroquetose intestinal: primeiros casos no Brasil e o uso de técnicas imunohistoquímicas como ajuda no diagnóstico histopatológico |
title_sort |
intestinal spirochetosis: first cases reported in brazil and the use of immunohistochemistry as an aid in histopathological diagnosis espiroquetose intestinal: primeiros casos no brasil e o uso de técnicas imunohistoquímicas como ajuda no diagnóstico histopatológico |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1996 |
url |
https://doi.org/10.1590/S0036-46651996000100009 https://doaj.org/article/614d2e73a9214439b76fd99d34f2d7ba |
long_lat |
ENVELOPE(16.767,16.767,-72.233,-72.233) |
geographic |
Arctic Urna |
geographic_facet |
Arctic Urna |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 38, Iss 1, Pp 45-53 (1996) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651996000100009 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651996000100009 0036-4665 1678-9946 https://doaj.org/article/614d2e73a9214439b76fd99d34f2d7ba |
op_doi |
https://doi.org/10.1590/S0036-46651996000100009 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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38 |
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