Leishmania donovani infection enhances lateral mobility of macrophage membrane protein which is reversed by liposomal cholesterol.
The protozoan parasite Leishmania donovani (LD) reduces cellular cholesterol of the host possibly for its own benefit. Cholesterol is mostly present in the specialized compartment of the plasma membrane. The relation between mobility of membrane proteins and cholesterol depletion from membrane conti...
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ftdoajarticles:oai:doaj.org/article:60d2544b1ca94fd6ad38fb778d8bb8e0 2023-05-15T15:11:50+02:00 Leishmania donovani infection enhances lateral mobility of macrophage membrane protein which is reversed by liposomal cholesterol. Moumita Ghosh Koushik Roy Dipanwita Das Mukherjee Gopal Chakrabarti Kingshuk Roy Choudhury Syamal Roy 2014-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003367 https://doaj.org/article/60d2544b1ca94fd6ad38fb778d8bb8e0 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4256160?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003367 https://doaj.org/article/60d2544b1ca94fd6ad38fb778d8bb8e0 PLoS Neglected Tropical Diseases, Vol 8, Iss 12, p e3367 (2014) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2014 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003367 2022-12-31T12:01:59Z The protozoan parasite Leishmania donovani (LD) reduces cellular cholesterol of the host possibly for its own benefit. Cholesterol is mostly present in the specialized compartment of the plasma membrane. The relation between mobility of membrane proteins and cholesterol depletion from membrane continues to be an important issue. The notion that leishmania infection alters the mobility of membrane proteins stems from our previous study where we showed that the distance between subunits of IFNγ receptor (R1 and R2) on the cell surface of LD infected cell is increased, but is restored to normal by liposomal cholesterol treatment.We determined the lateral mobility of a membrane protein in normal, LD infected and liposome treated LD infected cells using GFP-tagged PLCδ1 as a probe. The mobility of PLCδ1 was computationally analyzed from the time lapse experiment using boundary distance plot and radial profile movement. Our results showed that the lateral mobility of the membrane protein, which is increased in infection, is restored to normal upon liposomal cholesterol treatment. The results of FRAP experiment lent further credence to the above notion. The membrane proteins are intimately linked with cellular actin and alteration of cellular actin may influence lateral mobility. We found that F-actin is decreased in infection but is restored to normal upon liposomal cholesterol treatment as evident from phalloidin staining and also from biochemical analysis by immunoblotting.To our knowledge this is the first direct demonstration that LD parasites during their intracellular life cycle increases lateral mobility of membrane proteins and decreases F-actin level in infected macrophages. Such defects may contribute to ineffective intracellular signaling and other cellular functions. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Lent ENVELOPE(-66.783,-66.783,-66.867,-66.867) PLoS Neglected Tropical Diseases 8 12 e3367 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Moumita Ghosh Koushik Roy Dipanwita Das Mukherjee Gopal Chakrabarti Kingshuk Roy Choudhury Syamal Roy Leishmania donovani infection enhances lateral mobility of macrophage membrane protein which is reversed by liposomal cholesterol. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The protozoan parasite Leishmania donovani (LD) reduces cellular cholesterol of the host possibly for its own benefit. Cholesterol is mostly present in the specialized compartment of the plasma membrane. The relation between mobility of membrane proteins and cholesterol depletion from membrane continues to be an important issue. The notion that leishmania infection alters the mobility of membrane proteins stems from our previous study where we showed that the distance between subunits of IFNγ receptor (R1 and R2) on the cell surface of LD infected cell is increased, but is restored to normal by liposomal cholesterol treatment.We determined the lateral mobility of a membrane protein in normal, LD infected and liposome treated LD infected cells using GFP-tagged PLCδ1 as a probe. The mobility of PLCδ1 was computationally analyzed from the time lapse experiment using boundary distance plot and radial profile movement. Our results showed that the lateral mobility of the membrane protein, which is increased in infection, is restored to normal upon liposomal cholesterol treatment. The results of FRAP experiment lent further credence to the above notion. The membrane proteins are intimately linked with cellular actin and alteration of cellular actin may influence lateral mobility. We found that F-actin is decreased in infection but is restored to normal upon liposomal cholesterol treatment as evident from phalloidin staining and also from biochemical analysis by immunoblotting.To our knowledge this is the first direct demonstration that LD parasites during their intracellular life cycle increases lateral mobility of membrane proteins and decreases F-actin level in infected macrophages. Such defects may contribute to ineffective intracellular signaling and other cellular functions. |
format |
Article in Journal/Newspaper |
author |
Moumita Ghosh Koushik Roy Dipanwita Das Mukherjee Gopal Chakrabarti Kingshuk Roy Choudhury Syamal Roy |
author_facet |
Moumita Ghosh Koushik Roy Dipanwita Das Mukherjee Gopal Chakrabarti Kingshuk Roy Choudhury Syamal Roy |
author_sort |
Moumita Ghosh |
title |
Leishmania donovani infection enhances lateral mobility of macrophage membrane protein which is reversed by liposomal cholesterol. |
title_short |
Leishmania donovani infection enhances lateral mobility of macrophage membrane protein which is reversed by liposomal cholesterol. |
title_full |
Leishmania donovani infection enhances lateral mobility of macrophage membrane protein which is reversed by liposomal cholesterol. |
title_fullStr |
Leishmania donovani infection enhances lateral mobility of macrophage membrane protein which is reversed by liposomal cholesterol. |
title_full_unstemmed |
Leishmania donovani infection enhances lateral mobility of macrophage membrane protein which is reversed by liposomal cholesterol. |
title_sort |
leishmania donovani infection enhances lateral mobility of macrophage membrane protein which is reversed by liposomal cholesterol. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pntd.0003367 https://doaj.org/article/60d2544b1ca94fd6ad38fb778d8bb8e0 |
long_lat |
ENVELOPE(-66.783,-66.783,-66.867,-66.867) |
geographic |
Arctic Lent |
geographic_facet |
Arctic Lent |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 8, Iss 12, p e3367 (2014) |
op_relation |
http://europepmc.org/articles/PMC4256160?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003367 https://doaj.org/article/60d2544b1ca94fd6ad38fb778d8bb8e0 |
op_doi |
https://doi.org/10.1371/journal.pntd.0003367 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
8 |
container_issue |
12 |
container_start_page |
e3367 |
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1766342624137969664 |