Protective effect of the probiotic Lactobacillus acidophilus ATCC 4356 in BALB/c mice infected with Toxocara canis
ABSTRACT Human toxocariasis consists of chronic tissue parasitosis that is difficult to treat and control. This study aimed to evaluate the action of the probiotic Lactobacillus acidophilus ATCC 4356 on larvae of Toxocara canis and the effect of IFN-γ cytokine on parasite-host in vivo (1.109 CFU) an...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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Universidade de São Paulo (USP)
2021
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Online Access: | https://doi.org/10.1590/s1678-9946202163009 https://doaj.org/article/60d0e89aaca343729d6866b8d5da87f3 |
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ftdoajarticles:oai:doaj.org/article:60d0e89aaca343729d6866b8d5da87f3 2024-09-09T19:27:29+00:00 Protective effect of the probiotic Lactobacillus acidophilus ATCC 4356 in BALB/c mice infected with Toxocara canis Priscila Silva Cadore Débora Liliane Walcher Nicholas Frota Gonçalves Correia de Sousa Lourdes Helena Rodrigues Martins Vanusa Pousada da Hora Andrea Von Groll Micaele Quintana de Moura Maria Elisabeth Aires Berne Luciana Farias da Costa de Avila Carlos James Scaini 2021-01-01T00:00:00Z https://doi.org/10.1590/s1678-9946202163009 https://doaj.org/article/60d0e89aaca343729d6866b8d5da87f3 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652021000100206&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946202163009 https://doaj.org/article/60d0e89aaca343729d6866b8d5da87f3 Revista do Instituto de Medicina Tropical de São Paulo, Vol 63 (2021) Toxocariasis Probiotic Innate immunity Cytokine INF-γ Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.1590/s1678-9946202163009 2024-08-05T17:49:30Z ABSTRACT Human toxocariasis consists of chronic tissue parasitosis that is difficult to treat and control. This study aimed to evaluate the action of the probiotic Lactobacillus acidophilus ATCC 4356 on larvae of Toxocara canis and the effect of IFN-γ cytokine on parasite-host in vivo (1.109 CFU) and in vitro (1.106, 1.107, 1.108, 1.109 CFU) interactions. Four groups of six BALB/c mice were formed: G1 - L. acidophilus supplementation and T. canis infection; G2 - T. canis infection; G3 - L. acidophilus supplementation; and G4 - PBS administration. Mice were intragastrically suplemented with probiotics for 15 days before inoculation and 48 h after inoculation with 100 T. canis eggs. The inoculation of T. canis was also perfomed intragastrically. The recovery of larvae took place through digestion of liver and lung tissues; the evaluation of IFN-γ gene transcription in leukocytes was performed by qPCR. The in vitro test consisted of incubating the probiotic with T. canis larvae. The supplementation of probiotics produced a reduction of 57.7% (p = 0.025) in the intensity of infection of T. canis larvae in mice, whereas in the in vitro test, there was no larvicidal effect. In addition, a decrease in the IFN-γ gene transcription was observed in both, T. canis-infected and uninfected mice, regardless of whether or not they received supplementation. The probiotic L. acidophilus ATCC 4356 reduced T. canis infection intensity in mice, however, the probiotic did not have a direct effect on larvae, demonstrating the need of interaction with the host for the beneficial effect of the probiotic to occur. Yet, the proinflammatory cytokine IFN-γ did not apparently contributed to the observed beneficial effect of probiotics. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 63 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Toxocariasis Probiotic Innate immunity Cytokine INF-γ Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Toxocariasis Probiotic Innate immunity Cytokine INF-γ Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Priscila Silva Cadore Débora Liliane Walcher Nicholas Frota Gonçalves Correia de Sousa Lourdes Helena Rodrigues Martins Vanusa Pousada da Hora Andrea Von Groll Micaele Quintana de Moura Maria Elisabeth Aires Berne Luciana Farias da Costa de Avila Carlos James Scaini Protective effect of the probiotic Lactobacillus acidophilus ATCC 4356 in BALB/c mice infected with Toxocara canis |
topic_facet |
Toxocariasis Probiotic Innate immunity Cytokine INF-γ Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
ABSTRACT Human toxocariasis consists of chronic tissue parasitosis that is difficult to treat and control. This study aimed to evaluate the action of the probiotic Lactobacillus acidophilus ATCC 4356 on larvae of Toxocara canis and the effect of IFN-γ cytokine on parasite-host in vivo (1.109 CFU) and in vitro (1.106, 1.107, 1.108, 1.109 CFU) interactions. Four groups of six BALB/c mice were formed: G1 - L. acidophilus supplementation and T. canis infection; G2 - T. canis infection; G3 - L. acidophilus supplementation; and G4 - PBS administration. Mice were intragastrically suplemented with probiotics for 15 days before inoculation and 48 h after inoculation with 100 T. canis eggs. The inoculation of T. canis was also perfomed intragastrically. The recovery of larvae took place through digestion of liver and lung tissues; the evaluation of IFN-γ gene transcription in leukocytes was performed by qPCR. The in vitro test consisted of incubating the probiotic with T. canis larvae. The supplementation of probiotics produced a reduction of 57.7% (p = 0.025) in the intensity of infection of T. canis larvae in mice, whereas in the in vitro test, there was no larvicidal effect. In addition, a decrease in the IFN-γ gene transcription was observed in both, T. canis-infected and uninfected mice, regardless of whether or not they received supplementation. The probiotic L. acidophilus ATCC 4356 reduced T. canis infection intensity in mice, however, the probiotic did not have a direct effect on larvae, demonstrating the need of interaction with the host for the beneficial effect of the probiotic to occur. Yet, the proinflammatory cytokine IFN-γ did not apparently contributed to the observed beneficial effect of probiotics. |
format |
Article in Journal/Newspaper |
author |
Priscila Silva Cadore Débora Liliane Walcher Nicholas Frota Gonçalves Correia de Sousa Lourdes Helena Rodrigues Martins Vanusa Pousada da Hora Andrea Von Groll Micaele Quintana de Moura Maria Elisabeth Aires Berne Luciana Farias da Costa de Avila Carlos James Scaini |
author_facet |
Priscila Silva Cadore Débora Liliane Walcher Nicholas Frota Gonçalves Correia de Sousa Lourdes Helena Rodrigues Martins Vanusa Pousada da Hora Andrea Von Groll Micaele Quintana de Moura Maria Elisabeth Aires Berne Luciana Farias da Costa de Avila Carlos James Scaini |
author_sort |
Priscila Silva Cadore |
title |
Protective effect of the probiotic Lactobacillus acidophilus ATCC 4356 in BALB/c mice infected with Toxocara canis |
title_short |
Protective effect of the probiotic Lactobacillus acidophilus ATCC 4356 in BALB/c mice infected with Toxocara canis |
title_full |
Protective effect of the probiotic Lactobacillus acidophilus ATCC 4356 in BALB/c mice infected with Toxocara canis |
title_fullStr |
Protective effect of the probiotic Lactobacillus acidophilus ATCC 4356 in BALB/c mice infected with Toxocara canis |
title_full_unstemmed |
Protective effect of the probiotic Lactobacillus acidophilus ATCC 4356 in BALB/c mice infected with Toxocara canis |
title_sort |
protective effect of the probiotic lactobacillus acidophilus atcc 4356 in balb/c mice infected with toxocara canis |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2021 |
url |
https://doi.org/10.1590/s1678-9946202163009 https://doaj.org/article/60d0e89aaca343729d6866b8d5da87f3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 63 (2021) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652021000100206&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946202163009 https://doaj.org/article/60d0e89aaca343729d6866b8d5da87f3 |
op_doi |
https://doi.org/10.1590/s1678-9946202163009 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
63 |
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