Protective effect of the probiotic Lactobacillus acidophilus ATCC 4356 in BALB/c mice infected with Toxocara canis

ABSTRACT Human toxocariasis consists of chronic tissue parasitosis that is difficult to treat and control. This study aimed to evaluate the action of the probiotic Lactobacillus acidophilus ATCC 4356 on larvae of Toxocara canis and the effect of IFN-γ cytokine on parasite-host in vivo (1.109 CFU) an...

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Published in:Revista do Instituto de Medicina Tropical de São Paulo
Main Authors: Priscila Silva Cadore, Débora Liliane Walcher, Nicholas Frota Gonçalves Correia de Sousa, Lourdes Helena Rodrigues Martins, Vanusa Pousada da Hora, Andrea Von Groll, Micaele Quintana de Moura, Maria Elisabeth Aires Berne, Luciana Farias da Costa de Avila, Carlos James Scaini
Format: Article in Journal/Newspaper
Language:English
Published: Universidade de São Paulo (USP) 2021
Subjects:
Online Access:https://doi.org/10.1590/s1678-9946202163009
https://doaj.org/article/60d0e89aaca343729d6866b8d5da87f3
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spelling ftdoajarticles:oai:doaj.org/article:60d0e89aaca343729d6866b8d5da87f3 2024-09-09T19:27:29+00:00 Protective effect of the probiotic Lactobacillus acidophilus ATCC 4356 in BALB/c mice infected with Toxocara canis Priscila Silva Cadore Débora Liliane Walcher Nicholas Frota Gonçalves Correia de Sousa Lourdes Helena Rodrigues Martins Vanusa Pousada da Hora Andrea Von Groll Micaele Quintana de Moura Maria Elisabeth Aires Berne Luciana Farias da Costa de Avila Carlos James Scaini 2021-01-01T00:00:00Z https://doi.org/10.1590/s1678-9946202163009 https://doaj.org/article/60d0e89aaca343729d6866b8d5da87f3 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652021000100206&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946202163009 https://doaj.org/article/60d0e89aaca343729d6866b8d5da87f3 Revista do Instituto de Medicina Tropical de São Paulo, Vol 63 (2021) Toxocariasis Probiotic Innate immunity Cytokine INF-γ Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.1590/s1678-9946202163009 2024-08-05T17:49:30Z ABSTRACT Human toxocariasis consists of chronic tissue parasitosis that is difficult to treat and control. This study aimed to evaluate the action of the probiotic Lactobacillus acidophilus ATCC 4356 on larvae of Toxocara canis and the effect of IFN-γ cytokine on parasite-host in vivo (1.109 CFU) and in vitro (1.106, 1.107, 1.108, 1.109 CFU) interactions. Four groups of six BALB/c mice were formed: G1 - L. acidophilus supplementation and T. canis infection; G2 - T. canis infection; G3 - L. acidophilus supplementation; and G4 - PBS administration. Mice were intragastrically suplemented with probiotics for 15 days before inoculation and 48 h after inoculation with 100 T. canis eggs. The inoculation of T. canis was also perfomed intragastrically. The recovery of larvae took place through digestion of liver and lung tissues; the evaluation of IFN-γ gene transcription in leukocytes was performed by qPCR. The in vitro test consisted of incubating the probiotic with T. canis larvae. The supplementation of probiotics produced a reduction of 57.7% (p = 0.025) in the intensity of infection of T. canis larvae in mice, whereas in the in vitro test, there was no larvicidal effect. In addition, a decrease in the IFN-γ gene transcription was observed in both, T. canis-infected and uninfected mice, regardless of whether or not they received supplementation. The probiotic L. acidophilus ATCC 4356 reduced T. canis infection intensity in mice, however, the probiotic did not have a direct effect on larvae, demonstrating the need of interaction with the host for the beneficial effect of the probiotic to occur. Yet, the proinflammatory cytokine IFN-γ did not apparently contributed to the observed beneficial effect of probiotics. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 63
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic Toxocariasis
Probiotic
Innate immunity
Cytokine
INF-γ
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
spellingShingle Toxocariasis
Probiotic
Innate immunity
Cytokine
INF-γ
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Priscila Silva Cadore
Débora Liliane Walcher
Nicholas Frota Gonçalves Correia de Sousa
Lourdes Helena Rodrigues Martins
Vanusa Pousada da Hora
Andrea Von Groll
Micaele Quintana de Moura
Maria Elisabeth Aires Berne
Luciana Farias da Costa de Avila
Carlos James Scaini
Protective effect of the probiotic Lactobacillus acidophilus ATCC 4356 in BALB/c mice infected with Toxocara canis
topic_facet Toxocariasis
Probiotic
Innate immunity
Cytokine
INF-γ
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
description ABSTRACT Human toxocariasis consists of chronic tissue parasitosis that is difficult to treat and control. This study aimed to evaluate the action of the probiotic Lactobacillus acidophilus ATCC 4356 on larvae of Toxocara canis and the effect of IFN-γ cytokine on parasite-host in vivo (1.109 CFU) and in vitro (1.106, 1.107, 1.108, 1.109 CFU) interactions. Four groups of six BALB/c mice were formed: G1 - L. acidophilus supplementation and T. canis infection; G2 - T. canis infection; G3 - L. acidophilus supplementation; and G4 - PBS administration. Mice were intragastrically suplemented with probiotics for 15 days before inoculation and 48 h after inoculation with 100 T. canis eggs. The inoculation of T. canis was also perfomed intragastrically. The recovery of larvae took place through digestion of liver and lung tissues; the evaluation of IFN-γ gene transcription in leukocytes was performed by qPCR. The in vitro test consisted of incubating the probiotic with T. canis larvae. The supplementation of probiotics produced a reduction of 57.7% (p = 0.025) in the intensity of infection of T. canis larvae in mice, whereas in the in vitro test, there was no larvicidal effect. In addition, a decrease in the IFN-γ gene transcription was observed in both, T. canis-infected and uninfected mice, regardless of whether or not they received supplementation. The probiotic L. acidophilus ATCC 4356 reduced T. canis infection intensity in mice, however, the probiotic did not have a direct effect on larvae, demonstrating the need of interaction with the host for the beneficial effect of the probiotic to occur. Yet, the proinflammatory cytokine IFN-γ did not apparently contributed to the observed beneficial effect of probiotics.
format Article in Journal/Newspaper
author Priscila Silva Cadore
Débora Liliane Walcher
Nicholas Frota Gonçalves Correia de Sousa
Lourdes Helena Rodrigues Martins
Vanusa Pousada da Hora
Andrea Von Groll
Micaele Quintana de Moura
Maria Elisabeth Aires Berne
Luciana Farias da Costa de Avila
Carlos James Scaini
author_facet Priscila Silva Cadore
Débora Liliane Walcher
Nicholas Frota Gonçalves Correia de Sousa
Lourdes Helena Rodrigues Martins
Vanusa Pousada da Hora
Andrea Von Groll
Micaele Quintana de Moura
Maria Elisabeth Aires Berne
Luciana Farias da Costa de Avila
Carlos James Scaini
author_sort Priscila Silva Cadore
title Protective effect of the probiotic Lactobacillus acidophilus ATCC 4356 in BALB/c mice infected with Toxocara canis
title_short Protective effect of the probiotic Lactobacillus acidophilus ATCC 4356 in BALB/c mice infected with Toxocara canis
title_full Protective effect of the probiotic Lactobacillus acidophilus ATCC 4356 in BALB/c mice infected with Toxocara canis
title_fullStr Protective effect of the probiotic Lactobacillus acidophilus ATCC 4356 in BALB/c mice infected with Toxocara canis
title_full_unstemmed Protective effect of the probiotic Lactobacillus acidophilus ATCC 4356 in BALB/c mice infected with Toxocara canis
title_sort protective effect of the probiotic lactobacillus acidophilus atcc 4356 in balb/c mice infected with toxocara canis
publisher Universidade de São Paulo (USP)
publishDate 2021
url https://doi.org/10.1590/s1678-9946202163009
https://doaj.org/article/60d0e89aaca343729d6866b8d5da87f3
geographic Arctic
geographic_facet Arctic
genre Arctic
genre_facet Arctic
op_source Revista do Instituto de Medicina Tropical de São Paulo, Vol 63 (2021)
op_relation http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652021000100206&tlng=en
https://doaj.org/toc/1678-9946
1678-9946
doi:10.1590/s1678-9946202163009
https://doaj.org/article/60d0e89aaca343729d6866b8d5da87f3
op_doi https://doi.org/10.1590/s1678-9946202163009
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