Biogeography and genetic diversity of clinical isolates of Burkholderia pseudomallei in Sri Lanka.
Background Melioidosis is a potentially fatal infectious disease caused by Burkholderia pseudomallei and the disease is endemic in Southeast Asia and Northern Australia. It has been confirmed as endemic in Sri Lanka. Genomic epidemiology of B. pseudomallei in Sri Lanka is largely unexplored. This st...
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ftdoajarticles:oai:doaj.org/article:60c7c469081f4fb2b20bae6283199a78 2023-05-15T15:16:38+02:00 Biogeography and genetic diversity of clinical isolates of Burkholderia pseudomallei in Sri Lanka. Himali S Jayasinghearachchi Enoka M Corea Kumari I Jayaratne Regina A Fonseka Thilini A Muthugama Jayanthi Masakorala Ravija Yc Ramasinghe Aruna D De Silva 2021-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009917 https://doaj.org/article/60c7c469081f4fb2b20bae6283199a78 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009917 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009917 https://doaj.org/article/60c7c469081f4fb2b20bae6283199a78 PLoS Neglected Tropical Diseases, Vol 15, Iss 12, p e0009917 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009917 2022-12-31T11:15:27Z Background Melioidosis is a potentially fatal infectious disease caused by Burkholderia pseudomallei and the disease is endemic in Southeast Asia and Northern Australia. It has been confirmed as endemic in Sri Lanka. Genomic epidemiology of B. pseudomallei in Sri Lanka is largely unexplored. This study aims to determine the biogeography and genetic diversity of clinical isolates of B. pseudomallei and the phylogenetic and evolutionary relationship of Sri Lankan sequence types (STs) to those found in other endemic regions of Southeast Asia and Oceania. Methods The distribution of variably present genetic markers [Burkholderia intracellular motility A (bimA) gene variants bimABP/bimABM, filamentous hemagglutinin 3 (fhaB3), Yersinia-like fimbrial (YLF) and B. thailandensis-like flagellum and chemotaxis (BTFC) gene clusters and lipopolysaccharide O-antigen type A (LPS type A)] was examined among 310 strains. Multilocus sequence typing (MLST) was done for 84 clinical isolates. The phylogenetic and evolutionary relationship of Sri Lankan STs within Sri Lanka and in relation to those found in other endemic regions of Southeast Asia and Oceania were studied using e BURST, PHYLOViZ and minimum evolutionary analysis. Results The Sri Lankan B. pseudomallei population contained a large proportion of the rare BTFC clade (14.5%) and bimABM allele variant (18.5%) with differential geographic distribution. Genotypes fhaB3 and LPSA were found in 80% and 86% respectively. This study reported 43 STs (including 22 novel). e-BURST analysis which include all Sri Lankan STs (71) resulted in four groups, with a large clonal group (group 1) having 46 STs, and 17 singletons. ST1137 was the commonest ST. Several STs were shared with India, Bangladesh and Cambodia. Conclusion This study demonstrates the usefulness of high-resolution molecular typing to locate isolates within the broad geographical boundaries of B. pseudomallei at a global level and reveals that Sri Lankan isolates are intermediate between Southeast Asia and Oceania. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 12 e0009917 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Himali S Jayasinghearachchi Enoka M Corea Kumari I Jayaratne Regina A Fonseka Thilini A Muthugama Jayanthi Masakorala Ravija Yc Ramasinghe Aruna D De Silva Biogeography and genetic diversity of clinical isolates of Burkholderia pseudomallei in Sri Lanka. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Melioidosis is a potentially fatal infectious disease caused by Burkholderia pseudomallei and the disease is endemic in Southeast Asia and Northern Australia. It has been confirmed as endemic in Sri Lanka. Genomic epidemiology of B. pseudomallei in Sri Lanka is largely unexplored. This study aims to determine the biogeography and genetic diversity of clinical isolates of B. pseudomallei and the phylogenetic and evolutionary relationship of Sri Lankan sequence types (STs) to those found in other endemic regions of Southeast Asia and Oceania. Methods The distribution of variably present genetic markers [Burkholderia intracellular motility A (bimA) gene variants bimABP/bimABM, filamentous hemagglutinin 3 (fhaB3), Yersinia-like fimbrial (YLF) and B. thailandensis-like flagellum and chemotaxis (BTFC) gene clusters and lipopolysaccharide O-antigen type A (LPS type A)] was examined among 310 strains. Multilocus sequence typing (MLST) was done for 84 clinical isolates. The phylogenetic and evolutionary relationship of Sri Lankan STs within Sri Lanka and in relation to those found in other endemic regions of Southeast Asia and Oceania were studied using e BURST, PHYLOViZ and minimum evolutionary analysis. Results The Sri Lankan B. pseudomallei population contained a large proportion of the rare BTFC clade (14.5%) and bimABM allele variant (18.5%) with differential geographic distribution. Genotypes fhaB3 and LPSA were found in 80% and 86% respectively. This study reported 43 STs (including 22 novel). e-BURST analysis which include all Sri Lankan STs (71) resulted in four groups, with a large clonal group (group 1) having 46 STs, and 17 singletons. ST1137 was the commonest ST. Several STs were shared with India, Bangladesh and Cambodia. Conclusion This study demonstrates the usefulness of high-resolution molecular typing to locate isolates within the broad geographical boundaries of B. pseudomallei at a global level and reveals that Sri Lankan isolates are intermediate between Southeast Asia and Oceania. |
format |
Article in Journal/Newspaper |
author |
Himali S Jayasinghearachchi Enoka M Corea Kumari I Jayaratne Regina A Fonseka Thilini A Muthugama Jayanthi Masakorala Ravija Yc Ramasinghe Aruna D De Silva |
author_facet |
Himali S Jayasinghearachchi Enoka M Corea Kumari I Jayaratne Regina A Fonseka Thilini A Muthugama Jayanthi Masakorala Ravija Yc Ramasinghe Aruna D De Silva |
author_sort |
Himali S Jayasinghearachchi |
title |
Biogeography and genetic diversity of clinical isolates of Burkholderia pseudomallei in Sri Lanka. |
title_short |
Biogeography and genetic diversity of clinical isolates of Burkholderia pseudomallei in Sri Lanka. |
title_full |
Biogeography and genetic diversity of clinical isolates of Burkholderia pseudomallei in Sri Lanka. |
title_fullStr |
Biogeography and genetic diversity of clinical isolates of Burkholderia pseudomallei in Sri Lanka. |
title_full_unstemmed |
Biogeography and genetic diversity of clinical isolates of Burkholderia pseudomallei in Sri Lanka. |
title_sort |
biogeography and genetic diversity of clinical isolates of burkholderia pseudomallei in sri lanka. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009917 https://doaj.org/article/60c7c469081f4fb2b20bae6283199a78 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 12, p e0009917 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009917 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009917 https://doaj.org/article/60c7c469081f4fb2b20bae6283199a78 |
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https://doi.org/10.1371/journal.pntd.0009917 |
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PLOS Neglected Tropical Diseases |
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15 |
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12 |
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e0009917 |
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