Rickettsia parkeri: a Rickettsial pathogen transmitted by ticks in endemic areas for spotted fever rickettsiosis in southern Uruguay
At first Rickettsia conorii was implicated as the causative agent of spotted fever in Uruguay diagnosed by serological assays. Later Rickettsia parkeri was detected in human-biting Amblyomma triste ticks using molecular tests. The natural vector of R. conorii, Rhipicephalus sanguineus, has not been...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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Universidade de São Paulo (USP)
2012
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Online Access: | https://doi.org/10.1590/S0036-46652012000300003 https://doaj.org/article/6097c22fbfdc4fa4bd566a2ca5f6b0fd |
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ftdoajarticles:oai:doaj.org/article:6097c22fbfdc4fa4bd566a2ca5f6b0fd 2024-09-09T19:26:46+00:00 Rickettsia parkeri: a Rickettsial pathogen transmitted by ticks in endemic areas for spotted fever rickettsiosis in southern Uruguay José M. Venzal Agustín Estrada-Peña Aránzazu Portillo Atilio J. Mangold Oscar Castro Carlos G. De Souza María L. Félix Laura Pérez-Martínez Sonia Santibánez José A. Oteo 2012-06-01T00:00:00Z https://doi.org/10.1590/S0036-46652012000300003 https://doaj.org/article/6097c22fbfdc4fa4bd566a2ca5f6b0fd EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652012000300003&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652012000300003 https://doaj.org/article/6097c22fbfdc4fa4bd566a2ca5f6b0fd Revista do Instituto de Medicina Tropical de São Paulo, Vol 54, Iss 3, Pp 131-134 (2012) Rhipicephalus sanguineus Amblyomma triste Rickettsia parkeri Spotted fever group (SFG) Uruguay Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles https://doi.org/10.1590/S0036-46652012000300003 2024-08-05T17:49:31Z At first Rickettsia conorii was implicated as the causative agent of spotted fever in Uruguay diagnosed by serological assays. Later Rickettsia parkeri was detected in human-biting Amblyomma triste ticks using molecular tests. The natural vector of R. conorii, Rhipicephalus sanguineus, has not been studied for the presence of rickettsial organisms in Uruguay. To address this question, 180 R. sanguineus from dogs and 245 A. triste from vegetation (flagging) collected in three endemic localities were screened for spotted fever group (SFG) rickettsiosis in southern Uruguay. Tick extracted DNA pools were subjected to PCR using primers which amplify a fragment of the rickettsial gltA gene. Positive tick DNA pools with these primers were subjected to a second PCR round with primers targeting a fragment of the ompA gene, which is only present in SFG rickettsiae. No rickettsial DNA was detected in R. sanguineus. However, DNA pools of A. triste were found to be positive for a rickettsial organism in two of the three localities, with prevalences of 11.8% to 37.5% positive pools. DNA sequences generated from these PCR-positive ticks corresponded to R. parkeri. These findings, joint with the aggressiveness shown by A. triste towards humans, support previous data on the involvement of A. triste as vector of human infections caused by R. parkeri in Uruguay. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Uruguay Revista do Instituto de Medicina Tropical de São Paulo 54 3 131 134 |
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ftdoajarticles |
language |
English |
topic |
Rhipicephalus sanguineus Amblyomma triste Rickettsia parkeri Spotted fever group (SFG) Uruguay Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Rhipicephalus sanguineus Amblyomma triste Rickettsia parkeri Spotted fever group (SFG) Uruguay Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 José M. Venzal Agustín Estrada-Peña Aránzazu Portillo Atilio J. Mangold Oscar Castro Carlos G. De Souza María L. Félix Laura Pérez-Martínez Sonia Santibánez José A. Oteo Rickettsia parkeri: a Rickettsial pathogen transmitted by ticks in endemic areas for spotted fever rickettsiosis in southern Uruguay |
topic_facet |
Rhipicephalus sanguineus Amblyomma triste Rickettsia parkeri Spotted fever group (SFG) Uruguay Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
At first Rickettsia conorii was implicated as the causative agent of spotted fever in Uruguay diagnosed by serological assays. Later Rickettsia parkeri was detected in human-biting Amblyomma triste ticks using molecular tests. The natural vector of R. conorii, Rhipicephalus sanguineus, has not been studied for the presence of rickettsial organisms in Uruguay. To address this question, 180 R. sanguineus from dogs and 245 A. triste from vegetation (flagging) collected in three endemic localities were screened for spotted fever group (SFG) rickettsiosis in southern Uruguay. Tick extracted DNA pools were subjected to PCR using primers which amplify a fragment of the rickettsial gltA gene. Positive tick DNA pools with these primers were subjected to a second PCR round with primers targeting a fragment of the ompA gene, which is only present in SFG rickettsiae. No rickettsial DNA was detected in R. sanguineus. However, DNA pools of A. triste were found to be positive for a rickettsial organism in two of the three localities, with prevalences of 11.8% to 37.5% positive pools. DNA sequences generated from these PCR-positive ticks corresponded to R. parkeri. These findings, joint with the aggressiveness shown by A. triste towards humans, support previous data on the involvement of A. triste as vector of human infections caused by R. parkeri in Uruguay. |
format |
Article in Journal/Newspaper |
author |
José M. Venzal Agustín Estrada-Peña Aránzazu Portillo Atilio J. Mangold Oscar Castro Carlos G. De Souza María L. Félix Laura Pérez-Martínez Sonia Santibánez José A. Oteo |
author_facet |
José M. Venzal Agustín Estrada-Peña Aránzazu Portillo Atilio J. Mangold Oscar Castro Carlos G. De Souza María L. Félix Laura Pérez-Martínez Sonia Santibánez José A. Oteo |
author_sort |
José M. Venzal |
title |
Rickettsia parkeri: a Rickettsial pathogen transmitted by ticks in endemic areas for spotted fever rickettsiosis in southern Uruguay |
title_short |
Rickettsia parkeri: a Rickettsial pathogen transmitted by ticks in endemic areas for spotted fever rickettsiosis in southern Uruguay |
title_full |
Rickettsia parkeri: a Rickettsial pathogen transmitted by ticks in endemic areas for spotted fever rickettsiosis in southern Uruguay |
title_fullStr |
Rickettsia parkeri: a Rickettsial pathogen transmitted by ticks in endemic areas for spotted fever rickettsiosis in southern Uruguay |
title_full_unstemmed |
Rickettsia parkeri: a Rickettsial pathogen transmitted by ticks in endemic areas for spotted fever rickettsiosis in southern Uruguay |
title_sort |
rickettsia parkeri: a rickettsial pathogen transmitted by ticks in endemic areas for spotted fever rickettsiosis in southern uruguay |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2012 |
url |
https://doi.org/10.1590/S0036-46652012000300003 https://doaj.org/article/6097c22fbfdc4fa4bd566a2ca5f6b0fd |
geographic |
Arctic Uruguay |
geographic_facet |
Arctic Uruguay |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 54, Iss 3, Pp 131-134 (2012) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652012000300003&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652012000300003 https://doaj.org/article/6097c22fbfdc4fa4bd566a2ca5f6b0fd |
op_doi |
https://doi.org/10.1590/S0036-46652012000300003 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
54 |
container_issue |
3 |
container_start_page |
131 |
op_container_end_page |
134 |
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