Multilocus sequence based identification and adaptational strategies of Pseudomonas sp. from the supraglacial site of Sikkim Himalaya.
Microorganisms inhabiting the supraglacial ice are biotechnologically significant as they are equipped with unique adaptive features in response to extreme environmental conditions of high ultraviolet radiations and frequent freeze-thaw. In the current study, we obtained eleven strains of Pseudomona...
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ftdoajarticles:oai:doaj.org/article:6090b7c502774537bed7955dedaf1302 2023-05-15T13:40:35+02:00 Multilocus sequence based identification and adaptational strategies of Pseudomonas sp. from the supraglacial site of Sikkim Himalaya. Srijana Mukhia Anil Kumar Poonam Kumari Rakshak Kumar Sanjay Kumar 2022-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0261178 https://doaj.org/article/6090b7c502774537bed7955dedaf1302 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pone.0261178 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0261178 https://doaj.org/article/6090b7c502774537bed7955dedaf1302 PLoS ONE, Vol 17, Iss 1, p e0261178 (2022) Medicine R Science Q article 2022 ftdoajarticles https://doi.org/10.1371/journal.pone.0261178 2022-12-31T15:05:53Z Microorganisms inhabiting the supraglacial ice are biotechnologically significant as they are equipped with unique adaptive features in response to extreme environmental conditions of high ultraviolet radiations and frequent freeze-thaw. In the current study, we obtained eleven strains of Pseudomonas from the East Rathong supraglacial site in Sikkim Himalaya that showed taxonomic ambiguity in terms of species affiliation. Being one of the most complex and diverse genera, deciphering the correct taxonomy of Pseudomonas species has always been challenging. So, we conducted multilocus sequence analysis (MLSA) using five housekeeping genes, which concluded the taxonomic assignment of these strains to Pseudomonas antarctica. This was further supported by the lesser mean genetic distances with P. antarctica (0.73%) compared to P. fluorescens (3.65%), and highest ANI value of ~99 and dDDH value of 91.2 of the representative strains with P. antarctica PAMC 27494. We examined the multi-tolerance abilities of these eleven Pseudomonas strains. Indeed the studied strains displayed significant tolerance to freezing for 96 hours compared to the mesophilic control strain, while except for four strains, seven strains exhibited noteworthy tolerance to UV-C radiations. The genome-based findings revealed many cold and radiation resistance-associated genes that supported the physiological findings. Further, the bacterial strains produced two or more cold-active enzymes in plate-based assays. Owing to the polyadaptational attributes, the strains ERGC3:01 and ERGC3:05 could be most promising for bioprospection. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles PLOS ONE 17 1 e0261178 |
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Medicine R Science Q |
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Medicine R Science Q Srijana Mukhia Anil Kumar Poonam Kumari Rakshak Kumar Sanjay Kumar Multilocus sequence based identification and adaptational strategies of Pseudomonas sp. from the supraglacial site of Sikkim Himalaya. |
topic_facet |
Medicine R Science Q |
description |
Microorganisms inhabiting the supraglacial ice are biotechnologically significant as they are equipped with unique adaptive features in response to extreme environmental conditions of high ultraviolet radiations and frequent freeze-thaw. In the current study, we obtained eleven strains of Pseudomonas from the East Rathong supraglacial site in Sikkim Himalaya that showed taxonomic ambiguity in terms of species affiliation. Being one of the most complex and diverse genera, deciphering the correct taxonomy of Pseudomonas species has always been challenging. So, we conducted multilocus sequence analysis (MLSA) using five housekeeping genes, which concluded the taxonomic assignment of these strains to Pseudomonas antarctica. This was further supported by the lesser mean genetic distances with P. antarctica (0.73%) compared to P. fluorescens (3.65%), and highest ANI value of ~99 and dDDH value of 91.2 of the representative strains with P. antarctica PAMC 27494. We examined the multi-tolerance abilities of these eleven Pseudomonas strains. Indeed the studied strains displayed significant tolerance to freezing for 96 hours compared to the mesophilic control strain, while except for four strains, seven strains exhibited noteworthy tolerance to UV-C radiations. The genome-based findings revealed many cold and radiation resistance-associated genes that supported the physiological findings. Further, the bacterial strains produced two or more cold-active enzymes in plate-based assays. Owing to the polyadaptational attributes, the strains ERGC3:01 and ERGC3:05 could be most promising for bioprospection. |
format |
Article in Journal/Newspaper |
author |
Srijana Mukhia Anil Kumar Poonam Kumari Rakshak Kumar Sanjay Kumar |
author_facet |
Srijana Mukhia Anil Kumar Poonam Kumari Rakshak Kumar Sanjay Kumar |
author_sort |
Srijana Mukhia |
title |
Multilocus sequence based identification and adaptational strategies of Pseudomonas sp. from the supraglacial site of Sikkim Himalaya. |
title_short |
Multilocus sequence based identification and adaptational strategies of Pseudomonas sp. from the supraglacial site of Sikkim Himalaya. |
title_full |
Multilocus sequence based identification and adaptational strategies of Pseudomonas sp. from the supraglacial site of Sikkim Himalaya. |
title_fullStr |
Multilocus sequence based identification and adaptational strategies of Pseudomonas sp. from the supraglacial site of Sikkim Himalaya. |
title_full_unstemmed |
Multilocus sequence based identification and adaptational strategies of Pseudomonas sp. from the supraglacial site of Sikkim Himalaya. |
title_sort |
multilocus sequence based identification and adaptational strategies of pseudomonas sp. from the supraglacial site of sikkim himalaya. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pone.0261178 https://doaj.org/article/6090b7c502774537bed7955dedaf1302 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
PLoS ONE, Vol 17, Iss 1, p e0261178 (2022) |
op_relation |
https://doi.org/10.1371/journal.pone.0261178 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0261178 https://doaj.org/article/6090b7c502774537bed7955dedaf1302 |
op_doi |
https://doi.org/10.1371/journal.pone.0261178 |
container_title |
PLOS ONE |
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17 |
container_issue |
1 |
container_start_page |
e0261178 |
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1766137337535791104 |