Multilocus detection of wolf x dog hybridization in italy, and guidelines for marker selection.
Hybridization and introgression can impact the evolution of natural populations. Several wild canid species hybridize in nature, sometimes originating new taxa. However, hybridization with free-ranging dogs is threatening the genetic integrity of grey wolf populations (Canis lupus), or even the surv...
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ftdoajarticles:oai:doaj.org/article:606a7d050f404029a8cc0976ef888cd2 2023-05-15T15:51:09+02:00 Multilocus detection of wolf x dog hybridization in italy, and guidelines for marker selection. Ettore Randi Pavel Hulva Elena Fabbri Marco Galaverni Ana Galov Josip Kusak Daniele Bigi Barbora Černá Bolfíková Milena Smetanová Romolo Caniglia 2014-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0086409 https://doaj.org/article/606a7d050f404029a8cc0976ef888cd2 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3899229?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0086409 https://doaj.org/article/606a7d050f404029a8cc0976ef888cd2 PLoS ONE, Vol 9, Iss 1, p e86409 (2014) Medicine R Science Q article 2014 ftdoajarticles https://doi.org/10.1371/journal.pone.0086409 2022-12-31T13:05:54Z Hybridization and introgression can impact the evolution of natural populations. Several wild canid species hybridize in nature, sometimes originating new taxa. However, hybridization with free-ranging dogs is threatening the genetic integrity of grey wolf populations (Canis lupus), or even the survival of endangered species (e.g., the Ethiopian wolf C. simensis). Efficient molecular tools to assess hybridization rates are essential in wolf conservation strategies. We evaluated the power of biparental and uniparental markers (39 autosomal and 4 Y-linked microsatellites, a melanistic deletion at the β-defensin CBD103 gene, the hypervariable domain of the mtDNA control-region) to identify the multilocus admixture patterns in wolf x dog hybrids. We used empirical data from 2 hybrid groups with different histories: 30 presumptive natural hybrids from Italy and 73 Czechoslovakian wolfdogs of known hybrid origin, as well as simulated data. We assessed the efficiency of various marker combinations and reference samples in admixture analyses using 69 dogs of different breeds and 99 wolves from Italy, Balkans and Carpathian Mountains. Results confirmed the occurrence of hybrids in Italy, some of them showing anomalous phenotypic traits and exogenous mtDNA or Y-chromosome introgression. Hybridization was mostly attributable to village dogs and not strictly patrilineal. The melanistic β-defensin deletion was found only in Italian dogs and in putative hybrids. The 24 most divergent microsatellites (largest wolf-dog FST values) were equally or more informative than the entire panel of 39 loci. A smaller panel of 12 microsatellites increased risks to identify false admixed individuals. The frequency of F1 and F2 was lower than backcrosses or introgressed individuals, suggesting hybridization already occurred some generations in the past, during early phases of wolf expansion from their historical core areas. Empirical and simulated data indicated the identification of the past generation backcrosses is always uncertain, and a ... Article in Journal/Newspaper Canis lupus Directory of Open Access Journals: DOAJ Articles PLoS ONE 9 1 e86409 |
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Medicine R Science Q |
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Medicine R Science Q Ettore Randi Pavel Hulva Elena Fabbri Marco Galaverni Ana Galov Josip Kusak Daniele Bigi Barbora Černá Bolfíková Milena Smetanová Romolo Caniglia Multilocus detection of wolf x dog hybridization in italy, and guidelines for marker selection. |
topic_facet |
Medicine R Science Q |
description |
Hybridization and introgression can impact the evolution of natural populations. Several wild canid species hybridize in nature, sometimes originating new taxa. However, hybridization with free-ranging dogs is threatening the genetic integrity of grey wolf populations (Canis lupus), or even the survival of endangered species (e.g., the Ethiopian wolf C. simensis). Efficient molecular tools to assess hybridization rates are essential in wolf conservation strategies. We evaluated the power of biparental and uniparental markers (39 autosomal and 4 Y-linked microsatellites, a melanistic deletion at the β-defensin CBD103 gene, the hypervariable domain of the mtDNA control-region) to identify the multilocus admixture patterns in wolf x dog hybrids. We used empirical data from 2 hybrid groups with different histories: 30 presumptive natural hybrids from Italy and 73 Czechoslovakian wolfdogs of known hybrid origin, as well as simulated data. We assessed the efficiency of various marker combinations and reference samples in admixture analyses using 69 dogs of different breeds and 99 wolves from Italy, Balkans and Carpathian Mountains. Results confirmed the occurrence of hybrids in Italy, some of them showing anomalous phenotypic traits and exogenous mtDNA or Y-chromosome introgression. Hybridization was mostly attributable to village dogs and not strictly patrilineal. The melanistic β-defensin deletion was found only in Italian dogs and in putative hybrids. The 24 most divergent microsatellites (largest wolf-dog FST values) were equally or more informative than the entire panel of 39 loci. A smaller panel of 12 microsatellites increased risks to identify false admixed individuals. The frequency of F1 and F2 was lower than backcrosses or introgressed individuals, suggesting hybridization already occurred some generations in the past, during early phases of wolf expansion from their historical core areas. Empirical and simulated data indicated the identification of the past generation backcrosses is always uncertain, and a ... |
format |
Article in Journal/Newspaper |
author |
Ettore Randi Pavel Hulva Elena Fabbri Marco Galaverni Ana Galov Josip Kusak Daniele Bigi Barbora Černá Bolfíková Milena Smetanová Romolo Caniglia |
author_facet |
Ettore Randi Pavel Hulva Elena Fabbri Marco Galaverni Ana Galov Josip Kusak Daniele Bigi Barbora Černá Bolfíková Milena Smetanová Romolo Caniglia |
author_sort |
Ettore Randi |
title |
Multilocus detection of wolf x dog hybridization in italy, and guidelines for marker selection. |
title_short |
Multilocus detection of wolf x dog hybridization in italy, and guidelines for marker selection. |
title_full |
Multilocus detection of wolf x dog hybridization in italy, and guidelines for marker selection. |
title_fullStr |
Multilocus detection of wolf x dog hybridization in italy, and guidelines for marker selection. |
title_full_unstemmed |
Multilocus detection of wolf x dog hybridization in italy, and guidelines for marker selection. |
title_sort |
multilocus detection of wolf x dog hybridization in italy, and guidelines for marker selection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pone.0086409 https://doaj.org/article/606a7d050f404029a8cc0976ef888cd2 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
PLoS ONE, Vol 9, Iss 1, p e86409 (2014) |
op_relation |
http://europepmc.org/articles/PMC3899229?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0086409 https://doaj.org/article/606a7d050f404029a8cc0976ef888cd2 |
op_doi |
https://doi.org/10.1371/journal.pone.0086409 |
container_title |
PLoS ONE |
container_volume |
9 |
container_issue |
1 |
container_start_page |
e86409 |
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1766386209422049280 |