Exploring Vitamin B1 Cycling and Its Connections to the Microbial Community in the North Atlantic Ocean

Vitamin B1 (thiamin) is an essential coenzyme for all cells. Recent findings from experimental cell biology and genome surveys have shown that thiamin cycling by plankton is far more complex than was previously understood. Many plankton cells cannot produce thiamin (are auxotrophic) and obligately r...

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Published in:Frontiers in Marine Science
Main Authors: Christopher P. Suffridge, Luis M. Bolaños, Kristin Bergauer, Alexandra Z. Worden, Jeff Morré, Michael J. Behrenfeld, Stephen J. Giovannoni
Format: Article in Journal/Newspaper
Language:English
Published: Frontiers Media S.A. 2020
Subjects:
Q
Online Access:https://doi.org/10.3389/fmars.2020.606342
https://doaj.org/article/6040135de20e4951a24f973ec8ece8f2
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spelling ftdoajarticles:oai:doaj.org/article:6040135de20e4951a24f973ec8ece8f2 2023-05-15T17:32:03+02:00 Exploring Vitamin B1 Cycling and Its Connections to the Microbial Community in the North Atlantic Ocean Christopher P. Suffridge Luis M. Bolaños Kristin Bergauer Alexandra Z. Worden Jeff Morré Michael J. Behrenfeld Stephen J. Giovannoni 2020-12-01T00:00:00Z https://doi.org/10.3389/fmars.2020.606342 https://doaj.org/article/6040135de20e4951a24f973ec8ece8f2 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmars.2020.606342/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2020.606342 https://doaj.org/article/6040135de20e4951a24f973ec8ece8f2 Frontiers in Marine Science, Vol 7 (2020) thiamin B-vitamin bacterioplankton phytoplankton microbial interaction Science Q General. Including nature conservation geographical distribution QH1-199.5 article 2020 ftdoajarticles https://doi.org/10.3389/fmars.2020.606342 2022-12-31T07:47:08Z Vitamin B1 (thiamin) is an essential coenzyme for all cells. Recent findings from experimental cell biology and genome surveys have shown that thiamin cycling by plankton is far more complex than was previously understood. Many plankton cells cannot produce thiamin (are auxotrophic) and obligately require an exogenous source of thiamin or one or more of 5 different thiamin-related compounds (TRCs). Despite this emerging evidence for the evolution among plankton of complex interactions related to thiamin, the influence of TRCs on plankton community structure and productivity are not understood. We report measurements of three dissolved TRCs 4-amino-5-aminomethyl-2-methylpyrimidine (AmMP), 5-(2-hydroxyethyl)-4-methyl-1,3-thiazole-2-carboxylic acid (cHET), and 4-methyl-5-thiazoleethanol (HET) that have never before been assayed in seawater. Here we characterize them alongside other TRCs that were measured previously [thiamin and 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP)], in depth profiles from a latitudinal transect in the north Atlantic in March 2018. TRC concentrations ranged from femptomolar to picomolar. Surface depletion relative to a maximum near the bottom of the euphotic zone and low concentrations at deeper depths were consistent features. Our observations suggest that when bacterial abundance and production are low, TRC concentrations approach a steady state where TRC production and consumption terms are balanced. Standing stocks of TRCs also appear to be positively correlated with bacterial production. However, near the period of peak biomass in the accumulation phase of a bloom we observed an inverse relationship between TRCs and bacterial production, coincident with an increased abundance of Flavobacteria that comparative genomics indicates could be vitamin B1 auxotrophs. While these observations suggest that the dissolved pool of TRCs is often at steady state, with TRC production and consumption balanced, our data suggests that bloom induced shifts in microbial community structure and activity ... Article in Journal/Newspaper North Atlantic Directory of Open Access Journals: DOAJ Articles Frontiers in Marine Science 7
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic thiamin
B-vitamin
bacterioplankton
phytoplankton
microbial interaction
Science
Q
General. Including nature conservation
geographical distribution
QH1-199.5
spellingShingle thiamin
B-vitamin
bacterioplankton
phytoplankton
microbial interaction
Science
Q
General. Including nature conservation
geographical distribution
QH1-199.5
Christopher P. Suffridge
Luis M. Bolaños
Kristin Bergauer
Alexandra Z. Worden
Jeff Morré
Michael J. Behrenfeld
Stephen J. Giovannoni
Exploring Vitamin B1 Cycling and Its Connections to the Microbial Community in the North Atlantic Ocean
topic_facet thiamin
B-vitamin
bacterioplankton
phytoplankton
microbial interaction
Science
Q
General. Including nature conservation
geographical distribution
QH1-199.5
description Vitamin B1 (thiamin) is an essential coenzyme for all cells. Recent findings from experimental cell biology and genome surveys have shown that thiamin cycling by plankton is far more complex than was previously understood. Many plankton cells cannot produce thiamin (are auxotrophic) and obligately require an exogenous source of thiamin or one or more of 5 different thiamin-related compounds (TRCs). Despite this emerging evidence for the evolution among plankton of complex interactions related to thiamin, the influence of TRCs on plankton community structure and productivity are not understood. We report measurements of three dissolved TRCs 4-amino-5-aminomethyl-2-methylpyrimidine (AmMP), 5-(2-hydroxyethyl)-4-methyl-1,3-thiazole-2-carboxylic acid (cHET), and 4-methyl-5-thiazoleethanol (HET) that have never before been assayed in seawater. Here we characterize them alongside other TRCs that were measured previously [thiamin and 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP)], in depth profiles from a latitudinal transect in the north Atlantic in March 2018. TRC concentrations ranged from femptomolar to picomolar. Surface depletion relative to a maximum near the bottom of the euphotic zone and low concentrations at deeper depths were consistent features. Our observations suggest that when bacterial abundance and production are low, TRC concentrations approach a steady state where TRC production and consumption terms are balanced. Standing stocks of TRCs also appear to be positively correlated with bacterial production. However, near the period of peak biomass in the accumulation phase of a bloom we observed an inverse relationship between TRCs and bacterial production, coincident with an increased abundance of Flavobacteria that comparative genomics indicates could be vitamin B1 auxotrophs. While these observations suggest that the dissolved pool of TRCs is often at steady state, with TRC production and consumption balanced, our data suggests that bloom induced shifts in microbial community structure and activity ...
format Article in Journal/Newspaper
author Christopher P. Suffridge
Luis M. Bolaños
Kristin Bergauer
Alexandra Z. Worden
Jeff Morré
Michael J. Behrenfeld
Stephen J. Giovannoni
author_facet Christopher P. Suffridge
Luis M. Bolaños
Kristin Bergauer
Alexandra Z. Worden
Jeff Morré
Michael J. Behrenfeld
Stephen J. Giovannoni
author_sort Christopher P. Suffridge
title Exploring Vitamin B1 Cycling and Its Connections to the Microbial Community in the North Atlantic Ocean
title_short Exploring Vitamin B1 Cycling and Its Connections to the Microbial Community in the North Atlantic Ocean
title_full Exploring Vitamin B1 Cycling and Its Connections to the Microbial Community in the North Atlantic Ocean
title_fullStr Exploring Vitamin B1 Cycling and Its Connections to the Microbial Community in the North Atlantic Ocean
title_full_unstemmed Exploring Vitamin B1 Cycling and Its Connections to the Microbial Community in the North Atlantic Ocean
title_sort exploring vitamin b1 cycling and its connections to the microbial community in the north atlantic ocean
publisher Frontiers Media S.A.
publishDate 2020
url https://doi.org/10.3389/fmars.2020.606342
https://doaj.org/article/6040135de20e4951a24f973ec8ece8f2
genre North Atlantic
genre_facet North Atlantic
op_source Frontiers in Marine Science, Vol 7 (2020)
op_relation https://www.frontiersin.org/articles/10.3389/fmars.2020.606342/full
https://doaj.org/toc/2296-7745
2296-7745
doi:10.3389/fmars.2020.606342
https://doaj.org/article/6040135de20e4951a24f973ec8ece8f2
op_doi https://doi.org/10.3389/fmars.2020.606342
container_title Frontiers in Marine Science
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