Environmental Conditions Affecting the Expression of the SuoM Gene: An Stress Sigma Factor in M. smegmatis
Sigma factors associate with the RNA polymerases and confer specificity in the recognition of promoter regions. This provides a transcriptional regulatory level, that determines that a sets of genes is expressed according to the physiological necessities of bacterial cell. The sequence analysis of t...
Main Authors: | , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English Spanish |
Published: |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2002
|
Subjects: | |
Online Access: | https://doaj.org/article/601d0915d37d48b09aaadfc16618b92d |
id |
ftdoajarticles:oai:doaj.org/article:601d0915d37d48b09aaadfc16618b92d |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:601d0915d37d48b09aaadfc16618b92d 2023-10-01T03:54:12+02:00 Environmental Conditions Affecting the Expression of the SuoM Gene: An Stress Sigma Factor in M. smegmatis Nailet Arráiz L Salazar H Takiff 2002-11-01T00:00:00Z https://doaj.org/article/601d0915d37d48b09aaadfc16618b92d EN ES eng spa Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales https://produccioncientificaluz.org/index.php/kasmera/article/view/4698 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/601d0915d37d48b09aaadfc16618b92d Kasmera, Vol 30, Iss 2, Pp 101-111 (2002) M. smegmatis suoM estrés fase estacionaria B-galactosidasa pJEM15 Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2002 ftdoajarticles 2023-09-03T00:34:54Z Sigma factors associate with the RNA polymerases and confer specificity in the recognition of promoter regions. This provides a transcriptional regulatory level, that determines that a sets of genes is expressed according to the physiological necessities of bacterial cell. The sequence analysis of the region of replication origin of Mycobacterium smegmatis revealed the presence of a reading frame with capacity to codify a sigma factor of the ECF subfamily (“extracytoplasmic function”), which are characterized by directing the transcription of genes in response to changes in the environmental conditions. Transcriptional fusions PsuoM-lacZ’ in operon fusion plasmid pJEM15 were constructed with the objective of identifying the environmental conditions that stimulate the expression of the isolated gene, named suoM (“sigma linked to the origin in mycobacteria”), The constructions were introduced by electro-poration in M. smegmatis and the cells were grown under different environmental stress conditions. The promoter activity of the gene suoM was measured by B-galactosidasa expression. An increase of 2.0-3.5 times in the promoter activity was observed when the cells were transferred to 45°C and when the culture reached stationary growth phase. The results suggest that suoM could be involved in the regulation of gene expression in conditions of high temperature and in the stationary growth phase, inductive conditions that could have some significance in the pathogenic potential of mycobacteria and the ability of M. tuberculosis to cause latent infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Spanish |
topic |
M. smegmatis suoM estrés fase estacionaria B-galactosidasa pJEM15 Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
M. smegmatis suoM estrés fase estacionaria B-galactosidasa pJEM15 Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Nailet Arráiz L Salazar H Takiff Environmental Conditions Affecting the Expression of the SuoM Gene: An Stress Sigma Factor in M. smegmatis |
topic_facet |
M. smegmatis suoM estrés fase estacionaria B-galactosidasa pJEM15 Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Sigma factors associate with the RNA polymerases and confer specificity in the recognition of promoter regions. This provides a transcriptional regulatory level, that determines that a sets of genes is expressed according to the physiological necessities of bacterial cell. The sequence analysis of the region of replication origin of Mycobacterium smegmatis revealed the presence of a reading frame with capacity to codify a sigma factor of the ECF subfamily (“extracytoplasmic function”), which are characterized by directing the transcription of genes in response to changes in the environmental conditions. Transcriptional fusions PsuoM-lacZ’ in operon fusion plasmid pJEM15 were constructed with the objective of identifying the environmental conditions that stimulate the expression of the isolated gene, named suoM (“sigma linked to the origin in mycobacteria”), The constructions were introduced by electro-poration in M. smegmatis and the cells were grown under different environmental stress conditions. The promoter activity of the gene suoM was measured by B-galactosidasa expression. An increase of 2.0-3.5 times in the promoter activity was observed when the cells were transferred to 45°C and when the culture reached stationary growth phase. The results suggest that suoM could be involved in the regulation of gene expression in conditions of high temperature and in the stationary growth phase, inductive conditions that could have some significance in the pathogenic potential of mycobacteria and the ability of M. tuberculosis to cause latent infection. |
format |
Article in Journal/Newspaper |
author |
Nailet Arráiz L Salazar H Takiff |
author_facet |
Nailet Arráiz L Salazar H Takiff |
author_sort |
Nailet Arráiz |
title |
Environmental Conditions Affecting the Expression of the SuoM Gene: An Stress Sigma Factor in M. smegmatis |
title_short |
Environmental Conditions Affecting the Expression of the SuoM Gene: An Stress Sigma Factor in M. smegmatis |
title_full |
Environmental Conditions Affecting the Expression of the SuoM Gene: An Stress Sigma Factor in M. smegmatis |
title_fullStr |
Environmental Conditions Affecting the Expression of the SuoM Gene: An Stress Sigma Factor in M. smegmatis |
title_full_unstemmed |
Environmental Conditions Affecting the Expression of the SuoM Gene: An Stress Sigma Factor in M. smegmatis |
title_sort |
environmental conditions affecting the expression of the suom gene: an stress sigma factor in m. smegmatis |
publisher |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
publishDate |
2002 |
url |
https://doaj.org/article/601d0915d37d48b09aaadfc16618b92d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Kasmera, Vol 30, Iss 2, Pp 101-111 (2002) |
op_relation |
https://produccioncientificaluz.org/index.php/kasmera/article/view/4698 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/601d0915d37d48b09aaadfc16618b92d |
_version_ |
1778521608461221888 |