Characterization of Terrihabitans soli gen. nov., sp. nov., a Novel 0.2 μm-Filterable Soil Bacterium Belonging to a Widely Distributed Lineage of Hyphomicrobiales ( Rhizobiales )
We previously showed that novel filterable bacteria remain in “sterile” (<0.2 μm filtered) terrestrial environmental samples from Japan, China, and Arctic Norway. Here, we characterized the novel filterable strain IZ6 T , a representative strain of a widely distributed lineage. Phylogenetic analy...
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MDPI AG
2021
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ftdoajarticles:oai:doaj.org/article:5ff9c39acc5a4d16be4661e1be2392c5 2023-05-15T15:09:33+02:00 Characterization of Terrihabitans soli gen. nov., sp. nov., a Novel 0.2 μm-Filterable Soil Bacterium Belonging to a Widely Distributed Lineage of Hyphomicrobiales ( Rhizobiales ) Ryosuke Nakai Takeshi Naganuma Nozomi Tazato Tadao Kunihiro Sho Morohoshi Tomomi Koide Hiroyuki Kusada Hideyuki Tamaki Takashi Narihiro 2021-09-01T00:00:00Z https://doi.org/10.3390/d13090422 https://doaj.org/article/5ff9c39acc5a4d16be4661e1be2392c5 EN eng MDPI AG https://www.mdpi.com/1424-2818/13/9/422 https://doaj.org/toc/1424-2818 doi:10.3390/d13090422 1424-2818 https://doaj.org/article/5ff9c39acc5a4d16be4661e1be2392c5 Diversity, Vol 13, Iss 422, p 422 (2021) filter sterilization filterable bacteria soil phylogeny taxonomy Hyphomicrobiales Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.3390/d13090422 2022-12-30T23:42:07Z We previously showed that novel filterable bacteria remain in “sterile” (<0.2 μm filtered) terrestrial environmental samples from Japan, China, and Arctic Norway. Here, we characterized the novel filterable strain IZ6 T , a representative strain of a widely distributed lineage. Phylogenetic analysis showed that this strain was affiliated with the Rhizobiales (now proposed as Hyphomicrobiales ) of Alphaproteobacteria , but distinct from any other type strains. Strain IZ6 T shared the following chemotaxonomic features with the closest (but distantly) related type strain, Flaviflagellibacter deserti SYSU D60017 T : ubiquinone-10 as the major quinone; phosphatidylethanolamine, phosphatidylcholine, and phosphatidylglycerol as major polar lipids; and slightly high G+C content of 62.2 mol%. However, the cellular fatty acid composition differed between them, and the unsaturated fatty acid (C 18:1 ω 7 c /C 18:1 ω 6 c ) was predominantly found in our strain. Moreover, unlike methyrotrophs and nitrogen-fixers of the neighboring genera of Hyphomicrobiales ( Rhizobiales ), strain IZ6 T cannot utilize a one-carbon compound (e.g., methanol) and fix atmospheric nitrogen gas. These findings were consistent with the genome-inferred physiological potential. Based on the phylogenetic, physiological, and chemotaxonomic traits, we propose that strain IZ6 T represents a novel genus and species with the name Terrihabitans soli gen. nov., sp. nov. (=NBRC 106741 T = NCIMB 15058 T ). The findings will provide deeper insight into the eco-physiology of filterable microorganisms. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Norway Diversity 13 9 422 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
filter sterilization filterable bacteria soil phylogeny taxonomy Hyphomicrobiales Biology (General) QH301-705.5 |
spellingShingle |
filter sterilization filterable bacteria soil phylogeny taxonomy Hyphomicrobiales Biology (General) QH301-705.5 Ryosuke Nakai Takeshi Naganuma Nozomi Tazato Tadao Kunihiro Sho Morohoshi Tomomi Koide Hiroyuki Kusada Hideyuki Tamaki Takashi Narihiro Characterization of Terrihabitans soli gen. nov., sp. nov., a Novel 0.2 μm-Filterable Soil Bacterium Belonging to a Widely Distributed Lineage of Hyphomicrobiales ( Rhizobiales ) |
topic_facet |
filter sterilization filterable bacteria soil phylogeny taxonomy Hyphomicrobiales Biology (General) QH301-705.5 |
description |
We previously showed that novel filterable bacteria remain in “sterile” (<0.2 μm filtered) terrestrial environmental samples from Japan, China, and Arctic Norway. Here, we characterized the novel filterable strain IZ6 T , a representative strain of a widely distributed lineage. Phylogenetic analysis showed that this strain was affiliated with the Rhizobiales (now proposed as Hyphomicrobiales ) of Alphaproteobacteria , but distinct from any other type strains. Strain IZ6 T shared the following chemotaxonomic features with the closest (but distantly) related type strain, Flaviflagellibacter deserti SYSU D60017 T : ubiquinone-10 as the major quinone; phosphatidylethanolamine, phosphatidylcholine, and phosphatidylglycerol as major polar lipids; and slightly high G+C content of 62.2 mol%. However, the cellular fatty acid composition differed between them, and the unsaturated fatty acid (C 18:1 ω 7 c /C 18:1 ω 6 c ) was predominantly found in our strain. Moreover, unlike methyrotrophs and nitrogen-fixers of the neighboring genera of Hyphomicrobiales ( Rhizobiales ), strain IZ6 T cannot utilize a one-carbon compound (e.g., methanol) and fix atmospheric nitrogen gas. These findings were consistent with the genome-inferred physiological potential. Based on the phylogenetic, physiological, and chemotaxonomic traits, we propose that strain IZ6 T represents a novel genus and species with the name Terrihabitans soli gen. nov., sp. nov. (=NBRC 106741 T = NCIMB 15058 T ). The findings will provide deeper insight into the eco-physiology of filterable microorganisms. |
format |
Article in Journal/Newspaper |
author |
Ryosuke Nakai Takeshi Naganuma Nozomi Tazato Tadao Kunihiro Sho Morohoshi Tomomi Koide Hiroyuki Kusada Hideyuki Tamaki Takashi Narihiro |
author_facet |
Ryosuke Nakai Takeshi Naganuma Nozomi Tazato Tadao Kunihiro Sho Morohoshi Tomomi Koide Hiroyuki Kusada Hideyuki Tamaki Takashi Narihiro |
author_sort |
Ryosuke Nakai |
title |
Characterization of Terrihabitans soli gen. nov., sp. nov., a Novel 0.2 μm-Filterable Soil Bacterium Belonging to a Widely Distributed Lineage of Hyphomicrobiales ( Rhizobiales ) |
title_short |
Characterization of Terrihabitans soli gen. nov., sp. nov., a Novel 0.2 μm-Filterable Soil Bacterium Belonging to a Widely Distributed Lineage of Hyphomicrobiales ( Rhizobiales ) |
title_full |
Characterization of Terrihabitans soli gen. nov., sp. nov., a Novel 0.2 μm-Filterable Soil Bacterium Belonging to a Widely Distributed Lineage of Hyphomicrobiales ( Rhizobiales ) |
title_fullStr |
Characterization of Terrihabitans soli gen. nov., sp. nov., a Novel 0.2 μm-Filterable Soil Bacterium Belonging to a Widely Distributed Lineage of Hyphomicrobiales ( Rhizobiales ) |
title_full_unstemmed |
Characterization of Terrihabitans soli gen. nov., sp. nov., a Novel 0.2 μm-Filterable Soil Bacterium Belonging to a Widely Distributed Lineage of Hyphomicrobiales ( Rhizobiales ) |
title_sort |
characterization of terrihabitans soli gen. nov., sp. nov., a novel 0.2 μm-filterable soil bacterium belonging to a widely distributed lineage of hyphomicrobiales ( rhizobiales ) |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/d13090422 https://doaj.org/article/5ff9c39acc5a4d16be4661e1be2392c5 |
geographic |
Arctic Norway |
geographic_facet |
Arctic Norway |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Diversity, Vol 13, Iss 422, p 422 (2021) |
op_relation |
https://www.mdpi.com/1424-2818/13/9/422 https://doaj.org/toc/1424-2818 doi:10.3390/d13090422 1424-2818 https://doaj.org/article/5ff9c39acc5a4d16be4661e1be2392c5 |
op_doi |
https://doi.org/10.3390/d13090422 |
container_title |
Diversity |
container_volume |
13 |
container_issue |
9 |
container_start_page |
422 |
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1766340727954997248 |