The Babesia bovis hap2 gene is not required for blood stage replication, but expressed upon in vitro sexual stage induction.
Babesia bovis, is a tick borne apicomplexan parasite responsible for important cattle losses globally. Babesia parasites have a complex life cycle including asexual replication in the mammalian host and sexual reproduction in the tick vector. Novel control strategies aimed at limiting transmission o...
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ftdoajarticles:oai:doaj.org/article:5ff9877b2d8742cebc93b0a460943873 2023-05-15T15:15:16+02:00 The Babesia bovis hap2 gene is not required for blood stage replication, but expressed upon in vitro sexual stage induction. Hala E Hussein Reginaldo G Bastos David A Schneider Wendell C Johnson Fatma K Adham William C Davis Jacob M Laughery David R Herndon Heba F Alzan Massaro W Ueti Carlos E Suarez 2017-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005965 https://doaj.org/article/5ff9877b2d8742cebc93b0a460943873 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5646870?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005965 https://doaj.org/article/5ff9877b2d8742cebc93b0a460943873 PLoS Neglected Tropical Diseases, Vol 11, Iss 10, p e0005965 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005965 2022-12-31T01:36:33Z Babesia bovis, is a tick borne apicomplexan parasite responsible for important cattle losses globally. Babesia parasites have a complex life cycle including asexual replication in the mammalian host and sexual reproduction in the tick vector. Novel control strategies aimed at limiting transmission of the parasite are needed, but transmission blocking vaccine candidates remain undefined. Expression of HAP2 has been recognized as critical for the fertilization of parasites in the Babesia-related Plasmodium, and is a leading candidate for a transmission blocking vaccine against malaria. Hereby we identified the B. bovis hap2 gene and demonstrated that it is widely conserved and differentially transcribed during development within the tick midgut, but not by blood stage parasites. The hap2 gene was disrupted by transfecting B. bovis with a plasmid containing the flanking regions of the hap2 gene and the GPF-BSD gene under the control of the ef-1α-B promoter. Comparison of in vitro growth between a hap2-KO B. bovis clonal line and its parental wild type strain showed that HAP2 is not required for the development of B. bovis in erythrocytes. However, xanthurenic acid-in vitro induction experiments of sexual stages of parasites recovered after tick transmission resulted in surface expression of HAP2 exclusively in sexual stage induced parasites. In addition, hap2-KO parasites were not able to develop such sexual stages as defined both by morphology and by expression of the B. bovis sexual marker genes 6-Cys A and B. Together, the data strongly suggests that tick midgut stage differential expression of hap2 is associated with the development of B. bovis sexual forms. Overall these studies are consistent with a role of HAP2 in tick stages of the parasite and suggest that HAP2 is a potential candidate for a transmission blocking vaccine against bovine babesiosis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 10 e0005965 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Hala E Hussein Reginaldo G Bastos David A Schneider Wendell C Johnson Fatma K Adham William C Davis Jacob M Laughery David R Herndon Heba F Alzan Massaro W Ueti Carlos E Suarez The Babesia bovis hap2 gene is not required for blood stage replication, but expressed upon in vitro sexual stage induction. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Babesia bovis, is a tick borne apicomplexan parasite responsible for important cattle losses globally. Babesia parasites have a complex life cycle including asexual replication in the mammalian host and sexual reproduction in the tick vector. Novel control strategies aimed at limiting transmission of the parasite are needed, but transmission blocking vaccine candidates remain undefined. Expression of HAP2 has been recognized as critical for the fertilization of parasites in the Babesia-related Plasmodium, and is a leading candidate for a transmission blocking vaccine against malaria. Hereby we identified the B. bovis hap2 gene and demonstrated that it is widely conserved and differentially transcribed during development within the tick midgut, but not by blood stage parasites. The hap2 gene was disrupted by transfecting B. bovis with a plasmid containing the flanking regions of the hap2 gene and the GPF-BSD gene under the control of the ef-1α-B promoter. Comparison of in vitro growth between a hap2-KO B. bovis clonal line and its parental wild type strain showed that HAP2 is not required for the development of B. bovis in erythrocytes. However, xanthurenic acid-in vitro induction experiments of sexual stages of parasites recovered after tick transmission resulted in surface expression of HAP2 exclusively in sexual stage induced parasites. In addition, hap2-KO parasites were not able to develop such sexual stages as defined both by morphology and by expression of the B. bovis sexual marker genes 6-Cys A and B. Together, the data strongly suggests that tick midgut stage differential expression of hap2 is associated with the development of B. bovis sexual forms. Overall these studies are consistent with a role of HAP2 in tick stages of the parasite and suggest that HAP2 is a potential candidate for a transmission blocking vaccine against bovine babesiosis. |
format |
Article in Journal/Newspaper |
author |
Hala E Hussein Reginaldo G Bastos David A Schneider Wendell C Johnson Fatma K Adham William C Davis Jacob M Laughery David R Herndon Heba F Alzan Massaro W Ueti Carlos E Suarez |
author_facet |
Hala E Hussein Reginaldo G Bastos David A Schneider Wendell C Johnson Fatma K Adham William C Davis Jacob M Laughery David R Herndon Heba F Alzan Massaro W Ueti Carlos E Suarez |
author_sort |
Hala E Hussein |
title |
The Babesia bovis hap2 gene is not required for blood stage replication, but expressed upon in vitro sexual stage induction. |
title_short |
The Babesia bovis hap2 gene is not required for blood stage replication, but expressed upon in vitro sexual stage induction. |
title_full |
The Babesia bovis hap2 gene is not required for blood stage replication, but expressed upon in vitro sexual stage induction. |
title_fullStr |
The Babesia bovis hap2 gene is not required for blood stage replication, but expressed upon in vitro sexual stage induction. |
title_full_unstemmed |
The Babesia bovis hap2 gene is not required for blood stage replication, but expressed upon in vitro sexual stage induction. |
title_sort |
babesia bovis hap2 gene is not required for blood stage replication, but expressed upon in vitro sexual stage induction. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005965 https://doaj.org/article/5ff9877b2d8742cebc93b0a460943873 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 10, p e0005965 (2017) |
op_relation |
http://europepmc.org/articles/PMC5646870?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005965 https://doaj.org/article/5ff9877b2d8742cebc93b0a460943873 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005965 |
container_title |
PLOS Neglected Tropical Diseases |
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11 |
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10 |
container_start_page |
e0005965 |
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1766345627839496192 |