Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter
The winter Arctic Ocean is one of the most unexplored marine environments from a microbiological perspective. Heterotrophic bacteria maintain their activity at a baseline level during the extremely low energy conditions of the winter, but little is known about the specific phylotypes that have the p...
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ftdoajarticles:oai:doaj.org/article:5fd7eb40967f4dc5968ab56d6eeb4a70 2023-05-15T14:34:15+02:00 Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter Pierre E Galand Laura eAlonso-Saez Stefan eBertilsson Connie eLovejoy Emilio O. Casamayor 2013-05-01T00:00:00Z https://doi.org/10.3389/fmicb.2013.00118 https://doaj.org/article/5fd7eb40967f4dc5968ab56d6eeb4a70 EN eng Frontiers Media S.A. http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00118/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2013.00118 https://doaj.org/article/5fd7eb40967f4dc5968ab56d6eeb4a70 Frontiers in Microbiology, Vol 4 (2013) Bacteria ecotype activity BrdU Colwellia Arctic Ocean Microbiology QR1-502 article 2013 ftdoajarticles https://doi.org/10.3389/fmicb.2013.00118 2022-12-31T02:16:26Z The winter Arctic Ocean is one of the most unexplored marine environments from a microbiological perspective. Heterotrophic bacteria maintain their activity at a baseline level during the extremely low energy conditions of the winter, but little is known about the specific phylotypes that have the potential to survive and grow in such harsh environment. In this study, we aimed at identifying actively growing ribotypes in winter Arctic Ocean seawater cultures by experimental incubations with the thymidine analogue bromodeoxyuridine (BrdU), followed by immunocapturing, terminal restriction fragment length polymorphism (TRFLP) fingerprinting, cloning and sequencing the 16S rRNA gene. We incubated water collected at different months over the Arctic winter and showed that the actively growing bacterial fraction, taking up BrdU, represented only a subset of the total community. Among the BrdU-labeled bacterial taxa we identified the Flavobacteria Polaribacter, the Alphaproteobacteria SAR11, the Gammaproteobacteria Arctic 96B-16 cluster and, predominately, members of Colwellia spp. Interestingly, Colwellia sequences formed three clusters (93 and 97% pairwise 16S rRNA identity) that contributed in contrasting ways to the active communities in the incubations. Polaribacter, Arctic 96B-16 and one cluster of Colwellia were more abundant in the active community represented by the BrdU labelled DNA. In contrast, SAR11 and two other Colwellia clusters were underrepresented in the BrdU labelled community compared to total communities. Despite the limitation of the long incubations needed to label slow growing arctic communities, the BrdU approach revealed the potential for active growth in low-energy conditions in some relevant groups of polar bacteria, including Polaribacter and Arctic 96B-16. Moreover, under similar incubation conditions, the growth of different Colwellia ribotypes varied, suggesting that related clusters of Colwellia may have distinct metabolic features. Article in Journal/Newspaper Arctic Arctic Ocean Directory of Open Access Journals: DOAJ Articles Arctic Arctic Ocean Frontiers in Microbiology 4 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Bacteria ecotype activity BrdU Colwellia Arctic Ocean Microbiology QR1-502 |
spellingShingle |
Bacteria ecotype activity BrdU Colwellia Arctic Ocean Microbiology QR1-502 Pierre E Galand Laura eAlonso-Saez Stefan eBertilsson Connie eLovejoy Emilio O. Casamayor Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter |
topic_facet |
Bacteria ecotype activity BrdU Colwellia Arctic Ocean Microbiology QR1-502 |
description |
The winter Arctic Ocean is one of the most unexplored marine environments from a microbiological perspective. Heterotrophic bacteria maintain their activity at a baseline level during the extremely low energy conditions of the winter, but little is known about the specific phylotypes that have the potential to survive and grow in such harsh environment. In this study, we aimed at identifying actively growing ribotypes in winter Arctic Ocean seawater cultures by experimental incubations with the thymidine analogue bromodeoxyuridine (BrdU), followed by immunocapturing, terminal restriction fragment length polymorphism (TRFLP) fingerprinting, cloning and sequencing the 16S rRNA gene. We incubated water collected at different months over the Arctic winter and showed that the actively growing bacterial fraction, taking up BrdU, represented only a subset of the total community. Among the BrdU-labeled bacterial taxa we identified the Flavobacteria Polaribacter, the Alphaproteobacteria SAR11, the Gammaproteobacteria Arctic 96B-16 cluster and, predominately, members of Colwellia spp. Interestingly, Colwellia sequences formed three clusters (93 and 97% pairwise 16S rRNA identity) that contributed in contrasting ways to the active communities in the incubations. Polaribacter, Arctic 96B-16 and one cluster of Colwellia were more abundant in the active community represented by the BrdU labelled DNA. In contrast, SAR11 and two other Colwellia clusters were underrepresented in the BrdU labelled community compared to total communities. Despite the limitation of the long incubations needed to label slow growing arctic communities, the BrdU approach revealed the potential for active growth in low-energy conditions in some relevant groups of polar bacteria, including Polaribacter and Arctic 96B-16. Moreover, under similar incubation conditions, the growth of different Colwellia ribotypes varied, suggesting that related clusters of Colwellia may have distinct metabolic features. |
format |
Article in Journal/Newspaper |
author |
Pierre E Galand Laura eAlonso-Saez Stefan eBertilsson Connie eLovejoy Emilio O. Casamayor |
author_facet |
Pierre E Galand Laura eAlonso-Saez Stefan eBertilsson Connie eLovejoy Emilio O. Casamayor |
author_sort |
Pierre E Galand |
title |
Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter |
title_short |
Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter |
title_full |
Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter |
title_fullStr |
Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter |
title_full_unstemmed |
Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter |
title_sort |
contrasting activity patterns determined by brdu incorporation in bacterial ribotypes from the arctic ocean in winter |
publisher |
Frontiers Media S.A. |
publishDate |
2013 |
url |
https://doi.org/10.3389/fmicb.2013.00118 https://doaj.org/article/5fd7eb40967f4dc5968ab56d6eeb4a70 |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Ocean |
genre_facet |
Arctic Arctic Ocean |
op_source |
Frontiers in Microbiology, Vol 4 (2013) |
op_relation |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00118/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2013.00118 https://doaj.org/article/5fd7eb40967f4dc5968ab56d6eeb4a70 |
op_doi |
https://doi.org/10.3389/fmicb.2013.00118 |
container_title |
Frontiers in Microbiology |
container_volume |
4 |
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1766307329805910016 |