Preliminary antigenic characterisation of an adult worm vomit preparation of Fasciola hepatica by infected human sera Caracterização antigênica preliminar de preparação de vômito de verme adulto de Fasciola hepatica por soros humanos infectados
Fascioliasis is an emerging/re-emerging vector-borne disease with the widest known distribution. Approximately 17 million people are infected around the world, being the Andean region the most affected area. There is an important necessity to develop sensitive and specific diagnostic tools to treat...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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Universidade de São Paulo (USP)
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ftdoajarticles:oai:doaj.org/article:5f9b7b87374440679a3c97693a36f531 2024-09-09T19:28:26+00:00 Preliminary antigenic characterisation of an adult worm vomit preparation of Fasciola hepatica by infected human sera Caracterização antigênica preliminar de preparação de vômito de verme adulto de Fasciola hepatica por soros humanos infectados María Alejandra De Almeida María Benardetti Ferreira Sandra Planchart Angélica Terashima Vicente Maco Luis Marcos Eduardo Gotuzzo Elizabeth Sánchez César Náquira José Vicente Scorza Renzo Nino Incani 2007-02-01T00:00:00Z https://doi.org/10.1590/S0036-46652007000100006 https://doaj.org/article/5f9b7b87374440679a3c97693a36f531 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652007000100006 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652007000100006 0036-4665 1678-9946 https://doaj.org/article/5f9b7b87374440679a3c97693a36f531 Revista do Instituto de Medicina Tropical de São Paulo, Vol 49, Iss 1, Pp 31-35 (2007) Fasciola hepatica Western blot Worm vomit antigens Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2007 ftdoajarticles https://doi.org/10.1590/S0036-46652007000100006 2024-08-05T17:49:31Z Fascioliasis is an emerging/re-emerging vector-borne disease with the widest known distribution. Approximately 17 million people are infected around the world, being the Andean region the most affected area. There is an important necessity to develop sensitive and specific diagnostic tools to treat patients early and to avoid complications. In this paper we evaluated the immune response of infected humans against two antigenic preparations: the total soluble extract (FhTSE) and the adult worm vomit (FhAWV) in order to identify antigenic fractions specific for Fasciola hepatica. Both preparations were processed by SDS-PAGE and Western blot with human sera with fascioliasis (F), other parasitosis and healthy individuals. In the immunoblot of FhTSE, sera F recognised 16 bands with MW between eight and 110 kDa, from which those of 8, 9, 10, 38, 45 and 57 kDa were specific. In the preparation FhAWV, sera F recognised nine bands with MW from eight to 85 kDa, from which those of 8, 12, 15 and 24 kDa were specific. Some bands of cross-reaction were evident with sera from patients with other parasitoses, more frequent with the FhTSE. Bands within the MW mentioned, particularly that of eight kDa, have been shown to be specific by others, and deserve additional characterisation for their potential use in immunodiagnosis. Fasciolíase é uma doença emergente/re-emergente transmitida por vetores com a distribuição sabidamente mais ampla. Existem aproximadamente 17 milhões de pessoas infectadas em todo mundo, sendo a região andina a área mais afetada. Há uma necessidade importante para desenvolver ferramentas diagnósticas sensíveis e específicas para tratar cedo os pacientes e para evitar complicações. Neste trabalho avaliamos a resposta imune de seres humanos infectados comparando a duas preparações antigênicas: o extrato solúvel total (FhTSE) e o vômito (FhAWV) do verme adulto a fim de identificar as frações antigênicas específicas para Fasciola hepatica. Ambas as preparações foram processadas por SDS-PAGE e Western blot com ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 49 1 31 35 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Fasciola hepatica Western blot Worm vomit antigens Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Fasciola hepatica Western blot Worm vomit antigens Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 María Alejandra De Almeida María Benardetti Ferreira Sandra Planchart Angélica Terashima Vicente Maco Luis Marcos Eduardo Gotuzzo Elizabeth Sánchez César Náquira José Vicente Scorza Renzo Nino Incani Preliminary antigenic characterisation of an adult worm vomit preparation of Fasciola hepatica by infected human sera Caracterização antigênica preliminar de preparação de vômito de verme adulto de Fasciola hepatica por soros humanos infectados |
topic_facet |
Fasciola hepatica Western blot Worm vomit antigens Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Fascioliasis is an emerging/re-emerging vector-borne disease with the widest known distribution. Approximately 17 million people are infected around the world, being the Andean region the most affected area. There is an important necessity to develop sensitive and specific diagnostic tools to treat patients early and to avoid complications. In this paper we evaluated the immune response of infected humans against two antigenic preparations: the total soluble extract (FhTSE) and the adult worm vomit (FhAWV) in order to identify antigenic fractions specific for Fasciola hepatica. Both preparations were processed by SDS-PAGE and Western blot with human sera with fascioliasis (F), other parasitosis and healthy individuals. In the immunoblot of FhTSE, sera F recognised 16 bands with MW between eight and 110 kDa, from which those of 8, 9, 10, 38, 45 and 57 kDa were specific. In the preparation FhAWV, sera F recognised nine bands with MW from eight to 85 kDa, from which those of 8, 12, 15 and 24 kDa were specific. Some bands of cross-reaction were evident with sera from patients with other parasitoses, more frequent with the FhTSE. Bands within the MW mentioned, particularly that of eight kDa, have been shown to be specific by others, and deserve additional characterisation for their potential use in immunodiagnosis. Fasciolíase é uma doença emergente/re-emergente transmitida por vetores com a distribuição sabidamente mais ampla. Existem aproximadamente 17 milhões de pessoas infectadas em todo mundo, sendo a região andina a área mais afetada. Há uma necessidade importante para desenvolver ferramentas diagnósticas sensíveis e específicas para tratar cedo os pacientes e para evitar complicações. Neste trabalho avaliamos a resposta imune de seres humanos infectados comparando a duas preparações antigênicas: o extrato solúvel total (FhTSE) e o vômito (FhAWV) do verme adulto a fim de identificar as frações antigênicas específicas para Fasciola hepatica. Ambas as preparações foram processadas por SDS-PAGE e Western blot com ... |
format |
Article in Journal/Newspaper |
author |
María Alejandra De Almeida María Benardetti Ferreira Sandra Planchart Angélica Terashima Vicente Maco Luis Marcos Eduardo Gotuzzo Elizabeth Sánchez César Náquira José Vicente Scorza Renzo Nino Incani |
author_facet |
María Alejandra De Almeida María Benardetti Ferreira Sandra Planchart Angélica Terashima Vicente Maco Luis Marcos Eduardo Gotuzzo Elizabeth Sánchez César Náquira José Vicente Scorza Renzo Nino Incani |
author_sort |
María Alejandra De Almeida |
title |
Preliminary antigenic characterisation of an adult worm vomit preparation of Fasciola hepatica by infected human sera Caracterização antigênica preliminar de preparação de vômito de verme adulto de Fasciola hepatica por soros humanos infectados |
title_short |
Preliminary antigenic characterisation of an adult worm vomit preparation of Fasciola hepatica by infected human sera Caracterização antigênica preliminar de preparação de vômito de verme adulto de Fasciola hepatica por soros humanos infectados |
title_full |
Preliminary antigenic characterisation of an adult worm vomit preparation of Fasciola hepatica by infected human sera Caracterização antigênica preliminar de preparação de vômito de verme adulto de Fasciola hepatica por soros humanos infectados |
title_fullStr |
Preliminary antigenic characterisation of an adult worm vomit preparation of Fasciola hepatica by infected human sera Caracterização antigênica preliminar de preparação de vômito de verme adulto de Fasciola hepatica por soros humanos infectados |
title_full_unstemmed |
Preliminary antigenic characterisation of an adult worm vomit preparation of Fasciola hepatica by infected human sera Caracterização antigênica preliminar de preparação de vômito de verme adulto de Fasciola hepatica por soros humanos infectados |
title_sort |
preliminary antigenic characterisation of an adult worm vomit preparation of fasciola hepatica by infected human sera caracterização antigênica preliminar de preparação de vômito de verme adulto de fasciola hepatica por soros humanos infectados |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2007 |
url |
https://doi.org/10.1590/S0036-46652007000100006 https://doaj.org/article/5f9b7b87374440679a3c97693a36f531 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 49, Iss 1, Pp 31-35 (2007) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652007000100006 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652007000100006 0036-4665 1678-9946 https://doaj.org/article/5f9b7b87374440679a3c97693a36f531 |
op_doi |
https://doi.org/10.1590/S0036-46652007000100006 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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49 |
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