Dengue 1 diversity and microevolution, French Polynesia 2001-2006: connection with epidemiology and clinics.
BACKGROUND: Dengue fever (DF) is an emerging infectious disease in the tropics and subtropics. Determinants of DF epidemiology and factors involved in severe cases-dengue haemorrhagic fever (DHF) and dengue shock syndrome (DSS)-remain imperfectly characterized. Since 2000, serotype 1 (DENV-1) has pr...
Published in: | PLoS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2009
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0000493 https://doaj.org/article/5f8c9e554ccd471496e52b04d9c11584 |
id |
ftdoajarticles:oai:doaj.org/article:5f8c9e554ccd471496e52b04d9c11584 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:5f8c9e554ccd471496e52b04d9c11584 2023-05-15T15:14:32+02:00 Dengue 1 diversity and microevolution, French Polynesia 2001-2006: connection with epidemiology and clinics. Elodie Descloux Van-Mai Cao-Lormeau Claudine Roche Xavier De Lamballerie 2009-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000493 https://doaj.org/article/5f8c9e554ccd471496e52b04d9c11584 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2714178?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000493 https://doaj.org/article/5f8c9e554ccd471496e52b04d9c11584 PLoS Neglected Tropical Diseases, Vol 3, Iss 8, p e493 (2009) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2009 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000493 2022-12-31T01:08:15Z BACKGROUND: Dengue fever (DF) is an emerging infectious disease in the tropics and subtropics. Determinants of DF epidemiology and factors involved in severe cases-dengue haemorrhagic fever (DHF) and dengue shock syndrome (DSS)-remain imperfectly characterized. Since 2000, serotype 1 (DENV-1) has predominated in the South Pacific. The aim of this study was (i) to determine the origin and (ii) to study the evolutionary relationships of DENV-1 viruses that have circulated in French Polynesia (FP) from the severe 2001 outbreak to the recent 2006 epidemic, and (iii) to analyse the viral intra-host genetic diversity according to clinical presentation. METHODOLOGY/PRINCIPAL FINDINGS: Sequences of 181 envelope gene and 12 complete polyproteins of DENV-1 viruses obtained from human sera in FP during the 2001-2006 period were generated. Phylogenetic analysis showed that all DENV-1 FP strains belonged to genotype IV-"South Pacific" and derived from a single introduction event from South-East Asia followed by a 6-year in situ evolution. Although the ratio of nonsynonymous/synonymous substitutions per site indicated strong negative selection, a mutation in the envelope glycoprotein (S222T) appeared in 2002 and was subsequently fixed. It was noted that genetic diversification was very significant during the 2002-2005 period of endemic DENV-1 circulation. For nine DF sera and eight DHF/DSS sera, approximately 40 clones/serum of partial envelope gene were sequenced. Importantly, analysis revealed that the intra-host genetic diversity was significantly lower in severe cases than in classical DF. CONCLUSIONS/SIGNIFICANCE: First, this study showed that DENV-1 epidemiology in FP was different from that described in other South-Pacific islands, characterized by a long sustained viral circulation and the absence of new viral introduction over a 6-year period. Second, a significant part of DENV-1 evolution was observed during the endemic period characterized by the rapid fixation of S222T in the envelope protein that may reflect ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Pacific PLoS Neglected Tropical Diseases 3 8 e493 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Elodie Descloux Van-Mai Cao-Lormeau Claudine Roche Xavier De Lamballerie Dengue 1 diversity and microevolution, French Polynesia 2001-2006: connection with epidemiology and clinics. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Dengue fever (DF) is an emerging infectious disease in the tropics and subtropics. Determinants of DF epidemiology and factors involved in severe cases-dengue haemorrhagic fever (DHF) and dengue shock syndrome (DSS)-remain imperfectly characterized. Since 2000, serotype 1 (DENV-1) has predominated in the South Pacific. The aim of this study was (i) to determine the origin and (ii) to study the evolutionary relationships of DENV-1 viruses that have circulated in French Polynesia (FP) from the severe 2001 outbreak to the recent 2006 epidemic, and (iii) to analyse the viral intra-host genetic diversity according to clinical presentation. METHODOLOGY/PRINCIPAL FINDINGS: Sequences of 181 envelope gene and 12 complete polyproteins of DENV-1 viruses obtained from human sera in FP during the 2001-2006 period were generated. Phylogenetic analysis showed that all DENV-1 FP strains belonged to genotype IV-"South Pacific" and derived from a single introduction event from South-East Asia followed by a 6-year in situ evolution. Although the ratio of nonsynonymous/synonymous substitutions per site indicated strong negative selection, a mutation in the envelope glycoprotein (S222T) appeared in 2002 and was subsequently fixed. It was noted that genetic diversification was very significant during the 2002-2005 period of endemic DENV-1 circulation. For nine DF sera and eight DHF/DSS sera, approximately 40 clones/serum of partial envelope gene were sequenced. Importantly, analysis revealed that the intra-host genetic diversity was significantly lower in severe cases than in classical DF. CONCLUSIONS/SIGNIFICANCE: First, this study showed that DENV-1 epidemiology in FP was different from that described in other South-Pacific islands, characterized by a long sustained viral circulation and the absence of new viral introduction over a 6-year period. Second, a significant part of DENV-1 evolution was observed during the endemic period characterized by the rapid fixation of S222T in the envelope protein that may reflect ... |
format |
Article in Journal/Newspaper |
author |
Elodie Descloux Van-Mai Cao-Lormeau Claudine Roche Xavier De Lamballerie |
author_facet |
Elodie Descloux Van-Mai Cao-Lormeau Claudine Roche Xavier De Lamballerie |
author_sort |
Elodie Descloux |
title |
Dengue 1 diversity and microevolution, French Polynesia 2001-2006: connection with epidemiology and clinics. |
title_short |
Dengue 1 diversity and microevolution, French Polynesia 2001-2006: connection with epidemiology and clinics. |
title_full |
Dengue 1 diversity and microevolution, French Polynesia 2001-2006: connection with epidemiology and clinics. |
title_fullStr |
Dengue 1 diversity and microevolution, French Polynesia 2001-2006: connection with epidemiology and clinics. |
title_full_unstemmed |
Dengue 1 diversity and microevolution, French Polynesia 2001-2006: connection with epidemiology and clinics. |
title_sort |
dengue 1 diversity and microevolution, french polynesia 2001-2006: connection with epidemiology and clinics. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2009 |
url |
https://doi.org/10.1371/journal.pntd.0000493 https://doaj.org/article/5f8c9e554ccd471496e52b04d9c11584 |
geographic |
Arctic Pacific |
geographic_facet |
Arctic Pacific |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 3, Iss 8, p e493 (2009) |
op_relation |
http://europepmc.org/articles/PMC2714178?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000493 https://doaj.org/article/5f8c9e554ccd471496e52b04d9c11584 |
op_doi |
https://doi.org/10.1371/journal.pntd.0000493 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
3 |
container_issue |
8 |
container_start_page |
e493 |
_version_ |
1766344980695089152 |