Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria
Abstract Background Cerebral malaria (CM) is an acute encephalopathy with increased pro-inflammatory cytokines, sequestration of parasitized erythrocytes and localized ischaemia. In children CM induces cognitive impairment in about 10% of the survivors. Erythropoietin (Epo) has – besides of its well...
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ftdoajarticles:oai:doaj.org/article:5f19b95f9b974ef1ab4798e5e1e12637 2023-05-15T15:08:41+02:00 Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria Hempel Casper Wiese Lothar Penkowa Milena Kirkby Nikolai Kurtzhals Jørgen AL 2008-01-01T00:00:00Z https://doi.org/10.1186/1475-2875-7-3 https://doaj.org/article/5f19b95f9b974ef1ab4798e5e1e12637 EN eng BMC http://www.malariajournal.com/content/7/1/3 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-7-3 1475-2875 https://doaj.org/article/5f19b95f9b974ef1ab4798e5e1e12637 Malaria Journal, Vol 7, Iss 1, p 3 (2008) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2008 ftdoajarticles https://doi.org/10.1186/1475-2875-7-3 2022-12-31T08:15:57Z Abstract Background Cerebral malaria (CM) is an acute encephalopathy with increased pro-inflammatory cytokines, sequestration of parasitized erythrocytes and localized ischaemia. In children CM induces cognitive impairment in about 10% of the survivors. Erythropoietin (Epo) has – besides of its well known haematopoietic properties – significant anti-inflammatory, antioxidant and anti-apoptotic effects in various brain disorders. The neurobiological responses to exogenously injected Epo during murine CM were examined. Methods Female C57BL/6j mice (4–6 weeks), infected with Plasmodium berghei ANKA, were treated with recombinant human Epo (rhEpo; 50–5000 U/kg/OD, i.p.) at different time points. The effect on survival was measured. Brain pathology was investigated by TUNEL (Terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-digoxigenin nick end labelling), as a marker of apoptosis. Gene expression in brain tissue was measured by real time PCR. Results Treatment with rhEpo increased survival in mice with CM in a dose- and time-dependent manner and reduced apoptotic cell death of neurons as well as the expression of pro-inflammatory cytokines in the brain. This neuroprotective effect appeared to be independent of the haematopoietic effect. Conclusion These results and its excellent safety profile in humans makes rhEpo a potential candidate for adjunct treatment of CM. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 7 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Hempel Casper Wiese Lothar Penkowa Milena Kirkby Nikolai Kurtzhals Jørgen AL Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Cerebral malaria (CM) is an acute encephalopathy with increased pro-inflammatory cytokines, sequestration of parasitized erythrocytes and localized ischaemia. In children CM induces cognitive impairment in about 10% of the survivors. Erythropoietin (Epo) has – besides of its well known haematopoietic properties – significant anti-inflammatory, antioxidant and anti-apoptotic effects in various brain disorders. The neurobiological responses to exogenously injected Epo during murine CM were examined. Methods Female C57BL/6j mice (4–6 weeks), infected with Plasmodium berghei ANKA, were treated with recombinant human Epo (rhEpo; 50–5000 U/kg/OD, i.p.) at different time points. The effect on survival was measured. Brain pathology was investigated by TUNEL (Terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-digoxigenin nick end labelling), as a marker of apoptosis. Gene expression in brain tissue was measured by real time PCR. Results Treatment with rhEpo increased survival in mice with CM in a dose- and time-dependent manner and reduced apoptotic cell death of neurons as well as the expression of pro-inflammatory cytokines in the brain. This neuroprotective effect appeared to be independent of the haematopoietic effect. Conclusion These results and its excellent safety profile in humans makes rhEpo a potential candidate for adjunct treatment of CM. |
format |
Article in Journal/Newspaper |
author |
Hempel Casper Wiese Lothar Penkowa Milena Kirkby Nikolai Kurtzhals Jørgen AL |
author_facet |
Hempel Casper Wiese Lothar Penkowa Milena Kirkby Nikolai Kurtzhals Jørgen AL |
author_sort |
Hempel Casper |
title |
Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria |
title_short |
Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria |
title_full |
Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria |
title_fullStr |
Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria |
title_full_unstemmed |
Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria |
title_sort |
recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria |
publisher |
BMC |
publishDate |
2008 |
url |
https://doi.org/10.1186/1475-2875-7-3 https://doaj.org/article/5f19b95f9b974ef1ab4798e5e1e12637 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 7, Iss 1, p 3 (2008) |
op_relation |
http://www.malariajournal.com/content/7/1/3 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-7-3 1475-2875 https://doaj.org/article/5f19b95f9b974ef1ab4798e5e1e12637 |
op_doi |
https://doi.org/10.1186/1475-2875-7-3 |
container_title |
Malaria Journal |
container_volume |
7 |
container_issue |
1 |
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1766339993407586304 |