Cryo-Microtome sections of coproculture larvae of Strongyloides stercoralis and Strongyloides ratti as antigen sources for the immunodiagnosis of human strongyloidiasis
Cryo-microtome sections of larvae of Strongyloides stercoralis and S. ratti respectively obtained from human and rat feces cultures, were used as antigens. Fluoresceinate conjugates against human IgG were employed at the ideal titer of 10 for S. stercoralis and 100 for S. ratti. The sensitivity of t...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Universidade de São Paulo (USP)
1997
|
Subjects: | |
Online Access: | https://doi.org/10.1590/S0036-46651997000600001 https://doaj.org/article/5f0441fcd255400a8ad853d905303c9b |
id |
ftdoajarticles:oai:doaj.org/article:5f0441fcd255400a8ad853d905303c9b |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:5f0441fcd255400a8ad853d905303c9b 2024-09-09T19:28:17+00:00 Cryo-Microtome sections of coproculture larvae of Strongyloides stercoralis and Strongyloides ratti as antigen sources for the immunodiagnosis of human strongyloidiasis Julia Maria COSTA-CRUZ Carina Barbosa BULLAMAH Maria do Rosário F. GONÇALVES-PIRES Dulcinéa Maria B. CAMPOS Miguel Alípio VIEIRA 1997-11-01T00:00:00Z https://doi.org/10.1590/S0036-46651997000600001 https://doaj.org/article/5f0441fcd255400a8ad853d905303c9b EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651997000600001 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651997000600001 0036-4665 1678-9946 https://doaj.org/article/5f0441fcd255400a8ad853d905303c9b Revista do Instituto de Medicina Tropical de São Paulo, Vol 39, Iss 6, p 313 (1997) Strongyloides stercoralis Strongyloides ratti Indirect immunofluorescence reaction Diagnosis Human strongyloidiasis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1997 ftdoajarticles https://doi.org/10.1590/S0036-46651997000600001 2024-08-05T17:49:30Z Cryo-microtome sections of larvae of Strongyloides stercoralis and S. ratti respectively obtained from human and rat feces cultures, were used as antigens. Fluoresceinate conjugates against human IgG were employed at the ideal titer of 10 for S. stercoralis and 100 for S. ratti. The sensitivity of the indirect immunofluorescence reaction (IIF) was 94.4% and 92.5% and the specificity 94.2% and 97.1% for the two specific larval antigens, respectively. Sera from 123 persons (54 from carriers of S. stercoralis infections and 69 from controls) were submitted to the reaction. The titers of different sera varied from 20 to 2560. There was a significant linear correlation (r = 0.85 p £ 0.001) between the antibodies from the two species of larval antigens. We conclude that both antigens may be used in the IIF reaction for the diagnosis of human strongyloidiasis. Due to the feasibility of safe and low-cost mass production of S. ratti larvae in the laboratory with a considerable economy of conjugate, their utilization in the serum diagnosis of human strongyloidiasis is recommended Comparou-se o emprego de cortes em congelação de larvas de S. stercoralis e de S. ratti como fonte de antígenos na reação de imunofluorescência indireta (RIF) para o diagnóstico da estrongiloidíase humana. Os antígenos foram obtidos de coproculturas de fezes humanas e de ratos, respectivamente. Soros de 123 indivíduos foram analisados sendo 54 de pacientes com estrongiloidíase e 69 controles. Empregou-se conjugado anti IgG humano marcado com fluoresceína nos títulos ideais de 10 para S. stercoralis e 100 para S. ratti. A sensibilidade da RIF foi de 94,4% e 92,5% e a especificidade de 94,2% e 97,1% respectivamente para os antígenos de S. stercoralis e S. ratti. Para os 2 antígenos os títulos dos soros variaram de 20 a 2560. Houve correlação linear significativa (r = 0,85 p £ 0,001) entre os títulos de anticorpos para os 2 antígenos. Concluiu-se que os 2 antígenos podem ser empregados na RIF. Diante da disponibilidade de obtenção de grande ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Rif ENVELOPE(-16.172,-16.172,66.526,66.526) Revista do Instituto de Medicina Tropical de São Paulo 39 6 313 318 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Strongyloides stercoralis Strongyloides ratti Indirect immunofluorescence reaction Diagnosis Human strongyloidiasis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Strongyloides stercoralis Strongyloides ratti Indirect immunofluorescence reaction Diagnosis Human strongyloidiasis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Julia Maria COSTA-CRUZ Carina Barbosa BULLAMAH Maria do Rosário F. GONÇALVES-PIRES Dulcinéa Maria B. CAMPOS Miguel Alípio VIEIRA Cryo-Microtome sections of coproculture larvae of Strongyloides stercoralis and Strongyloides ratti as antigen sources for the immunodiagnosis of human strongyloidiasis |
topic_facet |
Strongyloides stercoralis Strongyloides ratti Indirect immunofluorescence reaction Diagnosis Human strongyloidiasis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Cryo-microtome sections of larvae of Strongyloides stercoralis and S. ratti respectively obtained from human and rat feces cultures, were used as antigens. Fluoresceinate conjugates against human IgG were employed at the ideal titer of 10 for S. stercoralis and 100 for S. ratti. The sensitivity of the indirect immunofluorescence reaction (IIF) was 94.4% and 92.5% and the specificity 94.2% and 97.1% for the two specific larval antigens, respectively. Sera from 123 persons (54 from carriers of S. stercoralis infections and 69 from controls) were submitted to the reaction. The titers of different sera varied from 20 to 2560. There was a significant linear correlation (r = 0.85 p £ 0.001) between the antibodies from the two species of larval antigens. We conclude that both antigens may be used in the IIF reaction for the diagnosis of human strongyloidiasis. Due to the feasibility of safe and low-cost mass production of S. ratti larvae in the laboratory with a considerable economy of conjugate, their utilization in the serum diagnosis of human strongyloidiasis is recommended Comparou-se o emprego de cortes em congelação de larvas de S. stercoralis e de S. ratti como fonte de antígenos na reação de imunofluorescência indireta (RIF) para o diagnóstico da estrongiloidíase humana. Os antígenos foram obtidos de coproculturas de fezes humanas e de ratos, respectivamente. Soros de 123 indivíduos foram analisados sendo 54 de pacientes com estrongiloidíase e 69 controles. Empregou-se conjugado anti IgG humano marcado com fluoresceína nos títulos ideais de 10 para S. stercoralis e 100 para S. ratti. A sensibilidade da RIF foi de 94,4% e 92,5% e a especificidade de 94,2% e 97,1% respectivamente para os antígenos de S. stercoralis e S. ratti. Para os 2 antígenos os títulos dos soros variaram de 20 a 2560. Houve correlação linear significativa (r = 0,85 p £ 0,001) entre os títulos de anticorpos para os 2 antígenos. Concluiu-se que os 2 antígenos podem ser empregados na RIF. Diante da disponibilidade de obtenção de grande ... |
format |
Article in Journal/Newspaper |
author |
Julia Maria COSTA-CRUZ Carina Barbosa BULLAMAH Maria do Rosário F. GONÇALVES-PIRES Dulcinéa Maria B. CAMPOS Miguel Alípio VIEIRA |
author_facet |
Julia Maria COSTA-CRUZ Carina Barbosa BULLAMAH Maria do Rosário F. GONÇALVES-PIRES Dulcinéa Maria B. CAMPOS Miguel Alípio VIEIRA |
author_sort |
Julia Maria COSTA-CRUZ |
title |
Cryo-Microtome sections of coproculture larvae of Strongyloides stercoralis and Strongyloides ratti as antigen sources for the immunodiagnosis of human strongyloidiasis |
title_short |
Cryo-Microtome sections of coproculture larvae of Strongyloides stercoralis and Strongyloides ratti as antigen sources for the immunodiagnosis of human strongyloidiasis |
title_full |
Cryo-Microtome sections of coproculture larvae of Strongyloides stercoralis and Strongyloides ratti as antigen sources for the immunodiagnosis of human strongyloidiasis |
title_fullStr |
Cryo-Microtome sections of coproculture larvae of Strongyloides stercoralis and Strongyloides ratti as antigen sources for the immunodiagnosis of human strongyloidiasis |
title_full_unstemmed |
Cryo-Microtome sections of coproculture larvae of Strongyloides stercoralis and Strongyloides ratti as antigen sources for the immunodiagnosis of human strongyloidiasis |
title_sort |
cryo-microtome sections of coproculture larvae of strongyloides stercoralis and strongyloides ratti as antigen sources for the immunodiagnosis of human strongyloidiasis |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1997 |
url |
https://doi.org/10.1590/S0036-46651997000600001 https://doaj.org/article/5f0441fcd255400a8ad853d905303c9b |
long_lat |
ENVELOPE(-16.172,-16.172,66.526,66.526) |
geographic |
Arctic Rif |
geographic_facet |
Arctic Rif |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 39, Iss 6, p 313 (1997) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651997000600001 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651997000600001 0036-4665 1678-9946 https://doaj.org/article/5f0441fcd255400a8ad853d905303c9b |
op_doi |
https://doi.org/10.1590/S0036-46651997000600001 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
39 |
container_issue |
6 |
container_start_page |
313 |
op_container_end_page |
318 |
_version_ |
1809897553530978304 |