Expression of intra- and extracellular granzymes in patients with typhoid fever.
Typhoid fever, caused by the intracellular pathogen Salmonella (S.) enterica serovar Typhi, remains a major cause of morbidity and mortality worldwide. Granzymes are serine proteases promoting cytotoxic lymphocytes mediated eradication of intracellular pathogens via the induction of cell death and w...
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ftdoajarticles:oai:doaj.org/article:5ef6b617943a479385f0c841baff6a18 2023-05-15T15:13:51+02:00 Expression of intra- and extracellular granzymes in patients with typhoid fever. Hanna K de Jong Maria Isabel Garcia-Laorden Arie J Hoogendijk Christopher M Parry Rapeephan R Maude Arjen M Dondorp Mohammed Abul Faiz Tom van der Poll Willem Joost Wiersinga 2017-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005823 https://doaj.org/article/5ef6b617943a479385f0c841baff6a18 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5549753?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005823 https://doaj.org/article/5ef6b617943a479385f0c841baff6a18 PLoS Neglected Tropical Diseases, Vol 11, Iss 7, p e0005823 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005823 2022-12-30T23:25:28Z Typhoid fever, caused by the intracellular pathogen Salmonella (S.) enterica serovar Typhi, remains a major cause of morbidity and mortality worldwide. Granzymes are serine proteases promoting cytotoxic lymphocytes mediated eradication of intracellular pathogens via the induction of cell death and which can also play a role in inflammation. We aimed to characterize the expression of extracellular and intracellular granzymes in patients with typhoid fever and whether the extracellular levels of granzyme correlated with IFN-γ release.We analyzed soluble protein levels of extracellular granzyme A and B in healthy volunteers and patients with confirmed S. Typhi infection on admission and day of discharge, and investigated whether this correlated with interferon (IFN)-γ release, a cytokine significantly expressed in typhoid fever. The intracellular expression of granzyme A, B and K in subsets of lymphocytic cells was determined using flow cytometry. Patients demonstrated a marked increase of extracellular granzyme A and B in acute phase plasma and a correlation of both granzymes with IFN-γ release. In patients, lower plasma levels of granzyme B, but not granzyme A, were found at day of discharge compared to admission, indicating an association of granzyme B with stage of disease. Peripheral blood mononuclear cells of typhoid fever patients had a higher percentage of lymphocytic cells expressing intracellular granzyme A and granzyme B, but not granzyme K, compared to controls.The marked increase observed in extra- and intracellular levels of granzyme expression in patients with typhoid fever, and the correlation with stage of disease, suggests a role for granzymes in the host response to this disease. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 7 e0005823 |
institution |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Hanna K de Jong Maria Isabel Garcia-Laorden Arie J Hoogendijk Christopher M Parry Rapeephan R Maude Arjen M Dondorp Mohammed Abul Faiz Tom van der Poll Willem Joost Wiersinga Expression of intra- and extracellular granzymes in patients with typhoid fever. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Typhoid fever, caused by the intracellular pathogen Salmonella (S.) enterica serovar Typhi, remains a major cause of morbidity and mortality worldwide. Granzymes are serine proteases promoting cytotoxic lymphocytes mediated eradication of intracellular pathogens via the induction of cell death and which can also play a role in inflammation. We aimed to characterize the expression of extracellular and intracellular granzymes in patients with typhoid fever and whether the extracellular levels of granzyme correlated with IFN-γ release.We analyzed soluble protein levels of extracellular granzyme A and B in healthy volunteers and patients with confirmed S. Typhi infection on admission and day of discharge, and investigated whether this correlated with interferon (IFN)-γ release, a cytokine significantly expressed in typhoid fever. The intracellular expression of granzyme A, B and K in subsets of lymphocytic cells was determined using flow cytometry. Patients demonstrated a marked increase of extracellular granzyme A and B in acute phase plasma and a correlation of both granzymes with IFN-γ release. In patients, lower plasma levels of granzyme B, but not granzyme A, were found at day of discharge compared to admission, indicating an association of granzyme B with stage of disease. Peripheral blood mononuclear cells of typhoid fever patients had a higher percentage of lymphocytic cells expressing intracellular granzyme A and granzyme B, but not granzyme K, compared to controls.The marked increase observed in extra- and intracellular levels of granzyme expression in patients with typhoid fever, and the correlation with stage of disease, suggests a role for granzymes in the host response to this disease. |
format |
Article in Journal/Newspaper |
author |
Hanna K de Jong Maria Isabel Garcia-Laorden Arie J Hoogendijk Christopher M Parry Rapeephan R Maude Arjen M Dondorp Mohammed Abul Faiz Tom van der Poll Willem Joost Wiersinga |
author_facet |
Hanna K de Jong Maria Isabel Garcia-Laorden Arie J Hoogendijk Christopher M Parry Rapeephan R Maude Arjen M Dondorp Mohammed Abul Faiz Tom van der Poll Willem Joost Wiersinga |
author_sort |
Hanna K de Jong |
title |
Expression of intra- and extracellular granzymes in patients with typhoid fever. |
title_short |
Expression of intra- and extracellular granzymes in patients with typhoid fever. |
title_full |
Expression of intra- and extracellular granzymes in patients with typhoid fever. |
title_fullStr |
Expression of intra- and extracellular granzymes in patients with typhoid fever. |
title_full_unstemmed |
Expression of intra- and extracellular granzymes in patients with typhoid fever. |
title_sort |
expression of intra- and extracellular granzymes in patients with typhoid fever. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005823 https://doaj.org/article/5ef6b617943a479385f0c841baff6a18 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 7, p e0005823 (2017) |
op_relation |
http://europepmc.org/articles/PMC5549753?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005823 https://doaj.org/article/5ef6b617943a479385f0c841baff6a18 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005823 |
container_title |
PLOS Neglected Tropical Diseases |
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11 |
container_issue |
7 |
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e0005823 |
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