Status of insecticide resistance and its biochemical and molecular mechanisms in Anopheles stephensi (Diptera: Culicidae) from Afghanistan
Abstract Background Insecticide resistance of Anopheles stephensi, the main malaria vector in eastern Afghanistan, has been reported previously. This study describes the biochemical and molecular mechanisms of resistance to facilitate effective vector control and insecticide resistance management. M...
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ftdoajarticles:oai:doaj.org/article:5edbd6f3a55a4eb690b0aab397a83830 2023-05-15T15:16:39+02:00 Status of insecticide resistance and its biochemical and molecular mechanisms in Anopheles stephensi (Diptera: Culicidae) from Afghanistan Noor Halim Zahid Safi Abdul Ali Ahmadi Sami Nahzat Supriya Warusavithana Naimullah Safi Reza Valadan Atie Shemshadian Marzieh Sharifi Ahmadali Enayati Janet Hemingway 2019-07-01T00:00:00Z https://doi.org/10.1186/s12936-019-2884-x https://doaj.org/article/5edbd6f3a55a4eb690b0aab397a83830 EN eng BMC http://link.springer.com/article/10.1186/s12936-019-2884-x https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2884-x 1475-2875 https://doaj.org/article/5edbd6f3a55a4eb690b0aab397a83830 Malaria Journal, Vol 18, Iss 1, Pp 1-12 (2019) Anopheles stephensi Insecticide resistance Metabolism kdr Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2019 ftdoajarticles https://doi.org/10.1186/s12936-019-2884-x 2022-12-31T11:30:25Z Abstract Background Insecticide resistance of Anopheles stephensi, the main malaria vector in eastern Afghanistan, has been reported previously. This study describes the biochemical and molecular mechanisms of resistance to facilitate effective vector control and insecticide resistance management. Methods Mosquito larvae were collected from the provinces of Kunar, Laghman and Nangarhar from 2014 to 2017. The susceptibility of the reared 3–4 days old adults was tested with deltamethrin 0.05%, bendiocarb 0.1%, malathion 5%, permethrin 0.75% and DDT 4%. Cytochrome P450 content and general esterase, glutathione S-transferase (GST) and acetylcholinesterase (AChE) activities were measured in the three field populations and the results were compared with those of the laboratory susceptible An. stephensi Beech strain. Two separate allele-specific PCR assays were used to identify L1014, L1014F and L1014S mutations in the voltage gated sodium channel gene of An. stephensi. Probit analysis, ANOVA and Hardy–Weinberg equilibrium were used to analyse bioassay, biochemical assay and gene frequency data respectively. Results The population of An. stephensi from Kunar was susceptible to bendiocarb, apart from this, all populations were resistant to all the other insecticides tested. The differences between all values for cytochrome P450s, general esterases, GSTs and AChE inhibition rates in the Kunar, Laghman and Nangarhar populations were statistically significant when compared to the Beech strain, excluding GST activities between Kunar and Beech due to the high standard deviation in Kunar. The three different sodium channel alleles [L1014 (wild type), L1014F (kdr west) and L1014S (kdr east)] were all segregated in the Afghan populations. The frequencies of kdr east mutation were 22.9%, 32.7% and 35% in Kunar, Laghman and Nangarhar populations respectively. Kdr west was at the lowest frequency of 4.44%. Conclusions Resistance to different groups of insecticides in the field populations of An. stephensi from Kunar, Laghman and ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Kunar ENVELOPE(104.296,104.296,77.666,77.666) Malaria Journal 18 1 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Anopheles stephensi Insecticide resistance Metabolism kdr Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Anopheles stephensi Insecticide resistance Metabolism kdr Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Noor Halim Zahid Safi Abdul Ali Ahmadi Sami Nahzat Supriya Warusavithana Naimullah Safi Reza Valadan Atie Shemshadian Marzieh Sharifi Ahmadali Enayati Janet Hemingway Status of insecticide resistance and its biochemical and molecular mechanisms in Anopheles stephensi (Diptera: Culicidae) from Afghanistan |
topic_facet |
Anopheles stephensi Insecticide resistance Metabolism kdr Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Insecticide resistance of Anopheles stephensi, the main malaria vector in eastern Afghanistan, has been reported previously. This study describes the biochemical and molecular mechanisms of resistance to facilitate effective vector control and insecticide resistance management. Methods Mosquito larvae were collected from the provinces of Kunar, Laghman and Nangarhar from 2014 to 2017. The susceptibility of the reared 3–4 days old adults was tested with deltamethrin 0.05%, bendiocarb 0.1%, malathion 5%, permethrin 0.75% and DDT 4%. Cytochrome P450 content and general esterase, glutathione S-transferase (GST) and acetylcholinesterase (AChE) activities were measured in the three field populations and the results were compared with those of the laboratory susceptible An. stephensi Beech strain. Two separate allele-specific PCR assays were used to identify L1014, L1014F and L1014S mutations in the voltage gated sodium channel gene of An. stephensi. Probit analysis, ANOVA and Hardy–Weinberg equilibrium were used to analyse bioassay, biochemical assay and gene frequency data respectively. Results The population of An. stephensi from Kunar was susceptible to bendiocarb, apart from this, all populations were resistant to all the other insecticides tested. The differences between all values for cytochrome P450s, general esterases, GSTs and AChE inhibition rates in the Kunar, Laghman and Nangarhar populations were statistically significant when compared to the Beech strain, excluding GST activities between Kunar and Beech due to the high standard deviation in Kunar. The three different sodium channel alleles [L1014 (wild type), L1014F (kdr west) and L1014S (kdr east)] were all segregated in the Afghan populations. The frequencies of kdr east mutation were 22.9%, 32.7% and 35% in Kunar, Laghman and Nangarhar populations respectively. Kdr west was at the lowest frequency of 4.44%. Conclusions Resistance to different groups of insecticides in the field populations of An. stephensi from Kunar, Laghman and ... |
format |
Article in Journal/Newspaper |
author |
Noor Halim Zahid Safi Abdul Ali Ahmadi Sami Nahzat Supriya Warusavithana Naimullah Safi Reza Valadan Atie Shemshadian Marzieh Sharifi Ahmadali Enayati Janet Hemingway |
author_facet |
Noor Halim Zahid Safi Abdul Ali Ahmadi Sami Nahzat Supriya Warusavithana Naimullah Safi Reza Valadan Atie Shemshadian Marzieh Sharifi Ahmadali Enayati Janet Hemingway |
author_sort |
Noor Halim Zahid Safi |
title |
Status of insecticide resistance and its biochemical and molecular mechanisms in Anopheles stephensi (Diptera: Culicidae) from Afghanistan |
title_short |
Status of insecticide resistance and its biochemical and molecular mechanisms in Anopheles stephensi (Diptera: Culicidae) from Afghanistan |
title_full |
Status of insecticide resistance and its biochemical and molecular mechanisms in Anopheles stephensi (Diptera: Culicidae) from Afghanistan |
title_fullStr |
Status of insecticide resistance and its biochemical and molecular mechanisms in Anopheles stephensi (Diptera: Culicidae) from Afghanistan |
title_full_unstemmed |
Status of insecticide resistance and its biochemical and molecular mechanisms in Anopheles stephensi (Diptera: Culicidae) from Afghanistan |
title_sort |
status of insecticide resistance and its biochemical and molecular mechanisms in anopheles stephensi (diptera: culicidae) from afghanistan |
publisher |
BMC |
publishDate |
2019 |
url |
https://doi.org/10.1186/s12936-019-2884-x https://doaj.org/article/5edbd6f3a55a4eb690b0aab397a83830 |
long_lat |
ENVELOPE(104.296,104.296,77.666,77.666) |
geographic |
Arctic Kunar |
geographic_facet |
Arctic Kunar |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 18, Iss 1, Pp 1-12 (2019) |
op_relation |
http://link.springer.com/article/10.1186/s12936-019-2884-x https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2884-x 1475-2875 https://doaj.org/article/5edbd6f3a55a4eb690b0aab397a83830 |
op_doi |
https://doi.org/10.1186/s12936-019-2884-x |
container_title |
Malaria Journal |
container_volume |
18 |
container_issue |
1 |
_version_ |
1766346950724026368 |