Identifcation of the Nramp Gene in TV-I fibroblasts from Turbot Psetta maxima, formerly Scophthalmus maximus L. I758 (Pisces: scophthalmidae)
The correct identification of the species of origin in cell culture procedures as well as the detection of eventual cross-contamination by other cells are of essential importance to ensure the correctness of all experimental trials. In this connection, PCR is a fundamental technique to identify the...
Published in: | Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale |
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ftdoajarticles:oai:doaj.org/article:5e8eefc74c3d40a79bc58bd5f2de5392 2023-05-15T18:15:43+02:00 Identifcation of the Nramp Gene in TV-I fibroblasts from Turbot Psetta maxima, formerly Scophthalmus maximus L. I758 (Pisces: scophthalmidae) P. Origone G.L. Mariottini L. Pane 2010-01-01T00:00:00Z https://doi.org/10.4081/jbr.2010.4440 https://doaj.org/article/5e8eefc74c3d40a79bc58bd5f2de5392 EN eng PAGEPress Publications http://www.pagepressjournals.org/index.php/jbr/article/view/4440 https://doaj.org/toc/1826-8838 https://doaj.org/toc/2284-0230 1826-8838 2284-0230 doi:10.4081/jbr.2010.4440 https://doaj.org/article/5e8eefc74c3d40a79bc58bd5f2de5392 Journal of Biological Research, Vol 83, Iss 1 (2010) Turbot Psetta maxima PCR TV-I cells Biology (General) QH301-705.5 article 2010 ftdoajarticles https://doi.org/10.4081/jbr.2010.4440 2022-12-30T23:59:37Z The correct identification of the species of origin in cell culture procedures as well as the detection of eventual cross-contamination by other cells are of essential importance to ensure the correctness of all experimental trials. In this connection, PCR is a fundamental technique to identify the occurrence of genetic information belonging to a defined species. In this paper the correspondence between the known sequences for turbot Psetta maxima (formerly Scophthalmus maximus) and those obtained from TV-I cells from juvenile Psetta maxima was searched by PCR by analysing the Nramp (Natural Resistance Associated Macrophage Protein) gene, which is known to occur in several tissues of turbot and in a turbot embrional cell line. The obtained results showed an high degree of homology of the amplified fragment of TV-I cell line with the Nramp coding region of Teleosts, and a high degree of probability of the occurrence of mRNA belonging to Psetta maxima; so it could be excluded at a greater extent a contamination by genetic material of other organisms and the occurrence of information coming from Teleosts in TV-I cells was confirmed. Article in Journal/Newspaper Scophthalmus maximus Turbot Directory of Open Access Journals: DOAJ Articles Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale 83 1 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Turbot Psetta maxima PCR TV-I cells Biology (General) QH301-705.5 |
spellingShingle |
Turbot Psetta maxima PCR TV-I cells Biology (General) QH301-705.5 P. Origone G.L. Mariottini L. Pane Identifcation of the Nramp Gene in TV-I fibroblasts from Turbot Psetta maxima, formerly Scophthalmus maximus L. I758 (Pisces: scophthalmidae) |
topic_facet |
Turbot Psetta maxima PCR TV-I cells Biology (General) QH301-705.5 |
description |
The correct identification of the species of origin in cell culture procedures as well as the detection of eventual cross-contamination by other cells are of essential importance to ensure the correctness of all experimental trials. In this connection, PCR is a fundamental technique to identify the occurrence of genetic information belonging to a defined species. In this paper the correspondence between the known sequences for turbot Psetta maxima (formerly Scophthalmus maximus) and those obtained from TV-I cells from juvenile Psetta maxima was searched by PCR by analysing the Nramp (Natural Resistance Associated Macrophage Protein) gene, which is known to occur in several tissues of turbot and in a turbot embrional cell line. The obtained results showed an high degree of homology of the amplified fragment of TV-I cell line with the Nramp coding region of Teleosts, and a high degree of probability of the occurrence of mRNA belonging to Psetta maxima; so it could be excluded at a greater extent a contamination by genetic material of other organisms and the occurrence of information coming from Teleosts in TV-I cells was confirmed. |
format |
Article in Journal/Newspaper |
author |
P. Origone G.L. Mariottini L. Pane |
author_facet |
P. Origone G.L. Mariottini L. Pane |
author_sort |
P. Origone |
title |
Identifcation of the Nramp Gene in TV-I fibroblasts from Turbot Psetta maxima, formerly Scophthalmus maximus L. I758 (Pisces: scophthalmidae) |
title_short |
Identifcation of the Nramp Gene in TV-I fibroblasts from Turbot Psetta maxima, formerly Scophthalmus maximus L. I758 (Pisces: scophthalmidae) |
title_full |
Identifcation of the Nramp Gene in TV-I fibroblasts from Turbot Psetta maxima, formerly Scophthalmus maximus L. I758 (Pisces: scophthalmidae) |
title_fullStr |
Identifcation of the Nramp Gene in TV-I fibroblasts from Turbot Psetta maxima, formerly Scophthalmus maximus L. I758 (Pisces: scophthalmidae) |
title_full_unstemmed |
Identifcation of the Nramp Gene in TV-I fibroblasts from Turbot Psetta maxima, formerly Scophthalmus maximus L. I758 (Pisces: scophthalmidae) |
title_sort |
identifcation of the nramp gene in tv-i fibroblasts from turbot psetta maxima, formerly scophthalmus maximus l. i758 (pisces: scophthalmidae) |
publisher |
PAGEPress Publications |
publishDate |
2010 |
url |
https://doi.org/10.4081/jbr.2010.4440 https://doaj.org/article/5e8eefc74c3d40a79bc58bd5f2de5392 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Journal of Biological Research, Vol 83, Iss 1 (2010) |
op_relation |
http://www.pagepressjournals.org/index.php/jbr/article/view/4440 https://doaj.org/toc/1826-8838 https://doaj.org/toc/2284-0230 1826-8838 2284-0230 doi:10.4081/jbr.2010.4440 https://doaj.org/article/5e8eefc74c3d40a79bc58bd5f2de5392 |
op_doi |
https://doi.org/10.4081/jbr.2010.4440 |
container_title |
Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale |
container_volume |
83 |
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1 |
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1766188909097648128 |