Effect of hypoxia, normoxia and hyperoxia conditions on gill histopathology in two weight groups of beluga (Huso huso)
The influence of dissolved oxygen concentration on gill histopathology of great sturgeon (Huso huso) was evaluated in two weight classes (initial weight 280.9±49.2 g and 1217.9±138.1 g respectively). Oxygen treatments included hypoxia (2-3 mg/l), normoxia (5-6 mg/l) and hyperoxia (9-10 mg/l). The fi...
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University of Guilan
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ftdoajarticles:oai:doaj.org/article:5e5f713434124d1bbf799d67cc5a6245 2023-05-15T15:41:53+02:00 Effect of hypoxia, normoxia and hyperoxia conditions on gill histopathology in two weight groups of beluga (Huso huso) Bagherzadeh Lakani, F. Sattari, M. Sharifpour, I. Kazemi, R. 2013-10-01T00:00:00Z https://doaj.org/article/5e5f713434124d1bbf799d67cc5a6245 EN eng University of Guilan https://cjes.guilan.ac.ir/article_1114.html https://doaj.org/toc/1735-3033 https://doaj.org/toc/1735-3866 1735-3033 1735-3866 https://doaj.org/article/5e5f713434124d1bbf799d67cc5a6245 Caspian Journal of Environmental Sciences, Vol 11, Iss 1, Pp 77-84 (2013) gill hyperoxia normoxia hypoxia sturgeon Fish Environmental sciences GE1-350 Science Q article 2013 ftdoajarticles 2022-12-31T08:45:21Z The influence of dissolved oxygen concentration on gill histopathology of great sturgeon (Huso huso) was evaluated in two weight classes (initial weight 280.9±49.2 g and 1217.9±138.1 g respectively). Oxygen treatments included hypoxia (2-3 mg/l), normoxia (5-6 mg/l) and hyperoxia (9-10 mg/l). The fish were acclimated to experimental tanks for one week then randomly distributed into 9 tanks in each of the initial weight classes (3 and 6 fish per tank in higher and lower initial weight classes respectively) for 8 weeks. In order to find the histopathological changes, gill samples were collected, dehydrated through ethanol series, embedded in paraffin , sectioned at 7 µm thickness using a Leitz microtome and stained with H & E. No mortality was observed over the 8 weeks of the experimental period. There were significant differences in weight and feed intake between treatments in the both weight classes (P<0.05). Fork length showed significant differences in lower initial weight class (P<0.05). The main histopathological changes were observed in gills including: Hyperplasia, loss of secondary lamellae, hemorrhage and congestion in primary and secondary lamellae, lamellar fusion, epithelial lifting in secondary lamellae, clubbing of secondary lamellae, telangiectases, increase in melanin pigments and numerous vacuoles in primary and secondary lamellae (in hyperoxia treatment). All these lesions may reduce gill functional surface of gaseous exchange, impairing respiratory function. Article in Journal/Newspaper Beluga Beluga* Directory of Open Access Journals: DOAJ Articles |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
gill hyperoxia normoxia hypoxia sturgeon Fish Environmental sciences GE1-350 Science Q |
spellingShingle |
gill hyperoxia normoxia hypoxia sturgeon Fish Environmental sciences GE1-350 Science Q Bagherzadeh Lakani, F. Sattari, M. Sharifpour, I. Kazemi, R. Effect of hypoxia, normoxia and hyperoxia conditions on gill histopathology in two weight groups of beluga (Huso huso) |
topic_facet |
gill hyperoxia normoxia hypoxia sturgeon Fish Environmental sciences GE1-350 Science Q |
description |
The influence of dissolved oxygen concentration on gill histopathology of great sturgeon (Huso huso) was evaluated in two weight classes (initial weight 280.9±49.2 g and 1217.9±138.1 g respectively). Oxygen treatments included hypoxia (2-3 mg/l), normoxia (5-6 mg/l) and hyperoxia (9-10 mg/l). The fish were acclimated to experimental tanks for one week then randomly distributed into 9 tanks in each of the initial weight classes (3 and 6 fish per tank in higher and lower initial weight classes respectively) for 8 weeks. In order to find the histopathological changes, gill samples were collected, dehydrated through ethanol series, embedded in paraffin , sectioned at 7 µm thickness using a Leitz microtome and stained with H & E. No mortality was observed over the 8 weeks of the experimental period. There were significant differences in weight and feed intake between treatments in the both weight classes (P<0.05). Fork length showed significant differences in lower initial weight class (P<0.05). The main histopathological changes were observed in gills including: Hyperplasia, loss of secondary lamellae, hemorrhage and congestion in primary and secondary lamellae, lamellar fusion, epithelial lifting in secondary lamellae, clubbing of secondary lamellae, telangiectases, increase in melanin pigments and numerous vacuoles in primary and secondary lamellae (in hyperoxia treatment). All these lesions may reduce gill functional surface of gaseous exchange, impairing respiratory function. |
format |
Article in Journal/Newspaper |
author |
Bagherzadeh Lakani, F. Sattari, M. Sharifpour, I. Kazemi, R. |
author_facet |
Bagherzadeh Lakani, F. Sattari, M. Sharifpour, I. Kazemi, R. |
author_sort |
Bagherzadeh Lakani, F. |
title |
Effect of hypoxia, normoxia and hyperoxia conditions on gill histopathology in two weight groups of beluga (Huso huso) |
title_short |
Effect of hypoxia, normoxia and hyperoxia conditions on gill histopathology in two weight groups of beluga (Huso huso) |
title_full |
Effect of hypoxia, normoxia and hyperoxia conditions on gill histopathology in two weight groups of beluga (Huso huso) |
title_fullStr |
Effect of hypoxia, normoxia and hyperoxia conditions on gill histopathology in two weight groups of beluga (Huso huso) |
title_full_unstemmed |
Effect of hypoxia, normoxia and hyperoxia conditions on gill histopathology in two weight groups of beluga (Huso huso) |
title_sort |
effect of hypoxia, normoxia and hyperoxia conditions on gill histopathology in two weight groups of beluga (huso huso) |
publisher |
University of Guilan |
publishDate |
2013 |
url |
https://doaj.org/article/5e5f713434124d1bbf799d67cc5a6245 |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_source |
Caspian Journal of Environmental Sciences, Vol 11, Iss 1, Pp 77-84 (2013) |
op_relation |
https://cjes.guilan.ac.ir/article_1114.html https://doaj.org/toc/1735-3033 https://doaj.org/toc/1735-3866 1735-3033 1735-3866 https://doaj.org/article/5e5f713434124d1bbf799d67cc5a6245 |
_version_ |
1766374773162508288 |