Genome-Wide DNA Methylation in Policemen Working in Cities Differing by Major Sources of Air Pollution
DNA methylation is the most studied epigenetic mechanism that regulates gene expression, and it can serve as a useful biomarker of prior environmental exposure and future health outcomes. This study focused on DNA methylation profiles in a human cohort, comprising 125 nonsmoking city policemen (samp...
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ftdoajarticles:oai:doaj.org/article:5e1e7a6ffe04414b994ae0c6b6eab045 2023-05-15T16:01:29+02:00 Genome-Wide DNA Methylation in Policemen Working in Cities Differing by Major Sources of Air Pollution Katerina Honkova Andrea Rossnerova Irena Chvojkova Alena Milcova Hasmik Margaryan Anna Pastorkova Antonin Ambroz Pavel Rossner Vitezslav Jirik Jiri Rubes Radim J. Sram Jan Topinka 2022-01-01T00:00:00Z https://doi.org/10.3390/ijms23031666 https://doaj.org/article/5e1e7a6ffe04414b994ae0c6b6eab045 EN eng MDPI AG https://www.mdpi.com/1422-0067/23/3/1666 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms23031666 1422-0067 1661-6596 https://doaj.org/article/5e1e7a6ffe04414b994ae0c6b6eab045 International Journal of Molecular Sciences, Vol 23, Iss 1666, p 1666 (2022) air pollution DNA methylation environment molecular epidemiology epigenetics Biology (General) QH301-705.5 Chemistry QD1-999 article 2022 ftdoajarticles https://doi.org/10.3390/ijms23031666 2022-12-31T15:19:51Z DNA methylation is the most studied epigenetic mechanism that regulates gene expression, and it can serve as a useful biomarker of prior environmental exposure and future health outcomes. This study focused on DNA methylation profiles in a human cohort, comprising 125 nonsmoking city policemen (sampled twice), living and working in three localities (Prague, Ostrava and Ceske Budejovice) of the Czech Republic, who spent the majority of their working time outdoors. The main characterization of the localities, differing by major sources of air pollution, was defined by the stationary air pollution monitoring of PM2.5, B[a]P and NO 2 . DNA methylation was analyzed by a genome-wide microarray method. No season-specific DNA methylation pattern was discovered; however, we identified 13,643 differentially methylated CpG loci (DML) for a comparison between the Prague and Ostrava groups. The most significant DML was cg10123377 (log 2 FC = −1.92, p = 8.30 × 10 −4 ) and loci annotated to RPTOR (total 20 CpG loci). We also found two hypomethylated loci annotated to the DNA repair gene XRCC5. Groups of DML annotated to the same gene were linked to diabetes mellitus ( KCNQ1 ), respiratory diseases ( PTPRN2 ), the dopaminergic system of the brain and neurodegenerative diseases ( NR4A2 ). The most significant possibly affected pathway was Axon guidance, with 86 potentially deregulated genes near DML. The cluster of gene sets that could be affected by DNA methylation in the Ostrava groups mainly includes the neuronal functions and biological processes of cell junctions and adhesion assembly. The study demonstrates that the differences in the type of air pollution between localities can affect a unique change in DNA methylation profiles across the human genome. Article in Journal/Newspaper DML Directory of Open Access Journals: DOAJ Articles International Journal of Molecular Sciences 23 3 1666 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
air pollution DNA methylation environment molecular epidemiology epigenetics Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
air pollution DNA methylation environment molecular epidemiology epigenetics Biology (General) QH301-705.5 Chemistry QD1-999 Katerina Honkova Andrea Rossnerova Irena Chvojkova Alena Milcova Hasmik Margaryan Anna Pastorkova Antonin Ambroz Pavel Rossner Vitezslav Jirik Jiri Rubes Radim J. Sram Jan Topinka Genome-Wide DNA Methylation in Policemen Working in Cities Differing by Major Sources of Air Pollution |
topic_facet |
air pollution DNA methylation environment molecular epidemiology epigenetics Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
DNA methylation is the most studied epigenetic mechanism that regulates gene expression, and it can serve as a useful biomarker of prior environmental exposure and future health outcomes. This study focused on DNA methylation profiles in a human cohort, comprising 125 nonsmoking city policemen (sampled twice), living and working in three localities (Prague, Ostrava and Ceske Budejovice) of the Czech Republic, who spent the majority of their working time outdoors. The main characterization of the localities, differing by major sources of air pollution, was defined by the stationary air pollution monitoring of PM2.5, B[a]P and NO 2 . DNA methylation was analyzed by a genome-wide microarray method. No season-specific DNA methylation pattern was discovered; however, we identified 13,643 differentially methylated CpG loci (DML) for a comparison between the Prague and Ostrava groups. The most significant DML was cg10123377 (log 2 FC = −1.92, p = 8.30 × 10 −4 ) and loci annotated to RPTOR (total 20 CpG loci). We also found two hypomethylated loci annotated to the DNA repair gene XRCC5. Groups of DML annotated to the same gene were linked to diabetes mellitus ( KCNQ1 ), respiratory diseases ( PTPRN2 ), the dopaminergic system of the brain and neurodegenerative diseases ( NR4A2 ). The most significant possibly affected pathway was Axon guidance, with 86 potentially deregulated genes near DML. The cluster of gene sets that could be affected by DNA methylation in the Ostrava groups mainly includes the neuronal functions and biological processes of cell junctions and adhesion assembly. The study demonstrates that the differences in the type of air pollution between localities can affect a unique change in DNA methylation profiles across the human genome. |
format |
Article in Journal/Newspaper |
author |
Katerina Honkova Andrea Rossnerova Irena Chvojkova Alena Milcova Hasmik Margaryan Anna Pastorkova Antonin Ambroz Pavel Rossner Vitezslav Jirik Jiri Rubes Radim J. Sram Jan Topinka |
author_facet |
Katerina Honkova Andrea Rossnerova Irena Chvojkova Alena Milcova Hasmik Margaryan Anna Pastorkova Antonin Ambroz Pavel Rossner Vitezslav Jirik Jiri Rubes Radim J. Sram Jan Topinka |
author_sort |
Katerina Honkova |
title |
Genome-Wide DNA Methylation in Policemen Working in Cities Differing by Major Sources of Air Pollution |
title_short |
Genome-Wide DNA Methylation in Policemen Working in Cities Differing by Major Sources of Air Pollution |
title_full |
Genome-Wide DNA Methylation in Policemen Working in Cities Differing by Major Sources of Air Pollution |
title_fullStr |
Genome-Wide DNA Methylation in Policemen Working in Cities Differing by Major Sources of Air Pollution |
title_full_unstemmed |
Genome-Wide DNA Methylation in Policemen Working in Cities Differing by Major Sources of Air Pollution |
title_sort |
genome-wide dna methylation in policemen working in cities differing by major sources of air pollution |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/ijms23031666 https://doaj.org/article/5e1e7a6ffe04414b994ae0c6b6eab045 |
genre |
DML |
genre_facet |
DML |
op_source |
International Journal of Molecular Sciences, Vol 23, Iss 1666, p 1666 (2022) |
op_relation |
https://www.mdpi.com/1422-0067/23/3/1666 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms23031666 1422-0067 1661-6596 https://doaj.org/article/5e1e7a6ffe04414b994ae0c6b6eab045 |
op_doi |
https://doi.org/10.3390/ijms23031666 |
container_title |
International Journal of Molecular Sciences |
container_volume |
23 |
container_issue |
3 |
container_start_page |
1666 |
_version_ |
1766397313373175808 |