Amino acid metabolic signaling influences Aedes aegypti midgut microbiome variability.
The mosquito midgut microbiota has been shown to influence vector competence for multiple human pathogens. The microbiota is highly variable in the field, and the sources of this variability are not well understood, which limits our ability to understand or predict its effects on pathogen transmissi...
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ftdoajarticles:oai:doaj.org/article:5e1dba918be6418fa5578ccb0ff97743 2023-05-15T15:10:25+02:00 Amino acid metabolic signaling influences Aedes aegypti midgut microbiome variability. Sarah M Short Emmanuel F Mongodin Hannah J MacLeod Octavio A C Talyuli George Dimopoulos 2017-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005677 https://doaj.org/article/5e1dba918be6418fa5578ccb0ff97743 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5549995?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005677 https://doaj.org/article/5e1dba918be6418fa5578ccb0ff97743 PLoS Neglected Tropical Diseases, Vol 11, Iss 7, p e0005677 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005677 2022-12-31T04:15:01Z The mosquito midgut microbiota has been shown to influence vector competence for multiple human pathogens. The microbiota is highly variable in the field, and the sources of this variability are not well understood, which limits our ability to understand or predict its effects on pathogen transmission. In this work, we report significant variation in female adult midgut bacterial load between strains of A. aegypti which vary in their susceptibility to dengue virus. Composition of the midgut microbiome was similar overall between the strains, with 81-92% of reads coming from the same five bacterial families, though we did detect differences in the presence of some bacterial families including Flavobacteriaceae and Entobacteriaceae. We conducted transcriptomic analysis on the two mosquito strains that showed the greatest difference in bacterial load, and found that they differ in transcript abundance of many genes implicated in amino acid metabolism, in particular the branched chain amino acid degradation pathway. We then silenced this pathway by targeting multiple genes using RNA interference, which resulted in strain-specific bacterial proliferation, thereby eliminating the difference in midgut bacterial load between the strains. This suggests that the branched chain amino acid (BCAA) degradation pathway controls midgut bacterial load, though the mechanism underlying this remains unclear. Overall, our results indicate that amino acid metabolism can act to influence the midgut microbiota. Moreover, they suggest that genetic or physiological variation in BCAA degradation pathway activity may in part explain midgut microbiota variation in the field. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 7 e0005677 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Sarah M Short Emmanuel F Mongodin Hannah J MacLeod Octavio A C Talyuli George Dimopoulos Amino acid metabolic signaling influences Aedes aegypti midgut microbiome variability. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The mosquito midgut microbiota has been shown to influence vector competence for multiple human pathogens. The microbiota is highly variable in the field, and the sources of this variability are not well understood, which limits our ability to understand or predict its effects on pathogen transmission. In this work, we report significant variation in female adult midgut bacterial load between strains of A. aegypti which vary in their susceptibility to dengue virus. Composition of the midgut microbiome was similar overall between the strains, with 81-92% of reads coming from the same five bacterial families, though we did detect differences in the presence of some bacterial families including Flavobacteriaceae and Entobacteriaceae. We conducted transcriptomic analysis on the two mosquito strains that showed the greatest difference in bacterial load, and found that they differ in transcript abundance of many genes implicated in amino acid metabolism, in particular the branched chain amino acid degradation pathway. We then silenced this pathway by targeting multiple genes using RNA interference, which resulted in strain-specific bacterial proliferation, thereby eliminating the difference in midgut bacterial load between the strains. This suggests that the branched chain amino acid (BCAA) degradation pathway controls midgut bacterial load, though the mechanism underlying this remains unclear. Overall, our results indicate that amino acid metabolism can act to influence the midgut microbiota. Moreover, they suggest that genetic or physiological variation in BCAA degradation pathway activity may in part explain midgut microbiota variation in the field. |
format |
Article in Journal/Newspaper |
author |
Sarah M Short Emmanuel F Mongodin Hannah J MacLeod Octavio A C Talyuli George Dimopoulos |
author_facet |
Sarah M Short Emmanuel F Mongodin Hannah J MacLeod Octavio A C Talyuli George Dimopoulos |
author_sort |
Sarah M Short |
title |
Amino acid metabolic signaling influences Aedes aegypti midgut microbiome variability. |
title_short |
Amino acid metabolic signaling influences Aedes aegypti midgut microbiome variability. |
title_full |
Amino acid metabolic signaling influences Aedes aegypti midgut microbiome variability. |
title_fullStr |
Amino acid metabolic signaling influences Aedes aegypti midgut microbiome variability. |
title_full_unstemmed |
Amino acid metabolic signaling influences Aedes aegypti midgut microbiome variability. |
title_sort |
amino acid metabolic signaling influences aedes aegypti midgut microbiome variability. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005677 https://doaj.org/article/5e1dba918be6418fa5578ccb0ff97743 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 7, p e0005677 (2017) |
op_relation |
http://europepmc.org/articles/PMC5549995?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005677 https://doaj.org/article/5e1dba918be6418fa5578ccb0ff97743 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005677 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
7 |
container_start_page |
e0005677 |
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1766341456089317376 |