α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model.
Chagas disease, caused by the parasite Trypanosoma cruzi, is considered endemic in more than 20 countries but lacks both an approved vaccine and limited treatment for its chronic stage. Chronic infection is most harmful to human health because of long-term parasitic infection of the heart. Here we s...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2021
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0009613 https://doaj.org/article/5e151268acdb46dfbf28a814c81a6e28 |
id |
ftdoajarticles:oai:doaj.org/article:5e151268acdb46dfbf28a814c81a6e28 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:5e151268acdb46dfbf28a814c81a6e28 2023-05-15T15:05:43+02:00 α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model. Gisele Macêdo Rodrigues da Cunha Maíra Araújo Azevedo Denise Silva Nogueira Marianna de Carvalho Clímaco Edward Valencia Ayala Juan Atilio Jimenez Chunga Raul Jesus Ynocente La Valle Lucia Maria da Cunha Galvão Egler Chiari Carlos Ramon Nascimento Brito Rodrigo Pedro Soares Paula Monalisa Nogueira Ricardo Toshio Fujiwara Ricardo Gazzinelli Robert Hincapie Carlos-Sanhueza Chaves Fabricio Marcus Silva Oliveira M G Finn Alexandre Ferreira Marques 2021-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009613 https://doaj.org/article/5e151268acdb46dfbf28a814c81a6e28 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009613 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009613 https://doaj.org/article/5e151268acdb46dfbf28a814c81a6e28 PLoS Neglected Tropical Diseases, Vol 15, Iss 7, p e0009613 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009613 2022-12-31T16:22:38Z Chagas disease, caused by the parasite Trypanosoma cruzi, is considered endemic in more than 20 countries but lacks both an approved vaccine and limited treatment for its chronic stage. Chronic infection is most harmful to human health because of long-term parasitic infection of the heart. Here we show that immunization with a virus-like particle vaccine displaying a high density of the immunogenic α-Gal trisaccharide (Qβ-αGal) induced several beneficial effects concerning acute and chronic T. cruzi infection in α1,3-galactosyltransferase knockout mice. Approximately 60% of these animals were protected from initial infection with high parasite loads. Vaccinated animals also produced high anti-αGal IgG antibody titers, improved IFN-γ and IL-12 cytokine production, and controlled parasitemia in the acute phase at 8 days post-infection (dpi) for the Y strain and 22 dpi for the Colombian strain. In the chronic stage of infection (36 and 190 dpi, respectively), all of the vaccinated group survived, showing significantly decreased heart inflammation and clearance of amastigote nests from the heart tissue. Article in Journal/Newspaper Arctic Human health Directory of Open Access Journals: DOAJ Articles Arctic The ''Y'' ENVELOPE(-112.453,-112.453,57.591,57.591) PLOS Neglected Tropical Diseases 15 7 e0009613 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Gisele Macêdo Rodrigues da Cunha Maíra Araújo Azevedo Denise Silva Nogueira Marianna de Carvalho Clímaco Edward Valencia Ayala Juan Atilio Jimenez Chunga Raul Jesus Ynocente La Valle Lucia Maria da Cunha Galvão Egler Chiari Carlos Ramon Nascimento Brito Rodrigo Pedro Soares Paula Monalisa Nogueira Ricardo Toshio Fujiwara Ricardo Gazzinelli Robert Hincapie Carlos-Sanhueza Chaves Fabricio Marcus Silva Oliveira M G Finn Alexandre Ferreira Marques α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Chagas disease, caused by the parasite Trypanosoma cruzi, is considered endemic in more than 20 countries but lacks both an approved vaccine and limited treatment for its chronic stage. Chronic infection is most harmful to human health because of long-term parasitic infection of the heart. Here we show that immunization with a virus-like particle vaccine displaying a high density of the immunogenic α-Gal trisaccharide (Qβ-αGal) induced several beneficial effects concerning acute and chronic T. cruzi infection in α1,3-galactosyltransferase knockout mice. Approximately 60% of these animals were protected from initial infection with high parasite loads. Vaccinated animals also produced high anti-αGal IgG antibody titers, improved IFN-γ and IL-12 cytokine production, and controlled parasitemia in the acute phase at 8 days post-infection (dpi) for the Y strain and 22 dpi for the Colombian strain. In the chronic stage of infection (36 and 190 dpi, respectively), all of the vaccinated group survived, showing significantly decreased heart inflammation and clearance of amastigote nests from the heart tissue. |
format |
Article in Journal/Newspaper |
author |
Gisele Macêdo Rodrigues da Cunha Maíra Araújo Azevedo Denise Silva Nogueira Marianna de Carvalho Clímaco Edward Valencia Ayala Juan Atilio Jimenez Chunga Raul Jesus Ynocente La Valle Lucia Maria da Cunha Galvão Egler Chiari Carlos Ramon Nascimento Brito Rodrigo Pedro Soares Paula Monalisa Nogueira Ricardo Toshio Fujiwara Ricardo Gazzinelli Robert Hincapie Carlos-Sanhueza Chaves Fabricio Marcus Silva Oliveira M G Finn Alexandre Ferreira Marques |
author_facet |
Gisele Macêdo Rodrigues da Cunha Maíra Araújo Azevedo Denise Silva Nogueira Marianna de Carvalho Clímaco Edward Valencia Ayala Juan Atilio Jimenez Chunga Raul Jesus Ynocente La Valle Lucia Maria da Cunha Galvão Egler Chiari Carlos Ramon Nascimento Brito Rodrigo Pedro Soares Paula Monalisa Nogueira Ricardo Toshio Fujiwara Ricardo Gazzinelli Robert Hincapie Carlos-Sanhueza Chaves Fabricio Marcus Silva Oliveira M G Finn Alexandre Ferreira Marques |
author_sort |
Gisele Macêdo Rodrigues da Cunha |
title |
α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model. |
title_short |
α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model. |
title_full |
α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model. |
title_fullStr |
α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model. |
title_full_unstemmed |
α-Gal immunization positively impacts Trypanosoma cruzi colonization of heart tissue in a mouse model. |
title_sort |
α-gal immunization positively impacts trypanosoma cruzi colonization of heart tissue in a mouse model. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009613 https://doaj.org/article/5e151268acdb46dfbf28a814c81a6e28 |
long_lat |
ENVELOPE(-112.453,-112.453,57.591,57.591) |
geographic |
Arctic The ''Y'' |
geographic_facet |
Arctic The ''Y'' |
genre |
Arctic Human health |
genre_facet |
Arctic Human health |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 7, p e0009613 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009613 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009613 https://doaj.org/article/5e151268acdb46dfbf28a814c81a6e28 |
op_doi |
https://doi.org/10.1371/journal.pntd.0009613 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
15 |
container_issue |
7 |
container_start_page |
e0009613 |
_version_ |
1766337364884455424 |