Reliability of serological methods for detection of leishmaniasis in portuguese domestic and wild reservoirs
A direct agglutination test (DAT) and an immunofluorescence technique (IFAT) were compared for detection of Leishmania infantum infection in 43 dogs and five foxes from Alto-Douro and Arrábida, two known endemic areas in Portugal. In four dogs with proved canine leishmaniasis, both DAT and IFAT show...
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Instituto Oswaldo Cruz, Ministério da Saúde
1996
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Online Access: | https://doi.org/10.1590/S0074-02761996000600018 https://doaj.org/article/5d6927a0cc9740bf86f78f61dc544703 |
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ftdoajarticles:oai:doaj.org/article:5d6927a0cc9740bf86f78f61dc544703 2023-05-15T15:04:07+02:00 Reliability of serological methods for detection of leishmaniasis in portuguese domestic and wild reservoirs SJ Semião-Santos P Abranches MCD Silva-Pereira GM Santos-Gomes JP Fernandes JCM Vetter 1996-12-01T00:00:00Z https://doi.org/10.1590/S0074-02761996000600018 https://doaj.org/article/5d6927a0cc9740bf86f78f61dc544703 EN eng Instituto Oswaldo Cruz, Ministério da Saúde http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000600018 https://doaj.org/toc/0074-0276 https://doaj.org/toc/1678-8060 doi:10.1590/S0074-02761996000600018 0074-0276 1678-8060 https://doaj.org/article/5d6927a0cc9740bf86f78f61dc544703 Memórias do Instituto Oswaldo Cruz., Vol 91, Iss 6, Pp 747-750 (1996) canine vulpine leishmaniasis direct agglutination test immunofluorescence technique Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 article 1996 ftdoajarticles https://doi.org/10.1590/S0074-02761996000600018 2023-01-08T01:24:11Z A direct agglutination test (DAT) and an immunofluorescence technique (IFAT) were compared for detection of Leishmania infantum infection in 43 dogs and five foxes from Alto-Douro and Arrábida, two known endemic areas in Portugal. In four dogs with proved canine leishmaniasis, both DAT and IFAT showed positive readings (titres >1:320 and >1:128). Of 34 samples collected from apparently healthy dogs, ten were positive by both serological tests and eight were serologically positive by one test or the other. Three foxes out of five captured in this area, scored titres indicative of leishmaniasis in both DAT and IFAT. The concordance between DAT and IFAT in all collected samples (48) was 81.25%. Considering these and previous studies in the adjacent Mediterranean areas, the seroprevalence of L. infantum infection in the canine and vulpine populations appear to be of high magnitude. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Memórias do Instituto Oswaldo Cruz 91 6 747 750 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
canine vulpine leishmaniasis direct agglutination test immunofluorescence technique Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 |
spellingShingle |
canine vulpine leishmaniasis direct agglutination test immunofluorescence technique Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 SJ Semião-Santos P Abranches MCD Silva-Pereira GM Santos-Gomes JP Fernandes JCM Vetter Reliability of serological methods for detection of leishmaniasis in portuguese domestic and wild reservoirs |
topic_facet |
canine vulpine leishmaniasis direct agglutination test immunofluorescence technique Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 |
description |
A direct agglutination test (DAT) and an immunofluorescence technique (IFAT) were compared for detection of Leishmania infantum infection in 43 dogs and five foxes from Alto-Douro and Arrábida, two known endemic areas in Portugal. In four dogs with proved canine leishmaniasis, both DAT and IFAT showed positive readings (titres >1:320 and >1:128). Of 34 samples collected from apparently healthy dogs, ten were positive by both serological tests and eight were serologically positive by one test or the other. Three foxes out of five captured in this area, scored titres indicative of leishmaniasis in both DAT and IFAT. The concordance between DAT and IFAT in all collected samples (48) was 81.25%. Considering these and previous studies in the adjacent Mediterranean areas, the seroprevalence of L. infantum infection in the canine and vulpine populations appear to be of high magnitude. |
format |
Article in Journal/Newspaper |
author |
SJ Semião-Santos P Abranches MCD Silva-Pereira GM Santos-Gomes JP Fernandes JCM Vetter |
author_facet |
SJ Semião-Santos P Abranches MCD Silva-Pereira GM Santos-Gomes JP Fernandes JCM Vetter |
author_sort |
SJ Semião-Santos |
title |
Reliability of serological methods for detection of leishmaniasis in portuguese domestic and wild reservoirs |
title_short |
Reliability of serological methods for detection of leishmaniasis in portuguese domestic and wild reservoirs |
title_full |
Reliability of serological methods for detection of leishmaniasis in portuguese domestic and wild reservoirs |
title_fullStr |
Reliability of serological methods for detection of leishmaniasis in portuguese domestic and wild reservoirs |
title_full_unstemmed |
Reliability of serological methods for detection of leishmaniasis in portuguese domestic and wild reservoirs |
title_sort |
reliability of serological methods for detection of leishmaniasis in portuguese domestic and wild reservoirs |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
1996 |
url |
https://doi.org/10.1590/S0074-02761996000600018 https://doaj.org/article/5d6927a0cc9740bf86f78f61dc544703 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Memórias do Instituto Oswaldo Cruz., Vol 91, Iss 6, Pp 747-750 (1996) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000600018 https://doaj.org/toc/0074-0276 https://doaj.org/toc/1678-8060 doi:10.1590/S0074-02761996000600018 0074-0276 1678-8060 https://doaj.org/article/5d6927a0cc9740bf86f78f61dc544703 |
op_doi |
https://doi.org/10.1590/S0074-02761996000600018 |
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Memórias do Instituto Oswaldo Cruz |
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91 |
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