Hydrogen utilization potential in subsurface sediments
Subsurface microbial communities undertake many terminal electron-accepting processes, often simultaneously. Using a tritium-based assay, we measured the potential hydrogen oxidation catalyzed by hydrogenase enzymes in several subsurface sedimentary environments (Lake Van, Barents Sea, Equatorial Pa...
Published in: | Frontiers in Microbiology |
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Online Access: | https://doi.org/10.3389/fmicb.2016.00008 https://doaj.org/article/5d5c91404fb24af2bb7c8866c1490008 |
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ftdoajarticles:oai:doaj.org/article:5d5c91404fb24af2bb7c8866c1490008 2023-05-15T15:39:01+02:00 Hydrogen utilization potential in subsurface sediments Rishi Ram Adhikari Clemens eGlombitza Julia C Nickel Chloe H Anderson Ann G Dunlea Arthur J Spivack Richard W Murray Steven eD'Hondt Jens eKallmeyer 2016-01-01T00:00:00Z https://doi.org/10.3389/fmicb.2016.00008 https://doaj.org/article/5d5c91404fb24af2bb7c8866c1490008 EN eng Frontiers Media S.A. http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00008/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2016.00008 https://doaj.org/article/5d5c91404fb24af2bb7c8866c1490008 Frontiers in Microbiology, Vol 7 (2016) Gulf of Mexico Hydrogenase deep biosphere microbial activity Lake Van equatorial Pacific Microbiology QR1-502 article 2016 ftdoajarticles https://doi.org/10.3389/fmicb.2016.00008 2022-12-31T01:09:55Z Subsurface microbial communities undertake many terminal electron-accepting processes, often simultaneously. Using a tritium-based assay, we measured the potential hydrogen oxidation catalyzed by hydrogenase enzymes in several subsurface sedimentary environments (Lake Van, Barents Sea, Equatorial Pacific and Gulf of Mexico) with different predominant electron-acceptors. Hydrogenases constitute a diverse family of enzymes expressed by microorganisms that utilize molecular hydrogen as a metabolic substrate, product or intermediate. The assay reveals the potential for utilizing molecular hydrogen and allows qualitative detection of microbial activity irrespective of the predominant electron-accepting process. Because the method only requires samples frozen immediately after recovery, the assay can be used for identifying microbial activity in subsurface ecosystems without the need to preserve live material.We measured potential hydrogen oxidation rates in all samples from multiple depths at several sites that collectively span a wide range of environmental conditions and biogeochemical zones. Potential activity normalized to total cell abundance ranges over five orders of magnitude and varies, dependent upon the predominant terminal electron acceptor. Lowest per-cell potential rates characterize the zone of nitrate reduction and highest per-cell potential rates occur in the methanogenic zone. Possible reasons for this relationship to predominant electron acceptor include (i) increasing importance of fermentation in successively deeper biogeochemical zones and (ii) adaptation of H2ases to successively higher concentrations of H2 in successively deeper zones. Article in Journal/Newspaper Barents Sea Directory of Open Access Journals: DOAJ Articles Barents Sea Pacific Frontiers in Microbiology 7 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Gulf of Mexico Hydrogenase deep biosphere microbial activity Lake Van equatorial Pacific Microbiology QR1-502 |
spellingShingle |
Gulf of Mexico Hydrogenase deep biosphere microbial activity Lake Van equatorial Pacific Microbiology QR1-502 Rishi Ram Adhikari Clemens eGlombitza Julia C Nickel Chloe H Anderson Ann G Dunlea Arthur J Spivack Richard W Murray Steven eD'Hondt Jens eKallmeyer Hydrogen utilization potential in subsurface sediments |
topic_facet |
Gulf of Mexico Hydrogenase deep biosphere microbial activity Lake Van equatorial Pacific Microbiology QR1-502 |
description |
Subsurface microbial communities undertake many terminal electron-accepting processes, often simultaneously. Using a tritium-based assay, we measured the potential hydrogen oxidation catalyzed by hydrogenase enzymes in several subsurface sedimentary environments (Lake Van, Barents Sea, Equatorial Pacific and Gulf of Mexico) with different predominant electron-acceptors. Hydrogenases constitute a diverse family of enzymes expressed by microorganisms that utilize molecular hydrogen as a metabolic substrate, product or intermediate. The assay reveals the potential for utilizing molecular hydrogen and allows qualitative detection of microbial activity irrespective of the predominant electron-accepting process. Because the method only requires samples frozen immediately after recovery, the assay can be used for identifying microbial activity in subsurface ecosystems without the need to preserve live material.We measured potential hydrogen oxidation rates in all samples from multiple depths at several sites that collectively span a wide range of environmental conditions and biogeochemical zones. Potential activity normalized to total cell abundance ranges over five orders of magnitude and varies, dependent upon the predominant terminal electron acceptor. Lowest per-cell potential rates characterize the zone of nitrate reduction and highest per-cell potential rates occur in the methanogenic zone. Possible reasons for this relationship to predominant electron acceptor include (i) increasing importance of fermentation in successively deeper biogeochemical zones and (ii) adaptation of H2ases to successively higher concentrations of H2 in successively deeper zones. |
format |
Article in Journal/Newspaper |
author |
Rishi Ram Adhikari Clemens eGlombitza Julia C Nickel Chloe H Anderson Ann G Dunlea Arthur J Spivack Richard W Murray Steven eD'Hondt Jens eKallmeyer |
author_facet |
Rishi Ram Adhikari Clemens eGlombitza Julia C Nickel Chloe H Anderson Ann G Dunlea Arthur J Spivack Richard W Murray Steven eD'Hondt Jens eKallmeyer |
author_sort |
Rishi Ram Adhikari |
title |
Hydrogen utilization potential in subsurface sediments |
title_short |
Hydrogen utilization potential in subsurface sediments |
title_full |
Hydrogen utilization potential in subsurface sediments |
title_fullStr |
Hydrogen utilization potential in subsurface sediments |
title_full_unstemmed |
Hydrogen utilization potential in subsurface sediments |
title_sort |
hydrogen utilization potential in subsurface sediments |
publisher |
Frontiers Media S.A. |
publishDate |
2016 |
url |
https://doi.org/10.3389/fmicb.2016.00008 https://doaj.org/article/5d5c91404fb24af2bb7c8866c1490008 |
geographic |
Barents Sea Pacific |
geographic_facet |
Barents Sea Pacific |
genre |
Barents Sea |
genre_facet |
Barents Sea |
op_source |
Frontiers in Microbiology, Vol 7 (2016) |
op_relation |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00008/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2016.00008 https://doaj.org/article/5d5c91404fb24af2bb7c8866c1490008 |
op_doi |
https://doi.org/10.3389/fmicb.2016.00008 |
container_title |
Frontiers in Microbiology |
container_volume |
7 |
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1766370475231936512 |