Spatial and temporal variability in the response of phytoplankton and prokaryotes to B-vitamin amendments in an upwelling system
We experimentally evaluated the temporal (interday and interseason) and spatial variability in microbial plankton responses to vitamin B12 and/or B1 supply (solely or in combination with inorganic nutrients) in coastal and oceanic waters of the northeast Atlantic Ocean. Phytoplankton and, to a lesse...
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2020
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ftdoajarticles:oai:doaj.org/article:5d54b03b82834f9dad805b28951ea04c 2023-05-15T17:41:38+02:00 Spatial and temporal variability in the response of phytoplankton and prokaryotes to B-vitamin amendments in an upwelling system V. Joglar A. Prieto E. Barber-Lluch M. Hernández-Ruiz E. Fernández E. Teira 2020-05-01T00:00:00Z https://doi.org/10.5194/bg-17-2807-2020 https://doaj.org/article/5d54b03b82834f9dad805b28951ea04c EN eng Copernicus Publications https://www.biogeosciences.net/17/2807/2020/bg-17-2807-2020.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 doi:10.5194/bg-17-2807-2020 1726-4170 1726-4189 https://doaj.org/article/5d54b03b82834f9dad805b28951ea04c Biogeosciences, Vol 17, Pp 2807-2823 (2020) Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 article 2020 ftdoajarticles https://doi.org/10.5194/bg-17-2807-2020 2022-12-30T22:00:29Z We experimentally evaluated the temporal (interday and interseason) and spatial variability in microbial plankton responses to vitamin B12 and/or B1 supply (solely or in combination with inorganic nutrients) in coastal and oceanic waters of the northeast Atlantic Ocean. Phytoplankton and, to a lesser extent, prokaryotes were strongly limited by inorganic nutrients. Interday variability in microbial plankton responses to B vitamins was limited compared to interseason variability, suggesting that B-vitamin availability might be partially controlled by factors operating at seasonal scale. Chlorophyll a (Chl a ) concentration and prokaryote biomass (PB) significantly increased after B-vitamin amendments in 13 % and 21 %, respectively, of the 216 cases (36 experiments × 6 treatments). Most of these positive responses were produced by treatments containing either B12 solely or B12 combined with B1 in oceanic waters, which was consistent with the significantly lower average vitamin-B12 ambient concentrations compared to that in the coastal station. Negative responses, implying a decrease in Chl a or PB, represented 21 % for phytoplankton and 26 % for prokaryotes. Growth stimulation by B1 addition was more frequent on prokaryotes than in phytoplankton, suggesting that B1 auxotrophy in the sampling area could be more widespread in prokaryotes than in phytoplankton. Negative responses to B vitamins were generalized in coastal surface waters in summer and were associated with a high contribution of Flavobacteriales to the prokaryote community. This observation suggests that the external supply of B12 and/or B1 may promote negative interactions between microbial components when B-vitamin auxotrophs are abundant. The microbial response patterns to B12 and/or B1 amendments were significantly correlated with changes in the prokaryotic community composition, highlighting the pivotal role of prokaryotes in B-vitamin cycling in marine ecosystems. Article in Journal/Newspaper Northeast Atlantic Directory of Open Access Journals: DOAJ Articles Biogeosciences 17 10 2807 2823 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
spellingShingle |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 V. Joglar A. Prieto E. Barber-Lluch M. Hernández-Ruiz E. Fernández E. Teira Spatial and temporal variability in the response of phytoplankton and prokaryotes to B-vitamin amendments in an upwelling system |
topic_facet |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
description |
We experimentally evaluated the temporal (interday and interseason) and spatial variability in microbial plankton responses to vitamin B12 and/or B1 supply (solely or in combination with inorganic nutrients) in coastal and oceanic waters of the northeast Atlantic Ocean. Phytoplankton and, to a lesser extent, prokaryotes were strongly limited by inorganic nutrients. Interday variability in microbial plankton responses to B vitamins was limited compared to interseason variability, suggesting that B-vitamin availability might be partially controlled by factors operating at seasonal scale. Chlorophyll a (Chl a ) concentration and prokaryote biomass (PB) significantly increased after B-vitamin amendments in 13 % and 21 %, respectively, of the 216 cases (36 experiments × 6 treatments). Most of these positive responses were produced by treatments containing either B12 solely or B12 combined with B1 in oceanic waters, which was consistent with the significantly lower average vitamin-B12 ambient concentrations compared to that in the coastal station. Negative responses, implying a decrease in Chl a or PB, represented 21 % for phytoplankton and 26 % for prokaryotes. Growth stimulation by B1 addition was more frequent on prokaryotes than in phytoplankton, suggesting that B1 auxotrophy in the sampling area could be more widespread in prokaryotes than in phytoplankton. Negative responses to B vitamins were generalized in coastal surface waters in summer and were associated with a high contribution of Flavobacteriales to the prokaryote community. This observation suggests that the external supply of B12 and/or B1 may promote negative interactions between microbial components when B-vitamin auxotrophs are abundant. The microbial response patterns to B12 and/or B1 amendments were significantly correlated with changes in the prokaryotic community composition, highlighting the pivotal role of prokaryotes in B-vitamin cycling in marine ecosystems. |
format |
Article in Journal/Newspaper |
author |
V. Joglar A. Prieto E. Barber-Lluch M. Hernández-Ruiz E. Fernández E. Teira |
author_facet |
V. Joglar A. Prieto E. Barber-Lluch M. Hernández-Ruiz E. Fernández E. Teira |
author_sort |
V. Joglar |
title |
Spatial and temporal variability in the response of phytoplankton and prokaryotes to B-vitamin amendments in an upwelling system |
title_short |
Spatial and temporal variability in the response of phytoplankton and prokaryotes to B-vitamin amendments in an upwelling system |
title_full |
Spatial and temporal variability in the response of phytoplankton and prokaryotes to B-vitamin amendments in an upwelling system |
title_fullStr |
Spatial and temporal variability in the response of phytoplankton and prokaryotes to B-vitamin amendments in an upwelling system |
title_full_unstemmed |
Spatial and temporal variability in the response of phytoplankton and prokaryotes to B-vitamin amendments in an upwelling system |
title_sort |
spatial and temporal variability in the response of phytoplankton and prokaryotes to b-vitamin amendments in an upwelling system |
publisher |
Copernicus Publications |
publishDate |
2020 |
url |
https://doi.org/10.5194/bg-17-2807-2020 https://doaj.org/article/5d54b03b82834f9dad805b28951ea04c |
genre |
Northeast Atlantic |
genre_facet |
Northeast Atlantic |
op_source |
Biogeosciences, Vol 17, Pp 2807-2823 (2020) |
op_relation |
https://www.biogeosciences.net/17/2807/2020/bg-17-2807-2020.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 doi:10.5194/bg-17-2807-2020 1726-4170 1726-4189 https://doaj.org/article/5d54b03b82834f9dad805b28951ea04c |
op_doi |
https://doi.org/10.5194/bg-17-2807-2020 |
container_title |
Biogeosciences |
container_volume |
17 |
container_issue |
10 |
container_start_page |
2807 |
op_container_end_page |
2823 |
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1766143294086053888 |