SiFBA5, a cold-responsive factor from Saussurea involucrata promotes cold resilience and biomass increase in transgenic tomato plants under cold stress
Abstract Background Saussurea involucrata survives in extreme arctic conditions and is very cold-resistant. This species grows in rocky, mountainous areas with elevations of 2400–4100 m, which are snow-covered year-round and are subject to freezing temperatures. S. involucrata’s ability to survive i...
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ftdoajarticles:oai:doaj.org/article:5d364f284abe44aaab111fc256be6b6c 2023-05-15T15:13:06+02:00 SiFBA5, a cold-responsive factor from Saussurea involucrata promotes cold resilience and biomass increase in transgenic tomato plants under cold stress Jianqiang Mu Yajuan Fu Bucang Liu Yao Zhang Aiying Wang Yuxia Li Jianbo Zhu 2021-02-01T00:00:00Z https://doi.org/10.1186/s12870-021-02851-8 https://doaj.org/article/5d364f284abe44aaab111fc256be6b6c EN eng BMC https://doi.org/10.1186/s12870-021-02851-8 https://doaj.org/toc/1471-2229 doi:10.1186/s12870-021-02851-8 1471-2229 https://doaj.org/article/5d364f284abe44aaab111fc256be6b6c BMC Plant Biology, Vol 21, Iss 1, Pp 1-10 (2021) SiFBA5 Calvin cycle Photosynthesis Cold stress Chlorophyll Botany QK1-989 article 2021 ftdoajarticles https://doi.org/10.1186/s12870-021-02851-8 2022-12-31T05:38:57Z Abstract Background Saussurea involucrata survives in extreme arctic conditions and is very cold-resistant. This species grows in rocky, mountainous areas with elevations of 2400–4100 m, which are snow-covered year-round and are subject to freezing temperatures. S. involucrata’s ability to survive in an extreme low-temperature environment suggests that it has particularly high photosynthetic efficiency, providing a magnificent model, and rich gene pool, for the analysis of plant cold stress response. Fructose-1, 6-bisphosphate aldolase (FBA) is a key enzyme in the photosynthesis process and also mediates the conversion of fructose 1, 6-bisphosphate (FBP) into dihydroxyacetone phosphate (DHAP) and glycerol triphosphate (GAP) during glycolysis and gluconeogenesis. The molecular mechanisms underlying S. involucrata’s cold tolerance are still unclear; therefore, our work aims to investigate the role of FBA in plant cold-stress response. Results In this study, we identified a cold-responsive gene, SiFBA5, based on a preliminary low-temperature, genome-wide transcriptional profiling of S. involucrata. Expression analysis indicated that cold temperatures rapidly induced transcriptional expression of SiFBA5, suggesting that SiFBA5 participates in the initial stress response. Subcellular localization analysis revealed that SiFBA5 is localized to the chloroplast. Transgenic tomato plants that overexpressed SiFBA5 were generated using a CaMV 35S promoter. Phenotypic observation suggested that the transgenic plants displayed increased cold tolerance and photosynthetic efficiency in comparison with wild-type plants. Conclusion Cold stress has a detrimental impact on crop yield. Our results demonstrated that SiFBA5 positively regulates plant response to cold stress, which is of great significance for increasing crop yield under cold stress conditions. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Calvin ENVELOPE(165.100,165.100,-71.283,-71.283) BMC Plant Biology 21 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
SiFBA5 Calvin cycle Photosynthesis Cold stress Chlorophyll Botany QK1-989 |
spellingShingle |
SiFBA5 Calvin cycle Photosynthesis Cold stress Chlorophyll Botany QK1-989 Jianqiang Mu Yajuan Fu Bucang Liu Yao Zhang Aiying Wang Yuxia Li Jianbo Zhu SiFBA5, a cold-responsive factor from Saussurea involucrata promotes cold resilience and biomass increase in transgenic tomato plants under cold stress |
topic_facet |
SiFBA5 Calvin cycle Photosynthesis Cold stress Chlorophyll Botany QK1-989 |
description |
Abstract Background Saussurea involucrata survives in extreme arctic conditions and is very cold-resistant. This species grows in rocky, mountainous areas with elevations of 2400–4100 m, which are snow-covered year-round and are subject to freezing temperatures. S. involucrata’s ability to survive in an extreme low-temperature environment suggests that it has particularly high photosynthetic efficiency, providing a magnificent model, and rich gene pool, for the analysis of plant cold stress response. Fructose-1, 6-bisphosphate aldolase (FBA) is a key enzyme in the photosynthesis process and also mediates the conversion of fructose 1, 6-bisphosphate (FBP) into dihydroxyacetone phosphate (DHAP) and glycerol triphosphate (GAP) during glycolysis and gluconeogenesis. The molecular mechanisms underlying S. involucrata’s cold tolerance are still unclear; therefore, our work aims to investigate the role of FBA in plant cold-stress response. Results In this study, we identified a cold-responsive gene, SiFBA5, based on a preliminary low-temperature, genome-wide transcriptional profiling of S. involucrata. Expression analysis indicated that cold temperatures rapidly induced transcriptional expression of SiFBA5, suggesting that SiFBA5 participates in the initial stress response. Subcellular localization analysis revealed that SiFBA5 is localized to the chloroplast. Transgenic tomato plants that overexpressed SiFBA5 were generated using a CaMV 35S promoter. Phenotypic observation suggested that the transgenic plants displayed increased cold tolerance and photosynthetic efficiency in comparison with wild-type plants. Conclusion Cold stress has a detrimental impact on crop yield. Our results demonstrated that SiFBA5 positively regulates plant response to cold stress, which is of great significance for increasing crop yield under cold stress conditions. |
format |
Article in Journal/Newspaper |
author |
Jianqiang Mu Yajuan Fu Bucang Liu Yao Zhang Aiying Wang Yuxia Li Jianbo Zhu |
author_facet |
Jianqiang Mu Yajuan Fu Bucang Liu Yao Zhang Aiying Wang Yuxia Li Jianbo Zhu |
author_sort |
Jianqiang Mu |
title |
SiFBA5, a cold-responsive factor from Saussurea involucrata promotes cold resilience and biomass increase in transgenic tomato plants under cold stress |
title_short |
SiFBA5, a cold-responsive factor from Saussurea involucrata promotes cold resilience and biomass increase in transgenic tomato plants under cold stress |
title_full |
SiFBA5, a cold-responsive factor from Saussurea involucrata promotes cold resilience and biomass increase in transgenic tomato plants under cold stress |
title_fullStr |
SiFBA5, a cold-responsive factor from Saussurea involucrata promotes cold resilience and biomass increase in transgenic tomato plants under cold stress |
title_full_unstemmed |
SiFBA5, a cold-responsive factor from Saussurea involucrata promotes cold resilience and biomass increase in transgenic tomato plants under cold stress |
title_sort |
sifba5, a cold-responsive factor from saussurea involucrata promotes cold resilience and biomass increase in transgenic tomato plants under cold stress |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s12870-021-02851-8 https://doaj.org/article/5d364f284abe44aaab111fc256be6b6c |
long_lat |
ENVELOPE(165.100,165.100,-71.283,-71.283) |
geographic |
Arctic Calvin |
geographic_facet |
Arctic Calvin |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
BMC Plant Biology, Vol 21, Iss 1, Pp 1-10 (2021) |
op_relation |
https://doi.org/10.1186/s12870-021-02851-8 https://doaj.org/toc/1471-2229 doi:10.1186/s12870-021-02851-8 1471-2229 https://doaj.org/article/5d364f284abe44aaab111fc256be6b6c |
op_doi |
https://doi.org/10.1186/s12870-021-02851-8 |
container_title |
BMC Plant Biology |
container_volume |
21 |
container_issue |
1 |
_version_ |
1766343705128599552 |