Vector Competence of American Mosquitoes for Three Strains of Zika Virus.
In 2015, Zika virus (ZIKV; Flaviviridae; Flavivirus) emerged in the Americas, causing millions of infections in dozens of countries. The rapid spread of the virus and the association with disease outcomes such as Guillain-Barré syndrome and microcephaly make understanding transmission dynamics essen...
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ftdoajarticles:oai:doaj.org/article:5d04ea0427f0403799b3cad00751f1f3 2023-05-15T15:13:34+02:00 Vector Competence of American Mosquitoes for Three Strains of Zika Virus. James Weger-Lucarelli Claudia Rückert Nunya Chotiwan Chilinh Nguyen Selene M Garcia Luna Joseph R Fauver Brian D Foy Rushika Perera William C Black Rebekah C Kading Gregory D Ebel 2016-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005101 https://doaj.org/article/5d04ea0427f0403799b3cad00751f1f3 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5081193?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005101 https://doaj.org/article/5d04ea0427f0403799b3cad00751f1f3 PLoS Neglected Tropical Diseases, Vol 10, Iss 10, p e0005101 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005101 2022-12-31T12:11:29Z In 2015, Zika virus (ZIKV; Flaviviridae; Flavivirus) emerged in the Americas, causing millions of infections in dozens of countries. The rapid spread of the virus and the association with disease outcomes such as Guillain-Barré syndrome and microcephaly make understanding transmission dynamics essential. Currently, there are no reports of vector competence (VC) of American mosquitoes for ZIKV isolates from the Americas. Further, it is not clear whether ZIKV strains from other genetic lineages can be transmitted by American Aedes aegypti populations, and whether the scope of the current epidemic is in part facilitated by viral factors such as enhanced replicative fitness or increased vector competence. Therefore, we characterized replication of three ZIKV strains, one from each of the three phylogenetic clades in several cell lines and assessed their abilities to be transmitted by Ae. aegypti mosquitoes. Additionally, laboratory colonies of different Culex spp. were infected with an American outbreak strain of ZIKV to assess VC. Replication rates were variable and depended on virus strain, cell line and MOI. African strains used in this study outcompeted the American strain in vitro in both mammalian and mosquito cell culture. West and East African strains of ZIKV tested here were more efficiently transmitted by Ae. aegypti from Mexico than was the currently circulating American strain of the Asian lineage. Long-established laboratory colonies of Culex mosquitoes were not efficient ZIKV vectors. These data demonstrate the capacity for additional ZIKV strains to infect and replicate in American Aedes mosquitoes and suggest that neither enhanced virus replicative fitness nor virus adaptation to local vector mosquitoes seems likely to explain the extent and intensity of ZIKV transmission in the Americas. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Barré ENVELOPE(-68.550,-68.550,-67.500,-67.500) PLOS Neglected Tropical Diseases 10 10 e0005101 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 James Weger-Lucarelli Claudia Rückert Nunya Chotiwan Chilinh Nguyen Selene M Garcia Luna Joseph R Fauver Brian D Foy Rushika Perera William C Black Rebekah C Kading Gregory D Ebel Vector Competence of American Mosquitoes for Three Strains of Zika Virus. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
In 2015, Zika virus (ZIKV; Flaviviridae; Flavivirus) emerged in the Americas, causing millions of infections in dozens of countries. The rapid spread of the virus and the association with disease outcomes such as Guillain-Barré syndrome and microcephaly make understanding transmission dynamics essential. Currently, there are no reports of vector competence (VC) of American mosquitoes for ZIKV isolates from the Americas. Further, it is not clear whether ZIKV strains from other genetic lineages can be transmitted by American Aedes aegypti populations, and whether the scope of the current epidemic is in part facilitated by viral factors such as enhanced replicative fitness or increased vector competence. Therefore, we characterized replication of three ZIKV strains, one from each of the three phylogenetic clades in several cell lines and assessed their abilities to be transmitted by Ae. aegypti mosquitoes. Additionally, laboratory colonies of different Culex spp. were infected with an American outbreak strain of ZIKV to assess VC. Replication rates were variable and depended on virus strain, cell line and MOI. African strains used in this study outcompeted the American strain in vitro in both mammalian and mosquito cell culture. West and East African strains of ZIKV tested here were more efficiently transmitted by Ae. aegypti from Mexico than was the currently circulating American strain of the Asian lineage. Long-established laboratory colonies of Culex mosquitoes were not efficient ZIKV vectors. These data demonstrate the capacity for additional ZIKV strains to infect and replicate in American Aedes mosquitoes and suggest that neither enhanced virus replicative fitness nor virus adaptation to local vector mosquitoes seems likely to explain the extent and intensity of ZIKV transmission in the Americas. |
format |
Article in Journal/Newspaper |
author |
James Weger-Lucarelli Claudia Rückert Nunya Chotiwan Chilinh Nguyen Selene M Garcia Luna Joseph R Fauver Brian D Foy Rushika Perera William C Black Rebekah C Kading Gregory D Ebel |
author_facet |
James Weger-Lucarelli Claudia Rückert Nunya Chotiwan Chilinh Nguyen Selene M Garcia Luna Joseph R Fauver Brian D Foy Rushika Perera William C Black Rebekah C Kading Gregory D Ebel |
author_sort |
James Weger-Lucarelli |
title |
Vector Competence of American Mosquitoes for Three Strains of Zika Virus. |
title_short |
Vector Competence of American Mosquitoes for Three Strains of Zika Virus. |
title_full |
Vector Competence of American Mosquitoes for Three Strains of Zika Virus. |
title_fullStr |
Vector Competence of American Mosquitoes for Three Strains of Zika Virus. |
title_full_unstemmed |
Vector Competence of American Mosquitoes for Three Strains of Zika Virus. |
title_sort |
vector competence of american mosquitoes for three strains of zika virus. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0005101 https://doaj.org/article/5d04ea0427f0403799b3cad00751f1f3 |
long_lat |
ENVELOPE(-68.550,-68.550,-67.500,-67.500) |
geographic |
Arctic Barré |
geographic_facet |
Arctic Barré |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 10, p e0005101 (2016) |
op_relation |
http://europepmc.org/articles/PMC5081193?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005101 https://doaj.org/article/5d04ea0427f0403799b3cad00751f1f3 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005101 |
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PLOS Neglected Tropical Diseases |
container_volume |
10 |
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10 |
container_start_page |
e0005101 |
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1766344106957602816 |